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  4. Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 6 (Ptpn6)

Recombinant Mouse Tyrosine-protein phosphatase non-receptor type 6 (Ptpn6)

  • 中文名称：
    小鼠Ptpn6重组蛋白
  • 货号：
    CSB-YP019043MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Ptpn6重组蛋白
  • 货号：
    CSB-EP019043MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Ptpn6重组蛋白
  • 货号：
    CSB-BP019043MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Ptpn6重组蛋白
  • 货号：
    CSB-MP019043MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ptpn6; Hcp; Hcph; Ptp1C; Tyrosine-protein phosphatase non-receptor type 6; EC 3.1.3.48; 70Z-SHP; Hematopoietic cell protein-tyrosine phosphatase; PTPTY-42; Protein-tyrosine phosphatase 1C; PTP-1C; SH-PTP1; SHP-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-595
  • 氨基酸序列
    MVRWFHRDLS GPDAETLLKG RGVPGSFLAR PSRKNQGDFS LSVRVDDQVT HIRIQNSGDF YDLYGGEKFA TLTELVEYYT QQQGILQDRD GTIIHLKYPL NCSDPTSERW YHGHISGGQA ESLLQAKGEP WTFLVRESLS QPGDFVLSVL NDQPKAGPGS PLRVTHIKVM CEGGRYTVGG SETFDSLTDL VEHFKKTGIE EASGAFVYLR QPYYATRVNA ADIENRVLEL NKKQESEDTA KAGFWEEFES LQKQEVKNLH QRLEGQRPEN KSKNRYKNIL PFDHSRVILQ GRDSNIPGSD YINANYVKNQ LLGPDENSKT YIASQGCLDA TVNDFWQMAW QENTRVIVMT TREVEKGRNK CVPYWPEVGT QRVYGLYSVT NSREHDTAEY KLRTLQISPL DNGDLVREIW HYQYLSWPDH GVPSEPGGVL SFLDQINQRQ ESLPHAGPII VHCSAGIGRT GTIIVIDMLM ESISTKGLDC DIDIQKTIQM VRAQRSGMVQ TEAQYKFIYV AIAQFIETTK KKLEIIQSQK GQESEYGNIT YPPAVRSAHA KASRTSSKHK EEVYENVHSK SKKEEKVKKQ RSADKEKNKG SLKRK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis.
  • 基因功能参考文献：
    1. Deletion of AT2 receptor reduced SHP-1 activity and restored VEGF actions, leading to an increased blood flow reperfusion after ischemia in diabetes mellitus. PMID: 29074590
    2. These findings show a novel role for Shp-1 in the regulation of IEC growth and secretory lineage allocation, possibly via modulation of PI3K/Akt-dependent signaling pathways. PMID: 28465325
    3. These findings suggest that protein tyrosine phosphatase SHP-1 may act as a positive regulator of osteoblast differentiation through direct association with and dephosphorylation of GSK3beta. PMID: 27614023
    4. data establish SHP-1 as a critical player in setting the threshold downstream of TCR signaling and identify a novel function of SHP-1 as a regulator of T cell susceptibility to Treg-mediated suppression in vitro and in vivo PMID: 28550200
    5. Results are consistent with predicted/observed reduction in the Lyn-SHIP-1-PTEN-SHP-1 axis function in B cells from systemic lupus PMID: 27114609
    6. Our data show that SHP1 is required for the survival of mature thymocytes and the generation of the functional T-cell repertoire, as its absence leads to a reduction in the numbers of CD4(+) and CD8(+) naive T cells in the peripheral lymphoid compartments. PMID: 27354309
    7. Our study suggests that metformin exerts its insulin sensitizing effects via inhibition of SHP-1 activity and expression. PMID: 28389241
    8. we found that THEMIS directly regulated the catalytic activity of the tyrosine phosphatase SHP-1. PMID: 28250424
    9. Studies indicate that SHP1 and SYK crosstalk as a critical regulator of MyD88 post-translational modifications and IL-1-driven inflammation. PMID: 28410990
    10. Our findings uncover an important role for PP6 as an indispensable guardian of genomic integrity of the lengthy prophase I oocyte arrest, maintenance of primordial follicle pool, and thus female fertility. PMID: 27930667
    11. The goal of this study was to analyze differentially expressed genes in the bone marrow of mice with NDLD to gain insight into the role of Ptpn6 in myelopoietic bone marrow pathology, and the mechanisms by which Ptpn6 insufficiency in the hematopoietic cells can lead to the development of skin lesions. PMID: 27020408
