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  4. Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 12 (Ptpn12)

Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 12 (Ptpn12)

  • 中文名称：
    小鼠Ptpn12重组蛋白
  • 货号：
    CSB-YP019026MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ptpn12重组蛋白
  • 货号：
    CSB-EP019026MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Ptpn12重组蛋白
  • 货号：
    CSB-EP019026MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ptpn12重组蛋白
  • 货号：
    CSB-BP019026MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ptpn12重组蛋白
  • 货号：
    CSB-MP019026MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Ptpn12
  • Uniprot No.：
  • 别名：
    Ptpn12; Tyrosine-protein phosphatase non-receptor type 12; EC 3.1.3.48; MPTP-PEST; Protein-tyrosine phosphatase P19; P19-PTP
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-775
  • 氨基酸序列
    MEQVEILRRF IQRVQAMKSP DHNGEDNFAR DFMRLRRLST KYRTEKIYPT ATGEKEENVK KNRYKDILPF DHSRVKLTLK TPSQDSDYIN ANFIKGVYGP KAYVATQGPL ANTVIDFWRM IWEYNVVIIV MACREFEMGR KKCERYWPLY GEDPITFAPF KISCENEQAR TDYFIRTLLL EFQNESRRLY QFHYVNWPDH DVPSSFDSIL DMISLMRKYQ EHEDVPICIH CSAGCGRTGA ICAIDYTWNL LKAGKIPEEF NVFNLIQEMR TQRHSAVQTK EQYELVHRAI AQLFEKQLQL YEIHGAQKIA DGNEITTGTM VSSIDSEKQD SPPPKPPRTR SCLVEGDAKE EILQPPEPHP VPPILTPSPP SAFPTVTTVW QDSDRYHPKP VLHMASPEQH PADLNRSYDK SADPMGKSES AIEHIDKKLE RNLSFEIKKV PLQEGPKSFD GNTLLNRGHA IKIKSASSSV VDRTSKPQEL SAGALKVDDV SQNSCADCSA AHSHRAAESS EESQSNSHTP PRPDCLPLDK KGHVTWSLHG PENATPVPDS PDGKSPDNHS QTLKTVSSTP NSTAEEEAHD LTEHHNSSPL LKAPLSFTNP LHSDDSDSDG GSSDGAVTRN KTSISTASAT VSPASSAESA CTRRVLPMSI ARQEVAGTPH SGAEKDADVS EESPPPLPER TPESFVLADM PVRPEWHELP NQEWSEQRES EGLTTSGNEK HDAGGIHTEA SADSPPAFSD KKDQITKSPA EVTDIGFGNR CGKPKGPREP PSEWT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades. Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2. Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248'.
  • 基因功能参考文献：
    1. PTPN12-deficient, ErbB2-dependent breast cancer cells were more resistant to anoikis and had augmented migratory and invasive properties. PMID: 26391955
    2. Integrin beta 8 and PTP-PEST form protein complexes at the leading edge of migrating cells and balance patterns of Rac1 and Cdc42 signaling by controlling the subcellular localization and phosphorylation status of Rho GDP dissociation inhibitor 1. PMID: 25666508
    3. Pyk2 deregulation played a pivotal role in the migration defect caused by PTPN12 deficiency. PMID: 24366546
    4. Regulation of the Src kinase-associated phosphoprotein 55 homologue by the protein tyrosine phosphatase PTP-PEST in the control of cell motility. PMID: 23897807
    5. macrophage fusion is critically dependent on PTP-PEST. PMID: 23589331
    6. Overexpression of PTP-PEST substantially inhibits lipopolysaccharide (LPS)-induced tyrosine phosphorylation of IkappaB kinase (IKKbeta) at Tyr188/199 in RAW264.7 cells. PMID: 23296707
    7. PTP-PEST is a key regulator of integrin-mediated functions in endothelial cells. PMID: 23105101
    8. the paxillin-PTP-PEST interaction contributes to integrin signaling PMID: 16317044
    9. PTP-PEST plays an essential function in early embryogenesis. PMID: 17070019
    10. PTP-PEST actively contributes to the cellular apoptotic response and reveal the importance of caspases as regulators of PTPs in apoptosis. PMID: 17130234
    11. PTP-PEST tempers T cell activation by dephosphorylating T cell receptor (TCR)-proximal signaling molecules, such as Lck, and that down-regulation of PTP-PEST may be a reason for the increased response to TCR triggering of previously activated T cells. PMID: 18457880
    12. Results show a regulatory role for PTP-PEST on dephosphorylation of c-Src at Y527 and phosphorylation at Y418 in the catalytic site. PMID: 19350555
    13. Data show that ERK phosphorylates FAK S910 and recruits PIN1 and PTP-PEST, which colocalize with FAK at the lamellipodia of migrating cells. PMID: 19595712

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  • 亚细胞定位：
    Cytoplasm. Cell junction, focal adhesion. Cell projection, podosome.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class 4 subfamily
  • 数据库链接：

    KEGG: mmu:19248

    STRING: 10090.ENSMUSP00000030556

    UniGene: Mm.319117