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  4. Recombinant Mouse Visual system homeobox 2 (Vsx2)

Recombinant Mouse Visual system homeobox 2 (Vsx2)

  • 货号：
    CSB-YP025939MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025939MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025939MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025939MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025939MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Vsx2
  • Uniprot No.：
  • 别名：
    Vsx2; Chx10; Visual system homeobox 2; Ceh-10 homeodomain-containing homolog; Homeobox protein CHX10
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-361
  • 氨基酸序列
    MTGKAGEALS KPKSETVAKS TSGGAPARCT GFGIQEILGL NKEPPSSHPR AALDGLAPGH LLAARSVLSP AGVGSMGLLG PGGLPGFYTQ PTFLEVLSDP QSVHLQPLGR ASGPLDTSQT ASSDSEDVSS SDRKMSKSAL NQTKKRKKRR HRTIFTSYQL EELEKAFNEA HYPDVYAREM LAMKTELPED RIQVWFQNRR AKWRKREKCW GRSSVMAEYG LYGAMVRHSI PLPESILKSA KDGIMDSCAP WLLGMHKKSL EAAAESGRKP EVERQALPKL DKMEQEERAP EAQAAISQEE LRENSIAALR AKAQEHSTKV LGTVSGPDSL ARNAEKPEEE DATEEDRPAE KLSPPQLEDM A
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a transcriptional regulator through binding to DNA at the consensus sequence 5'-[TC]TAATT[AG][AG]-3' upstream of gene promoters. Plays a significant role in the specification and morphogenesis of the sensory retina. Mediates differentiation of V2a interneurons by repression of motor neuron gene transcription, via competitively binding to response elements that are activated by the ISL1-LHX3 complex, such as VSX1. Acts as a positive transcriptional regulator of NXNL1; regulation is significantly increased in synergy with VSX1. Acts as a negative transcriptional regulator of MITF. Represses SAG transcription by competitive inhibition of ISL1-LHX3 response elements. Binds to the photoreceptor conserved element-1 (PCE-1) in the promoter of rod photoreceptor arrestin SAG and acts as a transcriptional repressor. Involved in the development of retinal ganglion cells (RGCs) which leads to release of SHH by RGCs, promoting Hedgehog signaling and subsequent proliferation of retinal progenitor cells. Participates in the development of the cells of the inner nuclear layer, by promoting postnatal differentiation of bipolar cells with a comparable inhibition of rod cell differentiation. May play a role in the maintenance of neural retina identity during development by regulation of canonical Wnt genes and CTNNB1 localization, suggesting a role in the regulation of canonical Wnt signaling.
  • 基因功能参考文献：
    1. Vsx2 represses Mitf in a cell-autonomous way. It also uses cell-nonautonomous mechanisms to regulate progenitor properties in the embryonic retina. Vsx2's role in regulating Mitf is in part separable from its role in promoting proliferation. PMID: 25927996
    2. The results of this study suggested that Lhx3/Chx10 medullary reticular formation neurons are involved in locomotion. PMID: 24027269
    3. Our data suggest that the phenotypic severity of the CVC mutant depends on the weakened DNA binding activity elicited by the CVC mutation and a previously unknown protein interaction between Vsx2 and its regulatory target Mitf. PMID: 23028343
    4. analysis of the regulatory role of a conserved motif adjacent to the homeodomain of Hox10 proteins PMID: 22721778
    5. Reducing GDF11 levels might promote restoration of retinal development in the microophthalmic Vsx2 mutant mouse. PMID: 22183392
    6. Data demonstrate that Hox10 genes play a critical role in the developing kidney. PMID: 21858105
    7. Blimp1 may play a role in photoreceptor development by repressing genes involved in bipolar cell fate specification and retinal cell proliferation in differentiating precursors; Blimp1 can bind to the Chx10 enhancer and repress Chx10 enhancer activity PMID: 20463215
    8. CHX10 regulates RdCVF promoter activity in the inner retina. PMID: 19843539
    9. results have shown that the Hox10 and Hox11 paralogous genes are global regulators of the lumbosacral region of the axial skeleton and are integral in patterning principal limb elements PMID: 12869760
    10. Delay of the normal temporal expression of genes essential for photoreceptor disc morphogenesis leads to failure of correct rod and cone outer segment formation in the Chx10(or-J/or-J PMID: 14744875
    11. linked to maintenance of the neural retina PMID: 15459106
    12. repression of Mitf by FGF is Chx10 dependent, indicating that FGF, Chx10 and Mitf are components of a pathway that determines and maintains the identity of the neuroretina PMID: 15576400
    13. CHX10 may target specific motifs to inhibit rod photoreceptor gene expression in bipolar cells PMID: 16236706
    14. Chx10 directly controls bipolar cell genesis by inhibiting rod differentiation independent of its temporally limited early effect on retinal progenitor cell proliferation PMID: 16547132
    15. Scan reveals significant loci on chromosomes 6 and 14, where the CASA/Rk alleles of Chx10 from a backcross between 129/SvJ and Mus musculus castaneus are maintained selectively and show a much milder Chx10 phenotype. PMID: 16783634
    16. Although Vsx1 mRNA is upregulated in Chx10/Vsx2 deficient RPCs, Vsx1 does not genetically compensate for loss of Chx10/Vsx2, demonstrating that Prd-L:CVC genes, although important, are not absolutely required to initiate retinal development. PMID: 17919464
    17. Vsx2/Chx10 ensures the correct timing and magnitude of Hedgehog signaling in the mouse retina. PMID: 18417110
    18. Pax6 interacts with Chx10 via its paired domain or homeodomain (HD) and the HD of Chx10, resulting in enhanced transactivation by Pax6. This interaction was modeled on a demonstrated interaction between zebrafish Pax6 and mouse Chx10. PMID: 11069920

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  • 相关疾病：
    Defects in Vsx2 are the cause of ocular retardation (OR(J)), a mouse disease characterized by microphthalmia, progressive destruction of the retina, and absence of the optic nerve. The OR(J) mutation is due to an OCHR (STOP) mutation.
  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Paired homeobox family
  • 组织特异性：
    Expressed in the retina (at protein level). Expressed throughout the anterior optic vesicle and all neuroblasts of the optic cup (at protein level). In the mature and postnatal retina is restricted to the inner nuclear layer, in which its expression decre
  • 数据库链接：

    KEGG: mmu:12677

    STRING: 10090.ENSMUSP00000021665

    UniGene: Mm.4405