    12. In addition to their role in NK cell activation by hematopoietic cells, the SLAM-SAP-SHP1 pathways influence responsiveness toward nonhematopoietic targets by a process akin to NK cell 'education'. PMID: 26878112
    13. The prostate of mev/mev mice exhibits signs of aberrant differentiation and the resulting phenotype may be related to the loss of function of SHP-1. Prostatic anomalies in these mice affect, together with defects in sperm maduration, for their sterility. PMID: 24819344
    14. These studies reveal critical pathways controlled by SHP-1 in oligodendrocytes that relate to susceptibility of SHP-1-deficient mice to both developmental defects in myelination and to inflammatory demyelinating diseases PMID: 25919645
    15. SHP-1 regulates Cbl-b-mediated T cell responses by controlling its tyrosine phosphorylation and ubiquitination PMID: 26416283
    16. The inhibitory activity of SHP-1 is needed for setting the threshold of natural killer cell reactivity. PMID: 25355530
    17. Shp1 signalling is required for the establishment of a life-long protective humoral immunity. PMID: 24978161
    18. Shp1 binds and controls PIPKIgamma activity and, thereby, modulates phosphatidylinositol (4,5)-bisphosphate levels and adhesion. PMID: 26101325
    19. CCN1-integrin binding increased the expression of and association between SHP-1 and VEGF-R2, which leads to rapid dephosphorylation of VEGF-R2 tyrosine, thus preventing endothelial cell hyperproliferation. PMID: 26002917
    20. pY motifs from known SHP-1 and SHP-2-binding proteins show that many of the pY motifs contain a hydrophobic or positively charged residue(s) at the pY+4 and pY+5 positions PMID: 16702225
    21. SHP-1 appears to alter multiple aspects of macrophage biology that control virus replication, chemokine responsiveness, and inflammatory activity, that directly relate to virus-induced demyelinating disease. PMID: 19951174
    22. our data reveal SHP-1 as a critical modifier of Treg function PMID: 20952680
    23. these findings demonstrate that infiltrating macrophages in SHP-1-deficient mice play a crucial role in promoting viral replication and contribute to increased proinflammatory gene expression leading to demyelination. PMID: 18987138
    24. there is a critical role for the tyrosine phosphatase activity of SHP-1 for induction of IL-12p40 production in macrophages in response to TLR ligands PMID: 20145200
    25. In mouse liver, transcriptional activation of SHP1 gene by Prep1 attenuates insulin signal transduction and reduces glucose storage. PMID: 20864515
    26. data reveal that PD-L1 is a critical modulator of Tregs' ability to suppress iALI, and this appears to involve SHP-1 activation. PMID: 25057927
    27. Data indicate a pathological role for protein tyrosine phosphatase non-receptor type 6 SHP-1 in promoting inflammatory macrophage differentiation and myofiber damage in virus-infected skeletal muscle. PMID: 25681345
    28. SHP-1 enzyme and Th2/Th1 paradigm may play a critical role in the maintenance of nasal immune homeostasis and in the regulation of allergic rhinitis. PMID: 25090641
    29. SHP-1 contributes to nephrin deactivation in podocytes exposed to high glucose levels. PMID: 25404734
    30. These results indicate that JBP regulates the expressions of SHP-1, Wnt3a, and AP-1 proteins in chemically damaged mice. PMID: 24841652
    31. an SHP-1-centered feedback system wherein SHP-1 modulates CD40-induced p38MAPK activation threshold and reciprocal ERK-1/2 activation, establishing itself as a critical regulator of CD40 signaling reciprocity PMID: 25187664
    32. STAT3 was a substrate for SHP-1 by co-immunoprecipitation. PMID: 24466030
