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  4. Recombinant Mouse Zinc finger protein Gfi-1b (Gfi1b)

Recombinant Mouse Zinc finger protein Gfi-1b (Gfi1b)

  • 货号：
    CSB-YP009372MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009372MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009372MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009372MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009372MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Gfi1b
  • Uniprot No.：
  • 别名：
    Gfi1bZinc finger protein Gfi-1b; Growth factor independent protein 1B
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-330
  • 氨基酸序列
    MPRSFLVKSK KAHTYHQPRA QGDELVWPPA VIPVAKEHSQ SASPLLSTPL PSQTLDWNTI KQEREMLLNQ SLPKMASAPE GPLVTPQPQD GESPLSESPP FYKPSFSWDT LASSYSHSYT QTPSTMQSAF LERSVRLYGS PLVPSTESPL DFRLRYSPGM DTYHCVKCNK VFSTPHGLEV HVRRSHSGTR PFACDVCGKT FGHAVSLEQH THVHSQERSF ECRMCGKAFK RSSTLSTHLL IHSDTRPYPC QFCGKRFHQK SDMKKHTYIH TGEKPHKCQV CGKAFSQSSN LITHSRKHTG FKPFSCELCT KGFQRKVDLR RHRESQHNLK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
  • 基因功能参考文献：
    1. GFI1B is an essential protein for the normal development of the megakaryocyte lineage PMID: 27122003
    2. We conclude that Gfi1b controls major functions of megakaryocytes such as integrin-dependent cytoskeleton organization, spreading and migration through the regulation of PAK activity whereas the proplatelet formation defect in GFI1B-deficient megakaryocytes is due, at least partially, to an insufficient alpha-tubulin content. PMID: 28082345
    3. these results identify Rgs18 as a new and crucial effector of Gfi1b that regulates downstream signaling and gene expression programs to orchestrate erythro-megakaryocytic lineage choices. PMID: 26567214
    4. Gfi1b is a regulator of embryonic globin expression and embryonic and adult erythroid maturation. PMID: 24800817
    5. Gfi-1b directly regulates a wide spectrum of megakaryocytic and erythroid genes, predominantly repressing their expression. PMID: 24711581
    6. There is direct yet differential regulation of meis1 transcription by Gfi1b in distinct hematopoietic lineages. PMID: 23308270
    7. Gfi family members are essential for normal B cell development and play an important role in modulating expression of the V(D)J recombinase. PMID: 22201127
    8. The transcription factor Gfi1b defines the brush cell lineage and its progenitors, and with Hes1 and Atoh1 forms a fate-determining Notch signaling driven ternary switch in the developing intestinal epithelium. PMID: 22185794
    9. adult mice conditionally deficient for Gfi1b show a significant expansion of functional HSCs in the bone marrow and blood PMID: 20826720
    10. Gfi1b can auto-repress its own expression, but, in addition, is also able to cross-repress expression of the Gfi1 gene. PMID: 15718298
    11. Results suggest that Gfi1b functions in heterochromatin formation and silencing of euchromatic transcription by recruiting histone methyl transferases to either gamma-satellite sequences or specific target gene promoters. PMID: 16688220

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  • 亚细胞定位：
    Nucleus. Note=Localized at foci of pericentric heterochromatin.
  • 组织特异性：
    Expressed in bone marrow and in spleen. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Expressed in thymocytes.
  • 数据库链接：

    KEGG: mmu:14582

    UniGene: Mm.373385