Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Mouse Vesicular glutamate transporter 2 (Slc17a6), partial

Recombinant Mouse Vesicular glutamate transporter 2 (Slc17a6), partial

  • 货号：
    CSB-YP806867MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806867MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806867MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806867MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806867MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Slc17a6; Dnpi; Vglut2; Vesicular glutamate transporter 2; VGluT2; Differentiation-associated BNPI; Differentiation-associated Na(+)-dependent inorganic phosphate cotransporter; Solute carrier family 17 member 6
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Involved in the regulation of retinal hyaloid vessel regression during postnatal development.
  • 基因功能参考文献：
    1. the essential role of supraspinal VGLUT2 in neuropathic pain PMID: 29605418
    2. suggest that VGluT2 and GLT-1 may be differentially involved in the pathogenesis of amyotrophic lateral sclerosis via abnormal glutamate homeostasis at the presymptomatic stage and end stage of disease, respectively PMID: 28526579
    3. Vglut2 conditional knockout mice showed less interest in fatty rewards, decreased sugar consumption and strong rearing behavior, altered dopaminergic and peptidergic activity in the striatum. Significant cell loss and disorganization of the subthalamic nucleus structure was identified, which likely accounts for the observed alterations. PMID: 27699212
    4. depolarization-induced hyperacidification is mediated by the vesicular glutamate transporter 2 (VGLUT). PMID: 28823729
    5. The observed changes in gene and protein expression of vesicular transporter 1, regional architecture, and morphology of mouse auditory forebrain may relate to-and to some extent enable-the emergence of mature sound-evoked activity patterns. PMID: 26159773
    6. The data of this study indicated that the Grpr-Cre spinal cord neural population is composed of interneurons that use VGLUT2-mediated signaling for transmitting chemical and spontaneous itch stimuli to the next, currently unknown, neurons in the labeled line of itch. PMID: 28157737
    7. Study detected increased expression levels of the vesicular glutamate transporter 2 in motor cortex Layer V of G93A (Amyotrophic lateral sclerosis) mice. PMID: 25596588
    8. Male, but not female, mice lacking vGlut2 in POMC neurons were unable to maintain energy balance to the same extent as control mice. PMID: 26361382
    9. These results demonstrate that changes in the expression of supraspinal VGLUT2 may be a new mechanism relevant to the induction of neuropathic pain after nerve injury that acts through an aggravation of glutamate imbalance. PMID: 26300222
    10. Results suggest that both embryonal and adolescent forebrain expression of Vglut2 in mice normally contributes to balancing the level of anxiety. PMID: 25857802
    11. Data shows that VGLUT2-mediated glutamatergic transmission from Trpv1-Cre neurons selectively mediates heat and mechanical hypersensitivity associated with nerve injury. PMID: 25615623
    12. This study showed the the Vglut2 gene expression within all three VTA-VGLUT2 populations identified in the medial aspects of the A10 area and related to spatial memory. PMID: 24802380
    13. Loss og Vglut2 is associated with neonatal photoaversion but not adult pupillary light reflex. PMID: 24391855
    14. Vglut2-expressing cells in the subthalamic nucleus regulate dopaminergic transmission. PMID: 24821804
    15. VGLUT2 was found to be co-localized with muscarinic receptors on intraganglionic laminar endings of esophagus. PMID: 23742744
    16. VGLUT2 expression is pivotal to the proper development of mature pyramidal neuronal architecture and plasticity, and that such glutamatergic deficiency leads to cognitive malfunction PMID: 23136427
    17. VGLUT2 is a major mediator of nociception in primary afferents, implying that glutamate is the key somatosensory neurotransmitter. PMID: 22633683
    18. The spatial arrangement of VGluT2-IR and CGRP-IR terminals suggest that lamina I neurons receive convergent inputs from presumptive nociceptors that are primarily glutamatergic or peptidergic. PMID: 22152428
    19. This study demonstrates that absence of VGLUT2 in DA neurons leads to perturbations of reward consumption as well as reward-associated memory, features of particular relevance for addictive-like behavior. PMID: 21880920
    20. Data demonstrate that protein expression of VGLUT2 decreases age-dependently in senescence-accelerated mouse/prone 8 mice and increases in a learning- and memory-dependent manner in KM mice. PMID: 21295146
