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  4. Recombinant Mouse Villin-1 (Vil1)

Recombinant Mouse Villin-1 (Vil1)

  • 中文名称：
    小鼠Vil1重组蛋白
  • 货号：
    CSB-YP717266MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Vil1重组蛋白
  • 货号：
    CSB-EP717266MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Vil1重组蛋白
  • 货号：
    CSB-EP717266MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Vil1重组蛋白
  • 货号：
    CSB-BP717266MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Vil1重组蛋白
  • 货号：
    CSB-MP717266MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Vil1
  • Uniprot No.：
  • 别名：
    Vil1; VilVillin-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination.
  • 基因功能参考文献：
    1. Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
    2. This study also highlights the potential role of villin's pro-apoptotic function in the pathogenesis of inflammatory bowel disease, ischemia-reperfusion injury, enteroinvasive bacterial and parasitic infections. PMID: 27765954
    3. Competition between tryptophan fluorescence and electron transfer during unfolding of the villin headpiece. PMID: 24972184
    4. villin severs F-actin to ensure microvillus depolarization and enterocyte remodeling upon intestinal injury PMID: 23520048
    5. Villin-Cre(+);Klf4(fl/fl) have greater susceptibility to chemical-induced gastric carcinogenesis and increased rates of gastric tumor progression than control mice. PMID: 22155367
    6. The data establish that meprins interact with and cleave villin and actin, and these cytoskeletal proteins are substrates for meprins. PMID: 21795642
    7. a lack of villin does not significantly alter basal and secretagogue-stimulated electrolyte movements across the epithelium of the mouse jejunum in vivo PMID: 11897622
    8. These results indicate that an important patterning event involving villin expression occurs in the gut epithelium at 16 days, which may define the epithelial boundary between stomach and intestine. PMID: 11984877
    9. different regulatory sequences of the villin gene regulate expression in the intestinal epithelium PMID: 12065599
    10. Data show that villin, characteristic markers of brush borders, become abnormally distributed along these atypical basolateral membranes in gal3(-/-) mice. PMID: 18211959
    11. Villin is an actin regulatory protein that organizes, integrates and regulates multiple epithelial cell functions. PMID: 18307996

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, microvillus. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, filopodium tip. Cell projection, filopodium.
  • 蛋白家族：
    Villin/gelsolin family
  • 组织特异性：
    Expressed in small intestin, colon, kidney and enterocytes (at protein level).
  • 数据库链接：

    KEGG: mmu:22349

    STRING: 10090.ENSMUSP00000027366

    UniGene: Mm.471601