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  4. Recombinant Mouse Ubiquitin-like modifier-activating enzyme ATG7 (Atg7)

Recombinant Mouse Ubiquitin-like modifier-activating enzyme ATG7 (Atg7)

  • 货号：
    CSB-YP884031MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP884031MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP884031MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP884031MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP884031MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Atg7; Apg7lUbiquitin-like modifier-activating enzyme ATG7; ATG12-activating enzyme E1 ATG7; Autophagy-related protein 7; APG7-like; mAGP7; Ubiquitin-activating enzyme E1-like protein
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-698
  • 氨基酸序列
    MGDPGLAKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG DSAGLPTRLT LEFSAFDMSA STPAHCCPAM GTLHNTNTLE AFKTADKKLL LEQSANEIWE AIKSGAALEN PMLLNKFLLL TFADLKKYHF YYWFCCPALC LPESIPLIRG PVSLDQRLSP KQIQALEHAY DDLCRAEGVT ALPYFLFKYD DDTVLVSLLK HYSDFFQGQR TKITVGVYDP CNLAQYPGWP LRNFLVLAAH RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV SVKCLLLGAG TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE DCLGGGKPKA LAAAERLQKI FPGVNARGFN MSIPMPGHPV NFSDVTMEQA RRDVEQLEQL IDNHDVIFLL MDTRESRWLP TVIAASKRKL VINAALGFDT FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG YKLGCYFCND VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSPKVLD QYEREGFTFL AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Plays also a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner.
  • 基因功能参考文献：
    1. Atg7(f/f) Tyr-Cre mice, in which autophagy-related 7 (Atg7) is conditionally deleted under the control of the tyrosinase promoter, are a model for accumulations of the autophagy adapter and substrate sequestosome-1/p62 in both neuronal and neuroepithelial cells. PMID: 29550918
    2. our study demonstrated that miR-143 mediates oxidative stress-induced autophagy to enhance the survival of c-kit(+) CPCs by targeting Atg7, which will provide a complementary approach for improving cardiac progenitor cells (CPCs)-based heart repair PMID: 29858017
    3. Atg5- and Atg7-dependent autophagy of dopaminergic neurons contributed to cellular and behavioral responses to morphine and may have implications for the future treatment of drug addiction. PMID: 28722508
    4. Deletion Atg7 in pancreatic acinar cells was sufficient to induce strong acinar cell death associated with acute pancreatitis that later progressed to chronic pancreatitis. PMID: 28703808
    5. Atg7 regulates IL-6 production via NF-kappaB to modulate brain angiogenesis. These findings established Atg7 as a novel regulatory molecule contributing to angiogenesis in the brain. PMID: 28467355
    6. Autophagy deficiency induced by RPE-specific deletion of Atg5 or Atg7 predisposes but does not necessarily drive the development of AMD-like phenotypes or retinal degeneration. PMID: 28465655
    7. ATG7 has a role in autophagy deficiency in macrophages that may contribute to the progression of metabolic syndrome associated with lipid injury and colitis PMID: 27337687
    8. ATG proteins ATG5 and ATG7 may be required for phagosome maturation under some conditions, but are not universally required for this process PMID: 27310610
    9. Autophagy and tumorigenesis were not increased in Atg7(+/-) mice kept in social isolation and fed OID. Thus, social isolation may increase breast cancer risk by inducing autophagy, independent of changes in body weight. PMID: 27550962
    10. ATG7, but not ATG13 or ULK1 has a role in functional autophagy in glioblastoma development PMID: 27304681
    11. knockdown of ATG7 results in decreased glycolysis and increased flux of labeled carbons through the mitochondrial tricarboxylic acid cycle. PMID: 27168493
    12. Atg7 is critical for the survival of midbrain dopaminergic (mDA) neurons in physiological condition. Atg7 is up-regulated when exposed to MPTP, but unlike in the control mice, chronic MPTP treatment did not lead to loss of mDA neurons in Atg7 conditional knockout mice. These results suggest that excessive Atg7-involved autophagy in the pathological condition has deleterious effects on the survival of mDA neurons. PMID: 27693472
    13. It shows that AMBRA1, but not ATG7, plays a role in TCR-mediated control of glycolytic factors and mitochondrial mass, while both AMBRA1 and ATG7 are required for autolysosome formation. PMID: 28789945
    14. Collectively, these data reveal that miR-20a inhibits autophagic response and promotes BCG survival in macrophages by targeting ATG7 and ATG16L1. PMID: 27803889
    15. Beta-Atg7 (-/-) mice had proinsulin-containing sequestosome 1 (p62 [also known as SQSTM1])(+) aggregates in beta cells, indicating proinsulin is regulated by autophagy in vivo. Short-term bafilomycin-A1 treatment and ATG5/7 knockdown increased steady-state proinsulin and hormone precursor chromogranin A content. ATG5/7 knockdown also increased glucose- and non-fuel-stimulated insulin secretion. PMID: 26831301
    16. The U2AF35(S34F) mutation alters interaction with CFIm59, leading to increased use of a distal cleavage and polyadenylation site in the ATG7 pre-mRNA, decreasing levels of ATG7 protein and defective autophagy, ultimately leading to transformation. PMID: 27184077
    17. gene deletion results in accumulation of RPE65 variant M450 in the retinal pigment epithelium PMID: 27537685
    18. A conditional deletion of Atg7 in hepatocytes leads to hepatomegaly and in aged animals to liver tumors. PMID: 27553638
    19. this study shows that knockdown of ATG7 enhances macrophage-associated phagocytosis and intracellular killing of Pseudomonas aeruginosa PMID: 27295610
