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  4. Recombinant Mouse Yorkie homolog (Yap1)

Recombinant Mouse Yorkie homolog (Yap1)

  • 中文名称：
    小鼠Yap1重组蛋白
  • 货号：
    CSB-YP026244MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Yap1重组蛋白
  • 货号：
    CSB-EP026244MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Yap1重组蛋白
  • 货号：
    CSB-EP026244MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Yap1重组蛋白
  • 货号：
    CSB-BP026244MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    小鼠Yap1重组蛋白
  • 货号：
    CSB-MP026244MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Yap1; Yap; Yap65; Transcriptional coactivator YAP1; Yes-associated protein 1; Protein yorkie homolog; Yes-associated protein YAP65 homolog
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-488
  • 氨基酸序列
    MEPAQQPPPQPAPQGPAPPSVSPAGTPAAPPAPPAGHQVVHVRGDSETDLEALFNAVMNP KTANVPQTVPMRLRKLPDSFFKPPEPKSHSRQASTDAGTAGALTPQHVRAHSSPASLQLG AVSPGTLTASGVVSGPAAAPAAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHNDQ TTTWQDPRKAMLSQLNVPAPASPAVPQTLMNSASGPLPDGWEQAMTQDGEVYYINHKNKT TSWLDPRLDPRFAMNQRITQSAPVKQPPPLAPQSPQGGVLGGGSSNQQQQIQLQQLQMEK ERLRLKQQELFRQAIRNINPSTANAPKCQELALRSQLPTLEQDGGTPNAVSSPGMSQELR TMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSIPRTPDDFLNSVDEMDTGDTISQSTLP SQQSRFPDYLEALPGTNVDLGTLEGDAMNIEGEELMPSLQEALSSEILDVESVLAATKLD KESFLTWL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization. Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1. In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation.
  • 基因功能参考文献：
    1. Expression of mutant GNAS caused phosphorylated YAP1 to be sequestered in the cytoplasm, altering tumor progression. PMID: 30142336
    2. Using liver cancer as a model, NUAK2 was identified as an essential mediator of YAP-driven hepatomegaly and tumorigenesis in vivo. PMID: 30446657
    3. YAP determines the cell fate of injured mouse hepatocytes in vivo. PMID: 28681838
    4. disruption of TAZ/YAP activity alleviates tumor burden in Lats1/2-deficient mice and inhibits human malignant peripheral nerve sheath tumors cell proliferation PMID: 29438698
    5. YAP1 and the YAP1-TEADs complex have roles in regulating osteoclastogenesis and related gene expression PMID: 29432919
    6. Inhibition of YAP ameliorates choroidal neovascularization via inhibiting endothelial cell proliferation. PMID: 29422766
    7. YAP/TAZ mechanotransduction integrates with cell-cell communication pathways for fine-grained orchestration of stem cell decisions. PMID: 28513598
    8. Nuclear expression of YAP1 is detected in a small subset of hepatic cells starting at embryonic day (E) 13.5 when the hepatoblasts begin to differentiate into hepatocytes and cholangiocytes. At E18.5, nuclear YAP1 is undetectable in hepatoblasts & hepatocytes, but enriched within the nuclei of cholangiocytes. These levels remain postnatally, consistent with the role of YAP1 in cholangiocyte specification and maintenance. PMID: 29627454
    9. Fat4 is not required for the canonical activation of Hippo kinases but it sequesters a partner of Yap1, Amotl1, out of the nucleus. PMID: 28239148
    10. Functional impairment of YAP/YAPdeltaC by mutant Atxn1 during development determines the adult pathology of spinocerebellar ataxia type 1 by suppressing RORalpha-mediated transcription. PMID: 29192206
    11. Low YAP expression is associated with low liver regeneration. PMID: 30142376
    12. these observations suggest that Zyxin promotes colon cancer tumorigenesis in a mitotic-phosphorylation-dependent manner and through CDK8-mediated YAP activation. PMID: 29967145
    13. During and after liver development, the activation of YAP/TAZ induced by loss of Lats1/2 forces hepatoblasts or hepatocytes to commit to the biliary epithelial cell lineage. PMID: 27358050