    33. We demonstrated that the association and oxidation of c-Src and SHP-1 by ROS are key steps in enhancing OC survival, which are responsible for increased bone loss when ovarian function ceases. PMID: 24824657
    34. in DKO pre-B cells, the kinase Zap70 is associated with the pre-BCR, suggesting that Zap70 is important to promote B cell maturation in the absence of Syk and SHP-1. PMID: 24899508
    35. a novel role for hepatocyte Shp1 in the regulation of PPARgamma and hepatic lipid metabolism. PMID: 24327268
    36. The endothelial tyrosine phosphatase SHP-1 plays an important role for vascular hemostasis in vivo, which is crucial in TNF alpha -induced endothelial inflammation. PMID: 23766558
    37. our results show for the first time that SHP-1 acts as a positive regulator of LPS-induced IL-10 production in splenic macrophages PMID: 23904162
    38. Activation of Shp1-deficient CD4+ T cells results in skewing to the Th2 lineage and increased IL-4 production. PMID: 23797092
    39. FGFRL1 does not function as a decoy receptor in beta-cells, but rather it enhances ERK1/2 signaling through association of SHP-1 with the receptor's intracellular SH2-binding motif. PMID: 23640895
    40. Shp1 and Shp2 are major regulators of megakaryocyte development, platelet production, and function. PMID: 23509158
    41. high levels of SHP-1 expression in glomeruli cause insulin resistance and podocyte loss PMID: 23531619
    42. SHP-1 plays a critical role as a negative regulator in allergic inflammation, in allergen induced anaphylaxis, and seems to be required for normal basophil development. PMID: 23390550
    43. Advanced glycation end product Nepsilon-carboxymethyllysine induces endothelial cell injuryinvolving SHP-1-regulated VEGFR-2 dephosphorylation. PMID: 22553146
    44. dendritic cells functionally educate iNKT cells by tuning SHP-1 expression to limit reactivity. PMID: 23427253
    45. Data indicate the amounts of Ptpn6 (Shp1) and MyD88 protein were reduced in dendritic cells of Ptpn6fl/flMyd88fl/flItgax-cre mice. PMID: 23521885
    46. Selective target recognition of the SHP-1 knockdown natural killer (NK) cells revealed also possible involvement of the SHP-1 phosphatase in regulating other NK functions in mature NK cells. PMID: 22952938
    47. findings show that most proliferating Germinal center (GC) B cells did not demonstrate active B cell receptor(BCR)signaling; signaling was limited by increased phosphatase activity; both SHP-1 and SHIP-1 were hyperphosphorylated in GC cells and remained colocalized with BCRs after ligation; SHP-1 was required for GC maintenance PMID: 22555432
    48. SHP-1 is recruited early to phagosomes and that it is required for the acquisition of macrophage lysosomal features and acidification. PMID: 22826316
    49. These studies suggest that abrogating SHP-1 in effector T cells may improve the efficacy of tumor elimination by T cell therapy without affecting the ability of the effector cells to persist and provide a long-term response. PMID: 22798667
    50. Hyperglycemia and diabetes can cause glomerular podocyte apoptosis and endothelial dysfunction partly due to increased PKCdelta/p38 MAPK activation and the expression of SHP-1 to cause VEGF resistance, independent of NF-kappaB activation. PMID: 22499584

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  • 相关疾病：
    Defects in Ptpn6 are the cause of the motheaten (me) or viable motheaten (mev) phenotypes. Mice homozygous for the recessive allelic mutations develop severe defects in hematopoiesis.
  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    Protein-tyrosine phosphatase family, Non-receptor class 2 subfamily
  • 组织特异性：
    Expressed predominantly in hematopoietic cells.
  • 数据库链接：

    KEGG: mmu:15170

    STRING: 10090.ENSMUSP00000004377

    UniGene: Mm.271799