    21. A sensory subpopulation depends on vesicular glutamate transporter 2 for mechanical pain, and together with substance P, inflammatory pain. PMID: 21415372
    22. that VGLUT2 expression in nociceptors is essential for normal perception of acute pain and heat hyperalgesia PMID: 21135246
    23. This study establishes that vesicular glutamate transporter 2 is a major player in transient receptor potential cation channel subfamily V member 1 thermal nociception and also serves to regulate a normal itch response PMID: 21040852
    24. Elimination of VGLUT2 in nociceptors creates a mouse model of chronic neurogenic itch PMID: 21040853
    25. The results of this study provided definitive physiological evidence for VGLUT2-mediated glutamate release by mature dopamine neurons projecting to the NAc shell, but not to the dorsal striatum. PMID: 20554874
    26. analysis of genetic inactivation of the vesicular glutamate transporter 2 (VGLUT2) in the mouse [review] PMID: 20187846
    27. Results show that VGLUT2 expression in dopamine neurons is required for normal emotional reactivity as well as for psychostimulant-mediated behavioral activation. PMID: 20018672
    28. results suggest that reduced levels of VGLUT2 decrease motor neuron degeneration but do not prevent loss of motor neuron function in the SOD1(G93A) mouse model for amyotrophic lateral sclerosis PMID: 19770042
    29. Data show that MCH blocks the excitatory effect of kisspeptin on vGluT2-GnRH neurons. PMID: 19805188
    30. VGLUT2 is found in vagal afferent endings in the esophagus, suggesting that glutamate is contained in, and probably released from, synaptic vesicles previously described in intraganglionic laminar endings PMID: 12698357
    31. Subtype switching of vesicular glutamate transporters at parallel fibre-Purkinje cell synapses in developing mouse cerebellum. PMID: 12823463
    32. Mossy terminals of unipolar brush cells are immunoreactive for VGLUT2; moreover, dendritic brushes are contacted by mossy terminals expressing VGLUT2 in organotypic cultures of mouse vestibulocerebellum. PMID: 14614902
    33. developing hippocampal neurons transiently coexpressed VGLUT2 and VGLUT1 at distinct synaptic sites with different short-term plasticity PMID: 15118123
    34. results indicate VGluT2-loaded glutamatergic axons mature earlier than VGluT1-laden axons in the telencephalic & diencephalic regions & suggest VGluT1 plays a transient developmental role in some glutamatergic systems that mainly use VGluT2 in adulthood PMID: 16217795
    35. Mouse retinas were immunostained for vGluT1 and vGluT2. vGluT1 was localized to all cone pedicles and rod spherules, whereas vGluT2 was found in only 10% of the cone pedicles; which were distributed in a regular array, suggesting they are a distinct type. PMID: 16572432
    36. Vesicular VGLUT content is critically important for quantal size and VGLUT2-mediated reduction of excitatory drive affects specific forms of sensory processing. PMID: 17108179
    37. about 12% of the total number of dorsal root ganglia neuron profiles expressed VGLUT1 PMID: 17577523
    38. Cajal-Retzius cells and subplate neurons are glutamatergic; these two cells express VGLUT1 and VGLUT2, respectively, as the transport system of glutamate in events orchestrated by these cells during cortical development. PMID: 17651422
    39. VGluT2 is only found at terminals made by axons originating in the spinal cord and brainstem. By contrast, the two VGluT isoforms are co-localized in thalamocortical axon terminals targeting layer IV, but not in those targeting layer I. PMID: 18181146
    40. Cloned the mouse VGLUT2 promoter and showed a novel molecular mechanism of glucose-induced mVGLUT2 transcription involving Sp1. PMID: 18440316
    41. VGluT2 is the only vesicular glutamate transporter expressed in ventral tegmental area/substantia nigra dopamine neurons in vivo. PMID: 18562601
    42. vGLUT2 is likely involved in the epileptogenesis of generalized seizures PMID: 19171171
    43. Gonadotropin inhibitory hormone inhibits basal forebrain vGluT2-gonadotropin-releasing hormone neurons via a direct postsynaptic mechanism. PMID: 19204051
    44. The present study provides support for the existence of a neurocircuitry that connects changes in VGLUT2-mediated neurotransmission to alterations in the dopaminergic system with schizophrenia-like behavioral deficits as a major outcome. PMID: 19228977

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
  • 蛋白家族：
    Major facilitator superfamily, Sodium/anion cotransporter family, VGLUT subfamily
  • 组织特异性：
    Expressed in brain. Expressed in hippocampal neurons (at protein level).
  • 数据库链接：

    KEGG: mmu:140919

    STRING: 10090.ENSMUSP00000032710

    UniGene: Mm.256618