    14. YAP promotes myelin and non-myelin genes transcription while the polarity protein Crb3, localized at the tips of the myelin sheath, activates the Hippo pathway to temper YAP activity, therefore allowing for optimal myelin growth. PMID: 27435623
    15. RHOA loss reduces YAP signaling of the Hippo pathway and affects YAP effector epiregulin (EREG) expression in the crypts. Expression of an active YAP (S112A) mutant rescues ntestinal stem cells (ISCs) marker expression, ISC regeneration, and ISC-associated Wnt signaling, but not defective epithelial polarity, in RhoA knockout mice, implicating YAP in RHOA-regulated ISC function. PMID: 29129684
    16. YAP promotes the neurite outgrowth via targeting the promoter of miR-29a, and it may be an effective therapeutic medicine for the neural disease. PMID: 29138751
    17. Physiologically, steroid sex hormones stimulate follicle growth by activating YAP1; however, the preovulatory inhibition of YAP1 activity in granulosa cells is a prerequisite of LH actions. PMID: 28961951
    18. Hedgehog-YAP signaling pathway regulates glutaminolysis to control activation of hepatic stellate cells and their transdifferentiation into myofibroblasts. PMID: 29305935
    19. The role of Yap and Wwtr1 in the Hippo signaling pathway and the regulation of spermatogenesis in mice are reported. PMID: 28637242
    20. demonstrated the nuclear expression of transcriptional coactivator YAP1 in the limbal and corneal basal epithelial cells and its essential role for maintaining the high proliferative potential of those corneal epithelial progenitor cells in vivo PMID: 27734924
    21. Taz and Yap have overlapping functions in promoting myoblast proliferation but Taz then switches to enhance myogenic differentiation. PMID: 28589555
    22. identified a pathway involving activation of integrin alpha3 in TA cells that signals through an LATS-independent FAK/CDC42/PP1A cascade to control YAP-S397 phosphorylation and nuclear localization. PMID: 28457749
    23. thiazolidinediones (PPARgamma agonists) promote differentiation of cancer stem cells by restraining YAP transcriptional activity PMID: 27528232
    24. RASSF1A mediates transcription factor selection of YAP in stem cells.YAP induces OCT4 expression. PMID: 29382819
    25. Collectively, these data indicate that Yap-induced Epiregulin signaling promotes the identity of submandibular gland ductal progenitors and that removal of nuclear Yap by Lats1/2-mediated signaling is critical for proper ductal maturation. PMID: 28492365
    26. The s show that active YAP, a key mediator of Hippo signaling, is distributed throughout the murine lung epithelium and loss of epithelial YAP severely disrupts branching. Failure to branch is restricted to regions where YAP activity is removed. This suggests that YAP controls local epithelial cell properties. PMID: 28323616
    27. Collectively, our results defined netrin-1 as a positive regulator of malignant tumor metastasis in GC by activating the YAP signaling, with potential implications for new approaches to GC therapy. PMID: 29305865
    28. Decrease in actin tension and YAP inactivation should be crucially involved in the cytotoxicity of ethanol on HL-1 cardiomyocytes. PMID: 28904289
    29. The results of this study indicated that YAP regulates oligodendrocyte morphology and maturation in response to mechanical factors. PMID: 27807898
    30. Study found that the extracellular matrix protein laminin-511 (LM511) promoted the survival and differentiation of dopaminergic neurons in the midbrain neurons. LM511 bound to integrin alpha3beta1 and activated the transcriptional cofactor YAP. PMID: 28831020
    31. YAP accumulated in nuclei of mammary glands in ErbB2/EGFR-transgenic mice, suggesting that EGFR signaling affects YAP in vivo similar to cell culture. ErbB2/EGFR-transgenic mice develop mammary tumors in 7-8 months, but surprisingly, MaSCs from these mice did not form tumors when transplanted into host mice. PMID: 27601049
