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  4. Recombinant Mouse WAS protein family homolog 1 (Wash1)

Recombinant Mouse WAS protein family homolog 1 (Wash1)

  • 货号：
    CSB-YP025972MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP025972MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP025972MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP025972MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP025972MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Washc1; Orf19; Wash; Wash1WASH complex subunit 1; WAS protein family homolog 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-475
  • 氨基酸序列
    MTPVKTQCSL AGQLYAVPLI QPDLRREEAI QQVADALQYL QNISGDIFSR ISRRVELSRR QLQAISERVS LAQAKIEKIK GSKKAIKVFS SAKYPAPEHL QEYGSIFTGA LDPGLQRRPR YRIQSKHRPL DERALQEKLK YFPVCVNTKS EPEDEAEEGL GGLPSNISSI SSLLLFNTTE NLYKKYVFLD PLAGAVTKTH TMLGTEEEKL FDAPLSISKR EQLERQAPEN YFYVPDLGQV PEIDVPSYLP DLPGVADDLM YSADLGPGIA PSAPGAIPEL PAFHTEVAEP LQPELENEVL LAAPPPPPPP PPPPPPAPTA LVSTPQPPMF PDMATAAGQV AREEDSSSSM AHTASVQGAP KEVVDPSSGR ATLLESIRQA GGIGKAKLRS VKERKLEKKK QKEQEQVRAT SQGGDLMSDL FNKLVMRRKG ISGKGPSTGT SEGPGGAFSR MSDSIPPLPP PQQPAGDEDE EDWES
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T-cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in cytokinesis and following polar body extrusion during oocyte meiotic maturation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.
  • 基因功能参考文献：
    1. WASH deficiency impairs the association of RNF2 with AMBRA1 to impede AMBRA1 degradation. PMID: 24980959
    2. WASH complex regulates Arp2/3 complex and is required for cytokinesis and polar body extrusion. PMID: 24998208
    3. WASH is required for the differentiation commitment of hematopoietic stem cells by acting as an upstream regulator to modulate c-Myc transcription. PMID: 25225459
    4. WASH can suppress Beclin 1 ubiquitination to inactivate Vps34 activity leading to suppression of autophagy. PMID: 23974797
    5. WASH is a newly recognized regulator of T cell receptor, CD28, LFA-1, and GLUT1 endosome-to-membrane recycling. PMID: 23275443
    6. WASH-mediated F-actin is requisite for the integrity of both endosomal and lysosomal networks in mammalian cells. PMID: 22718907
    7. WASH is a bimodular protein and a component of the BLOC-1 complex in which the C terminus is involved in Arp2/3-mediated actin nucleation, whereas the N-terminal portion is required for its regulation and localization in the cells PMID: 20308062
    8. show that WASH generates an actin network on a restricted domain of sorting and recycling endosomes. PMID: 19922875

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  • 亚细胞定位：
    Early endosome membrane. Recycling endosome membrane. Cytoplasmic vesicle, autophagosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole.
  • 蛋白家族：
    WASH1 family
  • 组织特异性：
    Ubiquitously expressed.
  • 数据库链接：

    KEGG: mmu:68767

    STRING: 10090.ENSMUSP00000072220

    UniGene: Mm.296545