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  4. Recombinant Mouse Zinc finger protein ZIC 3 (Zic3)

Recombinant Mouse Zinc finger protein ZIC 3 (Zic3)

  • 货号：
    CSB-YP720304MO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP720304MO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP720304MO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP720304MO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP720304MO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Zic3Zinc finger protein ZIC 3; Zinc finger protein of the cerebellum 3
  • 种属：
    Mus musculus (Mouse)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-466
  • 氨基酸序列
    MTMLLDGGPQ FPGLGVGSFG APRHHEMPNR EPAGMGLNPF GDSTHAAAAA AAAAAFKLSP ATAHDLSSGQ SSAFTPQGSG YANALGHHHH HHHHHHASQV PTYGGAASAA FNSTRDFLFR QRGSGLSEAA SGGGQHGLFA GSASSLHAPA GIPEPPSYLL FPGLHEQGAG HPSPTGHVDN NQVHLGLRGE LFGRADPYRP VASPRTDPYA ASAQFPNYSP MNMNMGVNVA AHHGPGAFFR YMRQPIKQEL SCKWIEEAQL SRPKKSCDRT FSTMHELVTH VTMEHVGGPE QNNHVCYWEE CPREGKSFKA KYKLVNHIRV HTGEKPFPCP FPGCGKIFAR SENLKIHKRT HTGEKPFKCE FEGCDRRFAN SSDRKKHMHV HTSDKPYICK VCDKSYTHPS SLRKHMKVHE SQGSDSSPAA SSGYESSTPP AIASANSKDT TKTPSAVQTS TSHNPGLPPN FNEWYV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as transcriptional activator. Required in the earliest stages in both axial midline development and left-right (LR) asymmetry specification. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'.
  • 基因功能参考文献：
    1. When transduced with Oct4, Sox2, and Klf4 (OSK) into murine fibroblasts, Zic3 and Esrrb synergistically enhance the reprogramming efficiency by regulating cellular metabolic pathways. PMID: 28467928
    2. Targeted Resequencing of 29 Candidate Genes and Mouse Expression Studies Implicate ZIC3 and FOXF1 in Human VATER/VACTERL Association PMID: 26294094
    3. The primary effect of Zic3 in heart development occurs during left-right patterning. PMID: 23999067
    4. Zic3 is required in the migrating primitive streak for node morphogenesis and left-right patterning PMID: 23303524
    5. Heterotaxy in patients with premature termination codon-containing ZIC3 transcripts probably arises due to loss of ZIC3 function alone. PMID: 23471918
    6. These data provide basis for further understanding the molecular mechanisms underlying the complex interaction of Zic3 with signaling pathways involved in the early establishment of laterality. PMID: 23184148
    7. study demonstrates that Zic3 and Gli3 expression overlap in developing limbs and that Zic3 converts Gli3 from repressor to activator in vitro; results indicate two Gli superfamily members that cause disparate human congenital malformation syndromes interact genetically and demonstrate the importance of Zic3 in regulating Shh pathway in developing limbs PMID: 22234993
    8. Data show that transcript, termed Zic3-B, encompasses exons 1, 2, and 4 whereas Zic3-A encompasses exons 1, 2, and 3. PMID: 21858219
    9. Data demonstrate an important pathway for regulation of Nanog expression in pluripotent ESCs through direct activation by Zic3. PMID: 20872845
    10. A complex syndrome of left-right axis, central nervous system and axial skeleton defects in Zic3 mutant mice. PMID: 11959836
    11. Zic3-deficient mice develop neural tube defects in their midbrain and hindbrain region and cerebellar dysgenesis characterized by subregional hypoplasia affecting locomotor activity, muscle tone and eye movement control. PMID: 15450095
    12. Deficiency of Zic3 results in abnormal mesoderm allocation. PMID: 16397896
    13. Interacts genetically with Nodal in left-right patterning and subsequent cardiac development. PMID: 16496285
    14. Mice bearing an altered Zic3 allele showed axial skeleton defects, congenital heart defects, and abnormalities of neural crest derivatives, including zygomatic bone, mandible, hyoid bone and laryngeal cartilage. PMID: 17127413
    15. Zic3 plays an important role in the maintenance of pluripotency by preventing endodermal lineage specification in embryonic stem cells. PMID: 17267691
    16. ZIC3 has a role in regulating cardiac gene expression PMID: 17468179

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    GLI C2H2-type zinc-finger protein family
  • 组织特异性：
    CNS. A high level expression is seen in the cerebellum.
  • 数据库链接：

    KEGG: mmu:22773

    STRING: 10090.ENSMUSP00000085999

    UniGene: Mm.255890