    32. Therefore, YAP/TAZ are crucial for Schwann cells to myelinate developing nerve and to maintain myelinated nerve in adulthood. PMID: 28124973
    33. Collectively, we have uncovered that AMOT acts as a YAP stimulator in high glucose level. PMID: 29217192
    34. YAP1 enhanced cementoblast mineralization in vitro. YAP1 exerted its effect on the cementoblast partly by regulating the Smad-dependent BMP and Erk1/2 signaling pathways. PMID: 28688217
    35. A crucial role for YAP and TAZ in the maintenance of the postnatal adrenal cortex. PMID: 28938438
    36. appears to reprogram cellular metabolism, diverting substrates away from the energy-consuming process of gluconeogenesis and toward the anabolic process of growth PMID: 28714135
    37. Dystrophin-glycoprotein complex component dystroglycan 1 (Dag1) directly binds to the Hippo pathway effector Yap to inhibit cardiomyocyte proliferation in mice PMID: 28581498
    38. P38 MAPK-mediated YAP activation controls mechanical-tension-induced pulmonary alveolar regeneration. PMID: 27498861
    39. YAP1 exerted its effect on the chondrocyte differentiation by activating the Wnt/beta-catenin signaling pathway PMID: 28438716
    40. Nuclear YAP does not play a significant physiological role during oocyte development in mammals. PMID: 26985001
    41. identify the mesenchymal requirement of YAP/TAZ in the gastrointestinal tract and highlight the functional interplays between Hippo and Hedgehog signaling underlying temporal and spatial control of tissue growth and specification in developing gut PMID: 28943241
    42. findings indicate that HIF-2alpha increases cancer cell growth by up-regulating YAP1 activity PMID: 28848049
    43. found that epidermal YAP activity drives GLI2 nuclear accumulation in the skin of YAP2-5SA-DeltaC mice, which depends on epidermal beta-catenin activation PMID: 28820907
    44. Yap and Taz leads to impaired epicardial epithelial-to-mesenchymal transition (EMT) and a reduction in epicardial cell proliferation and differentiation into coronary endothelial cells. PMID: 27160901
    45. These results suggest that YAP promotes muscle differentiation by activating the Abl/Src/MEKK3/MEK5/ERK5 kinase cascade. PMID: 28356344
    46. data demonstrate that Yap1 is a required factor for proper differentiation of mouse embryonic stem cells, while remaining dispensable for self-renewal PMID: 26917425
    47. these results identify a novel function of YAP in neocortical astrocytic differentiation and proliferation, and reveal a BMP2-YAP-SMAD1 pathway underlying astrocytic differentiation in the developing mouse neocortex. PMID: 27381227
    48. Epicardial YAP/TAZ orchestrate an immunosuppressive response following myocardial infarction PMID: 28165342
    49. YAP negatively regulated an antiviral immune response. YAP deficiency resulted in enhanced innate immunity, a diminished viral load, and morbidity in vivo. YAP blocked dimerization of the transcription factor IRF3 and impeded translocation of IRF3 to the nucleus after viral infection. PMID: 28481329
    50. the transcription regulators YAP and TAZ localise to the nucleus in the basal layer of skin and are elevated upon wound healing. PMID: 26989177

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 蛋白家族：
    YAP1 family
  • 组织特异性：
    Isoforms lacking the transactivation domain seen in striatal neurons (at protein level). Ubiquitous. Isoform 2 is expressed at higher levels in the neural tissues. In the embryo, it is expressed in brain, eye, and the maxillary and frontonasal components
  • 数据库链接：

    KEGG: mmu:22601

    STRING: 10090.ENSMUSP00000069554

    UniGene: Mm.221992