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  4. Recombinant Mouse Zinc finger protein Gfi-1 (Gfi1)

Recombinant Mouse Zinc finger protein Gfi-1 (Gfi1)

  • 货号：
    CSB-YP009371MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009371MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009371MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009371MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009371MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gfi1
  • Uniprot No.：
  • 别名：
    Gfi1; Gfi-1Zinc finger protein Gfi-1; Growth factor independent protein 1
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-423
  • 氨基酸序列
    MPRSFLVKSK KAHSYHQPRS PGPDYSLRLE TVPAPGRAEG GAVSAGESKM EPRERLSPDS QLTEAPDRAS ASPNSCEGSV CDPCSEFEDF WRPPSPSVSP ASEKSLCRSL DEAQPYTLPF KPYAWSGLAG SDLRHLVQSY RQCSALERSA GLSLFCERGS EPGRPAARYG PEQAAGGAGA GQPGRCGVAG GATSAAGLGL YGDFAPAAAG LYERPSTAAG RLYQDHGHEL HADKSVGVKV ESELLCTRLL LGGGSYKCIK CSKVFSTPHG LEVHVRRSHS GTRPFACEMC GKTFGHAVSL EQHKAVHSQE RSFDCKICGK SFKRSSTLST HLLIHSDTRP YPCQYCGKRF HQKSDMKKHT FIHTGEKPHK CQVCGKAFSQ SSNLITHSRK HTGFKPFGCD LCGKGFQRKV DLRRHRETQH GLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.
  • 基因功能参考文献：
    1. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. PMID: 28387757
    2. AMPK was sufficient to stimulate osteogenesis of MC3T3-E1 cells and inhibit adipogenesis of 3T3-L1 cells through the AMPK-Gfi1-OPN axis. PMID: 27283242
    3. If Gfi1 levels fall below a threshold, Id1 expression increases and renders E2A unable to function, which prevents hematopoietic progenitors from engaging along the B lymphoid lineage PMID: 27467586
    4. These results clearly demonstrate that Gfi1 is a critical transcriptional regulator that controls the development of CD4pos and NK1.1pos Invariant natural killer T cells. PMID: 27284976
    5. this study demonstrates the critical role of Gfi1 in the regulation of myeloid cells, and prevention of spontaneous lupus autoimmunity by negatively controlling TLR7 signaling PMID: 27600904
    6. is crucial in the process of macrophage polarization, since its absence impedes macrophage polarization towards a leukemia supporting state and favors an anti-tumor state both in vitro and in vivo PMID: 27390361
    7. these findings demonstrate a novel regulatory role of Gfi1 in the regulation of the Th1-type immune response. PMID: 26749286
    8. GFI1 proteins recruit the chromatin-modifying protein LSD1, a member of the CoREST repressive complex, to epigenetically silence the endothelial program in haemogenic endothelium and allow the emergence of blood cells PMID: 26619147
    9. GFI1 inhibits NLRP3 inflammasome activation and IL-1beta secretion in macrophages PMID: 25447538
    10. Gfi-1 is linked to the erythroid gene regulatory network by repressing Id2 expression. PMID: 25051963
    11. results, supported by evidence from mouse models, identify GFI1 and GFI1B as prominent medulloblastoma oncogenes and implicate 'enhancer hijacking' as an efficient mechanism driving oncogene activation in a childhood cancer PMID: 25043047
    12. Gfi1 and gfi1b repress rag transcription in plasmacytoid dendritic cells in vitro. PMID: 24086657
    13. Our data indicate that Gfi1 is required for hematopoietic precursors to withstand Notch1 activation and to maintain Notch1 dependent transcriptional programming to determine early T-lymphoid lineage identity. PMID: 24068942
    14. Data indicate that Gfi1 regulates the activation of type 2 innate lymphoid cells (ILC2 cells) cells during infection with Nippostrongylus brasiliensis. PMID: 24141388
    15. Gfi1 actively controls thymocyte IL-2 production, thereby shaping thymic generation of Treg cells. PMID: 23918371
    16. Compromised IL-7Ralpha expression in TGF-beta-receptor-deficient T cells was associated with increased expression of the Il7ra transcriptional repressor, Gfi-1. PMID: 23932572
    17. High Gfi1 expression is associated with lymphoblastic leukemia. PMID: 23410974
    18. A hypomorphic mutation in the Gfi1 transcriptional repressor results in a novel form of neutropenia characterized by a split pattern of functional responses. PMID: 22684987
    19. the in vivo regulatory role of Gfi1 was specific for CD8(+), and not CD4(+) T cells or immature thymocytes. These results identify Gfi1 as a specific transcriptional repressor of the Il7r gene in CD8 T lymphocytes in vivo. PMID: 22865857
    20. GFI1 and GFI1B are able to trigger, in the absence of RUNX1, the down-regulation of endothelial markers and the formation of round cells, a morphologic change characteristic of endothelial to hematopoietic transition. PMID: 22668850
    21. Data suggest that Gfi1 may have an important role in prolonged multiple myeloma (MM)-induced osteoblast (OB) suppression and provide a new therapeutic target for MM bone disease. PMID: 22042697