    20. specific ablation of autophagy gene 7 (Atg7) from kidney proximal tubules worsened Lipopolysaccharide-induced acute kidney injury. PMID: 26916346
    21. These findings reveal that selective neuronal deletion of Atg7 is strongly protective against neuronal death. PMID: 26727396
    22. The canonical formation of autophagolysosomes was induced in demyelinating Schwann cells after injury, and the inhibition of autophagy via Schwann cell-specific knockout of the atg7 gene. PMID: 26712109
    23. Atg5 or Atg7 deletion in the chondrocytes promotes caspase-dependent cell death and leads to mild growth retardation PMID: 26077727
    24. atg7 knockout in mice or Atg7 knockdown in cell culture augmented ConA-induced acute hepatitis and related cellular malfunction, indicating protective effects of Atg7 on regulating mitochondrial ROS via a p38/MAPK-mediated pathway PMID: 26939081
    25. Treg cell-specific deletion of Atg7 leads to loss of Treg cells, greater tumor resistance and development of inflammatory disorders. Atg7-deficient Treg cells show increased apoptosis and readily lose expression of the transcription factor Foxp3. PMID: 26808230
    26. Atg7 deficiency produced an autophagy-deficient phenotype and delayed Pten-deficient prostate tumor progression in both castrate-naive and castrate-resistant cancers PMID: 26883359
    27. autophagy downregulates A20 in F4/80hi macrophages through p62-associated autophagic sequestration and that the resulting NFkappaB activation induces expression of chemokines at an early stage of Candida infection PMID: 25609235
    28. These observations indicate that Atg7-mediated autophagy is dispensable for retinoid recycling and A2E deposition; however, autophagy plays a role in coping with stress caused by A2E accumulation. PMID: 26468292
    29. ATG7 loss leads to endoplasmic reticulum stress, accumulation of dysfunctional mitochondria, oxidative stress, activation of AMPK, a decrease in autophagic flux, and a marked decrease in protein synthetic capacity that is accompanied by loss of rough ER. PMID: 26512112
    30. Studied the molecular mechanism by which Atg7 influenced K. pneumoniae-induced inflammatory responses. PMID: 26022442
    31. APF long noncoding RNA can regulate autophagic cell death by targeting miR-188-3p and ATG7. PMID: 25858075
    32. Atg7 Overcomes Senescence and Promotes Growth of BrafV600E-Driven Melanoma PMID: 25673642
    33. Inhibition of Atg7-mediated autophagy within the epithelium may prevent the development and progression of colorectal cancer in genetically predisposed patients. PMID: 26214133
    34. Data suggest that blocking autophagy in liver by deletion of Atg7 gene, which is essential for autophagosome formation, causes an increase in sphingolipid metabolites, including ceramide, in liver. PMID: 25332431
    35. Atg7 influences the transport of Abeta possibly derived from Golgi to multivesicular bodies. PMID: 25433221
    36. Germ cell-specific knockout of an essential autophagy induction gene Atg7 led to subfertility in female mice. PMID: 25590799
    37. Atg7 deficiency in myeloid cells causes mice to spontaneously develop pulmonary inflammation. PMID: 25911758
    38. Mutant mice developed mild podocyte and tubular dysfunction within 2 months, profound glomerular and tubular changes bearing close similarity to human disease by 4 months, and organ failure by 6 months. PMID: 25406339
    39. Atg7 enhances host defense against infection via downregulation of superoxide but upregulation of nitric oxide. PMID: 25535282
    40. results uncover a new role for Atg7 in the biogenesis of the acrosome, and provide evidence to support the autolysosome origination hypothesis for the acrosome PMID: 24853953
    41. Loss of autophagy-related gene 7 (Atg7) in dendritic cells ameliorates experimental autoimmune encephalomyelitis. PMID: 25077962
    42. Atg7 deficiency impairs host defense against Klebsiella pneumoniae by impacting bacterial clearance, survival and inflammatory responses PMID: 24993132
    43. Deleting Atg7 or Atg5 or blocking microtubule-associated protein 1 light chain (LC)3 lipidation or ATG5-ATG12 conjugation decreases ERK phosphorylation. PMID: 24240988
    44. The essential autophagy gene Atg7 functions to promote BrafV600E-driven lung tumorigenesis by preserving mitochondrial glutamine metabolism. PMID: 23965987
    45. Data indicate that human ATG16L1 and mouse autophagy components Atg7 and Atg16L1 participate in the IFN-gamma-induced recruitment of the immunity-related GTPases to the intracellular pathogen. PMID: 24563254
    46. Similar genetic interactions between Atg7 and Pten were observed in the context of DA turnover and DA-dependent locomotor behaviors. PMID: 24098148
    47. Atg7-dependent autophagy is active in differentiating epidermal keratinocytes but is not neccessary for establishing a functional skin barrier. PMID: 23669018
    48. F-actin is disassembled in Atg7-deficient mouse embryonic fibroblasts, indicating that depolymerization of F-actin impairs autophagosome maturation and that the intact F-actin network is required for basal and starvation-induced autophagy. PMID: 23850690
    49. ATG (BECN1 or ATG7) knockdown compromised mammary epithelial cell-mediated apoptotic body clearance in association with decreased RAC1 activation. PMID: 23380905
    50. Here we describe mice deficient in an essential macroautophagy component, Atg7, specifically within postnatal central nervous system neurons PMID: 22998728

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  • 亚细胞定位：
    Cytoplasm. Preautophagosomal structure.
  • 蛋白家族：
    ATG7 family
  • 组织特异性：
    Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
  • 数据库链接：

    KEGG: mmu:74244

    STRING: 10090.ENSMUSP00000032457

    UniGene: Mm.275332