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  4. Recombinant Mouse Wiskott-Aldrich syndrome protein family member 2 (Wasf2)

Recombinant Mouse Wiskott-Aldrich syndrome protein family member 2 (Wasf2)

  • 中文名称：
    小鼠Wasf2重组蛋白
  • 货号：
    CSB-YP806368MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Wasf2重组蛋白
  • 货号：
    CSB-EP806368MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Wasf2重组蛋白
  • 货号：
    CSB-EP806368MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Wasf2重组蛋白
  • 货号：
    CSB-BP806368MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Wasf2重组蛋白
  • 货号：
    CSB-MP806368MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Wasf2; Wave2; Wiskott-Aldrich syndrome protein family member 2; WASP family protein member 2; Protein WAVE-2
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-497
  • 氨基酸序列
    MPLVTRNIEP RHLCRQTLPS DTSELECRTN ITLANVIRQL GSLSKYAEDI FGEICTQASA FASRVNSLAE RVDRVQVKVT QLDPKEEEVS LQGINTRKAF RSSTTQDQKL FDRNSLPVPV LETYNSCDAP PPLNNLSPYR DDGKEALKFY TNPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK EEWEKMKMGQ EFVESKERLG PSGYSSTLVY QNGSIGSVEN VDAASYPPPP QSDSASSPSP SFSEDNLPPP PAEFSYPADN QRGSVLAGPK RTSMVSPSHP PPAPPLSSPP GPKPGFAPPP APPPPPPMSV PPPLPSMGFG SPGTPPPPSP PSFPPHPDFA APPPPPPPPA ADYPMPPPPL SQPSGGAPPP PPPPPPPGPP PLPFSGADGQ PAAPPPPPPS EATKPKSSLP AVSDARSDLL SAIRQGFQLR RVEEQREQEK RDVVGNDVAT ILSRRIAVEY SDSEDDSSEF DEDDWSD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
  • 基因功能参考文献：
    1. WAVE2/c-Abl survival signal required at the hematopoietic stem cell (HSC) fetal liver to marrow transition that confers fitness to the HSC for marrow hematopoiesis PMID: 29915352
    2. TrkC signals to the podocyte actin cytoskeleton to induce migration by phosphorylating WAVE2 Erk dependently. This signaling mechanism may be important for TrkC-mediated cytoskeletal dynamics in podocyte disease. PMID: 29162704
    3. Rho family GTPases use the I-BAR proteins, IRSp53 (also known as BAIAP2), IRTKS and Pinkbar, as a central mechanism to modulate cell morphology. PMID: 27278019
    4. Data show that the primary effect of costimulation blockade was to decrease recruitment of the activator of actin nucleation WAVE2 (Wiskott-Aldrich syndrome protein family member protein 2) and cofilin (actin-severing protein) to F-actin. PMID: 27095595
    5. The expression of NESH/Abi-3 caused degradation of endogenous Abi-1, which led to the formation of a NESH/Abi-3-based WAVE2 complex. PMID: 26428302
    6. This study demonstrated that WAVE2-Abi2 complex, which controls growth cone activity, is crucial for completing transition from a multipolar to bipolar shape. PMID: 22617848
    7. Results of the present study show that the nucleation-promoting factors JMY and WAVE2 are critical for cytokinesis during development of mouse embryos. PMID: 23552571
    8. These results identify Wasf2 as a novel target of intermittent PTH administration via the Wnt and phosphoinositide-dependent protein kinase signaling pathways. PMID: 23525371
    9. The protein and messenger RNA levels of WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes. PMID: 22306711
    10. WAVE2 regulates oocyte polarization by regulating meiotic spindle, peripheral positioning, probably via an actin-mediated pathway, and is involved in polar body emission during mouse oocyte meiotic maturation. PMID: 21543895
    11. sential role for Abi1 in embryonic survival and WAVE2 complex integrity PMID: 21482783
    12. WAVE2 works as an A-kinase-anchoring protein that recruits PKA at membrane protrusions and plays a role in the enlargement of membrane protrusions induced by PKA activation PMID: 21119216
    13. WAVE2 colocalised with IRSp53 at the tips of protruding lamellipodia and filopodia, and the IRSp53 signal in filopodia decreased before that of WAVE2 during retraction. PMID: 12734400
    14. WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility. PMID: 12853475
    15. WAVE2-regulated actin reorganization might be required for proper cell movement and that a lack of functional WAVE2 impairs angiogenesis in vivo PMID: 12879075
    16. Results suggest that phosphatidylinositol (3,4,5)-triphosphate recruits WAVE2 to the polarized membrane and that this recruitment is essential for lamellipodium formation at the leading edge. PMID: 15107862
    17. WAVE2 acts as the primary effector downstream of Rac to achieve invasion and metastasis, suggesting that suppression of WAVE2 activity holds a promise for preventing cancer invasion and metastasis. PMID: 15608687
    18. Altogether these data identify a WAVE2-Abi1 complex crucial for the normal actin cytoskeleton reorganization and migration of macrophages in response to CSF-1. PMID: 16280551
    19. c-Abl activates WAVE2 via tyrosine phosphorylation to promote actin remodeling in vivo; Abi-1 forms the crucial link between these two factors PMID: 16899465
    20. in vitro WAVE2 is directly phosphorylated by extracellular signal-regulated kinase (ERK) 2. The proline-rich region and the verprolin, cofilin and acidic (VCA) region of WAVE2 were phosphorylated. PMID: 17202194
    21. The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading. PMID: 17664349
    22. Phosphorylation of WAVE2 by MAP kinases regulates persistent cell migration and polarity. PMID: 18032787
    23. WAVE2-mediated actin polymerization, potentially downstream of Rac1, plays an important role in intramarrow mobilization and proliferation of hematopoietic stem cells. PMID: 19415782

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Basolateral cell membrane.
  • 蛋白家族：
    SCAR/WAVE family
  • 数据库链接：

    KEGG: mmu:242687

    STRING: 10090.ENSMUSP00000081263

    UniGene: Mm.23566