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  4. Recombinant Mouse Z-DNA-binding protein 1 (Zbp1)

Recombinant Mouse Z-DNA-binding protein 1 (Zbp1)

  • 中文名称：
    小鼠Zbp1重组蛋白
  • 货号：
    CSB-YP865587MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    小鼠Zbp1重组蛋白
  • 货号：
    CSB-EP865587MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    小鼠Zbp1重组蛋白
  • 货号：
    CSB-EP865587MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    小鼠Zbp1重组蛋白
  • 货号：
    CSB-BP865587MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Zbp1; Dlm1; Z-DNA-binding protein 1; DNA-dependent activator of IFN-regulatory factors; DAI; Tumor stroma and activated macrophage protein DLM-1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-411
  • 氨基酸序列
    MAEAPVDLST GDNLEQKILQ VLSDDGGPVK IGQLVKKCQV PKKTLNQVLY RLKKEDRVSS PEPATWSIGG AASGDGAPAI PENSSAQPSL DERILRFLEA NGPHRALHIA KALGMTTAKE VNPLLYSMRN KHLLSYDGQT WKIYHSRQEG QDIAHSGVTQ ESPAIICQHN PVNMICQQGA NSHISIANSN AIQIGHGNVI VREKACGEPG PRTSHPLPLA WDASAQDMPP VAHGAQYIYM DKSLLQQVQL GHHNEMSLVG DAGKHPSYSF SDSPPEVSTT TADPGASFNM QTFEPGPHPE GDTVQTVHIK SCFLEDATIG NGNKMTIHLR SKGEVMESGD SEEPKKEDTG TSSEATPPRS CQHTPSDSML PTSELRAMAL GDSSPQTTEP VLREHEVQDI ESSQDTGLSK Q
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key innate sensor that recognizes and binds Z-RNA structures, which are produced by a number of viruses, such as herpesvirus, orthomyxovirus or flavivirus, and triggers different forms of cell death. Once activated upon Z-RNA-binding, ZBP1 interacts with RIPK3, inducing the complementary pathways of apoptosis, necroptosis and pyroptosis. Acts as a key activator of necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members: ZBP1-dependent necroptosis involves RIPK3 stimulation, which phosphorylates and activates MLKL, triggering execution of programmed necrosis. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: ZBP1 recognizes and binds Z-RNA structures that are produced in infected nuclei by orthomyxoviruses, such as the influenza A virus (IAV), leading to ZBP1 activation, RIPK3 stimulation and subsequent MLKL phosphorylation, triggering disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. ZBP1-dependent cell death in response to IAV infection promotes interleukin-1 alpha (IL1A) induction in an NLRP3-inflammasome-independent manner: IL1A expression is required for the optimal interleukin-1 beta (IL1B) production, and together, these cytokines promote infiltration of inflammatory neutrophils to the lung, leading to the formation of neutrophil extracellular traps. In some cell types, also able to restrict viral replication by promoting cell death-independent responses. In response to flavivirus infection in neurons, promotes a cell death-independent pathway that restricts viral replication: together with RIPK3, promotes a death-independent transcriptional program that modifies the cellular metabolism via up-regulation expression of the enzyme ACOD1/IRG1 and production of the metabolite itaconate. Itaconate inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes.
  • 基因功能参考文献：
    1. PUMA promotes the cytosolic release of mitochondrial DNA and activation of the DNA sensors DAI/Zbp1 and STING, leading to enhanced RIP3 and MLKL phosphorylation in a positive feedback loop. PMID: 29581256
    2. Murine cytomegalovirus IE3-dependent transcription from the viral genome plays a crucial role in activating DAI/ZBP1-dependent necroptosis. This implicates RNA transcripts generated by a large double-stranded DNA virus as a biologically relevant ligand for DAI/ZBP1 during natural viral infection. PMID: 28607035
    3. activation requires RIG-I signaling, ubiquitination, and vRNP sensing to trigger activation of programmed cell death pathways during influenza A virus infection PMID: 28634194
    4. findings identify ZBP1 as a critical mediator of inflammation beyond its previously known role in antiviral defence and suggest that ZBP1 might be implicated in the pathogenesis of necroptosis-associated inflammatory diseases PMID: 27819681
    5. findings indicate that the RHIM of RIPK1 is critical for preventing ZBP1/RIPK3/MLKL-dependent necroptosis during development PMID: 27819682
    6. DAI as an important cytosolic DNA sensor, is responsible for the recognition of A/T or G/C-rich DNA derived from DNA virus. PMID: 26648291
    7. Disruption of the in vivo interaction of KIF11 with ZBP1 delocalizes beta-actin mRNA and affects cell migration. PMID: 25588836
    8. Association of ribosomes and ZBP1 with the mRNA were anti-correlated both near the nucleus of primary fibroblasts and neurons and in peripheral cellular regions such as the leading edge of fibroblasts, therefore validating the hypothesis that ZBP1 spatially regulates beta-actin mRNA localized translation. PMID: 26140598
    9. data support a direct role for ZBP1 in transport and translation of mRNA cargos in axonal regeneration in vitro and in vivo PMID: 21964071
    10. observations underscore an integral role of DAI (DLM-1/ZBP1) in the DNA-mediated activation of innate immune responses PMID: 17618271
    11. mechanism of DAI activation and the complex regulatory mechanisms underlying DNA-mediated protective and pathologic immune responses. PMID: 18375758
    12. shares common structure-specific recognition core within the binding domain with an RNA editing enzyme but binds Z-DNA PMID: 11524677

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Expressed in lung, spleen and liver. Lower levels were seen in heart, kidney and testis. Expression is greatly up-regulated in tumor stromal cells and activated macrophages.
  • 数据库链接：

    KEGG: mmu:58203

    STRING: 10090.ENSMUSP00000029018

    UniGene: Mm.116687