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  4. Recombinant Mouse Tyrosyl-DNA phosphodiesterase 2 (Tdp2)

Recombinant Mouse Tyrosyl-DNA phosphodiesterase 2 (Tdp2)

  • 中文名称：
    小鼠Tdp2重组蛋白
  • 货号：
    CSB-YP872861MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Tdp2重组蛋白
  • 货号：
    CSB-EP872861MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Tdp2重组蛋白
  • 货号：
    CSB-EP872861MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Tdp2重组蛋白
  • 货号：
    CSB-BP872861MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Tdp2重组蛋白
  • 货号：
    CSB-MP872861MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Tdp2
  • Uniprot No.：
  • 别名：
    Tdp2; Ttrap; Tyrosyl-DNA phosphodiesterase 2; Tyr-DNA phosphodiesterase 2; EC 3.1.4.-; 5'-tyrosyl-DNA phosphodiesterase; 5'-Tyr-DNA phosphodiesterase; TRAF and TNF receptor-associated protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-370
  • 氨基酸序列
    MASGSSSDAA EPAGPAGRAA SAPEAAQAEE DRVKRRRLQC LGFALVGGCD PTMVPSVLRE NDWQTQKALS AYFELPENDQ GWPRQPPTSF KSEAYVDLTN EDANDTTILE ASPSGTPLED SSTISFITWN IDGLDGCNLP ERARGVCSCL ALYSPDVVFL QEVIPPYCAY LKKRAASYTI ITGNEEGYFT AILLKKGRVK FKSQEIIPFP NTKMMRNLLC VNVSLGGNEF CLMTSHLEST REHSAERIRQ LKTVLGKMQE APDSTTVIFA GDTNLRDQEV IKCGGLPDNV FDAWEFLGKP KHCQYTWDTK ANNNLRIPAA YKHRFDRIFF RAEEGHLIPQ SLDLVGLEKL DCGRFPSDHW GLLCTLNVVL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress.
  • 基因功能参考文献：
    1. EMCV yields are significantly reduced during infection of mouse embryonic fibroblasts genetically ablated for TDP2. PMID: 29353210
    2. The results collectively provide a structural mechanism for Tdp2 engagement of heterogeneous DNA damage that causes Top2 poisoning. PMID: 27060144
    3. data show that TDP2 activity potentiates enterovirus infections and, in the case of coxsackievirus B3, is a required host function following virus infection of mouse cells PMID: 26715620
    4. Evidence implicates Tdp2 in the repair of Top1-mediated DNA damage in cultured cells and in vivo, in the absence of Tdp1. PMID: 22740648
    5. the protein associates with TGF-beta receptors and components of the TRAF6-TAK1 signaling module PMID: 21980489

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  • 亚细胞定位：
    Nucleus. Nucleus, PML body. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族：
    CCR4/nocturin family
  • 组织特异性：
    Widely expressed. Expressed in whole brain, cerebellum, quiescent cortical astrocytes and cerebellar granule neurons.
  • 数据库链接：

    KEGG: mmu:56196

    STRING: 10090.ENSMUSP00000035660

    UniGene: Mm.427162