    22. Gfi1's ability to protect against apoptosis is essential for hematopoietic stem cell function. In addition, our fi nding show that Gfi1 prevents the development of myeloproliferative diseases. PMID: 21732494
    23. Findings suggest that p150 may link the DNA-bound Gfi1 repressor complex to histones enabling modifications required for transcriptional silencing. PMID: 21570500
    24. Data show that in in vitro endotoxin tolerant macrophages, the expression of Gfi1 mRNA and protein were up-regulated after high dose LPS stimulation, accompanied with a blunted TNF-alpha and IL-6 secretion. PMID: 20401626
    25. Repression of Id2 expression by Gfi-1 is required for B-cell and myeloid development. PMID: 20453161
    26. Gfi1 expression is associated with the generation of hair cells in the inner ear. PMID: 20533399
    27. Data show that mucous and Paneth but not enteroendocrine lineage cells normally express Gfi1. PMID: 20599897
    28. Gfi1 interacts with p65 and inhibits p65-mediated transcriptional transactivation by interfering with p65 binding to target gene promoter DNA PMID: 20547752
    29. expression outside the lymphoid system in granulocytes and activated macrophages PMID: 11810106
    30. Gfi1 induced by IL-4 regulates Th2 cell proliferation PMID: 12049724
    31. Gfi1 is expressed in the developing nervous system, is required for inner ear hair cell differentiation, and its loss causes programmed cell death PMID: 12441305
    32. Gfi-1 not only promotes differentiation of neutrophils but also antagonizes traits of the alternate monocyte/macrophage program. PMID: 12530980
    33. Gfi1 affects development of early, uncommitted c-Kit+ T cell progenitors and CD4/CD8 lineage decision in the thymus. PMID: 12682108
    34. role for auto- and trans-regulation of Gfi1 by GFI1 and GFI1B in maintaining the normal expression patterns of Gfi1 PMID: 15131254
    35. Gfi1 is regulated through an autoregulatory feedback loop PMID: 15252036
    36. Gfi1 is not expressed in the hair cells of Pou4f3 mutant deaf mice. PMID: 15254021
    37. zinc-finger repressor Gfi-1 (growth factor independent 1) unexpectedly restricts proliferation of hematopoietic stem cells; Gfi-1 is essential to restrict HSC proliferation and to preserve HSC functional integrity PMID: 15457180
    38. Gfi1 as a critical transcription factor that controls DC versus macrophage development and dissociates DC maturation and activation. PMID: 15963786
    39. Data show that Gfi1b can replace Gfi1 functionally in haematopoiesis but not in inner ear hair cell development, demonstrating that Gfi1 and Gfi1b have equivalent and domain-dependent, cell type-specific functions. PMID: 16397623
    40. Gfi-1 down-regulates C/EBPepsilon expression; increased expression of C/EBPepsilon as a consequence of loss of Gfi-1 function may be deleterious to the proliferation and survival of early myeloid cells PMID: 16500901
    41. Gfi1b was found to be up-regulated in early stages of B-cell and in a subset of early T-cell development, where Gfi1 is also present, suggesting that cross-regulation of both loci exists but is cell-type specific PMID: 17095621
    42. Reduced Th2 cell expansion in the absence of Gfi-1 was confirmed by the diminished frequency of IL-4-producing cells when these mice were infected with Schistosoma mansoni PMID: 17116877
    43. GFI-1 represses PU.1 activity and lack of this repression in Gfi-1(-/-) myeloid cells contributes to the observed mixed lineage phenotype. PMID: 17197705
    44. The modest proliferation seen in Gfi1(-/-) NE cells is consistent with the previously proposed role for Gfi1 in controlling neuroendocrine cancer growth. PMID: 17377622
    45. Chromatin regulatory proteins CoREST and LSD1 mediate transcriptional repression by Gfi proteins. PMID: 17707228
    46. Gfi1 functions differently in CD4+ & CD8+ T cells by maintaining different genetic programs in both cell types, & appears to be essential for the CD4 helper T cell immune response but dispensable for the function of cytotoxic CD8+ T cells. PMID: 18034420
    47. a common transcriptional program that underlies both human and murine myelopoiesis, and that is central to the pathogenesis of SCN associated with mutations in GFI1. PMID: 18328744
    48. Gfi1 is a regulator of the innate immune response [review] PMID: 18406379
    49. a new function of Gfi1 in the control of IgG isotype production. PMID: 18991277
    50. Gfi-1 plays a critical role both in enhancing Th2 cell expansion and in repressing induction of Th17 and CD103(+) iTreg cells. PMID: 19188499

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: mmu:14581

    STRING: 10090.ENSMUSP00000135884

    UniGene: Mm.2078