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  4. Recombinant Neisseria flavescens Probable peptidoglycan D,D-transpeptidase PenA (penA)

Recombinant Neisseria flavescens Probable peptidoglycan D,D-transpeptidase PenA (penA)

  • 货号：
    CSB-YP323473NFY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP323473NFY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP323473NFY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP323473NFY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP323473NFY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    penA
  • Uniprot No.：
  • 别名：
    penA; Probable peptidoglycan D,D-transpeptidase PenA; EC 3.4.16.4; Penicillin-binding protein 2; PBP-2; Fragment
  • 种属：
    Neisseria flavescens
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-400
  • 氨基酸序列
    NIDGKGQEGL ELSREDSLRG EDGAKVVLRD NKGNIVDSLD SPRNSVPKNG QDMILSLDQR IQTLAYDELN KAVAYHKAKA GAVVVLDAQT GEILALVNSP AYDPNQPGQA NSEQRRNRAV TDMIEPGSAM KPFTIAKALD SGKVDPTDTF NTLPYKIGPA TVQDTHVYPT LDVRGIMQKS SNVGTSKLSA MFTPKEMYDF YHDLGVGVRM HSGFPGETAG LLRSWRRWQK IEQATMSFGY GLQLSLLQLA RAYTVLTHDG ELLPVSFEKQ AVAPKGKRVI KASTAKKVRE LMVSVTEAGG TGIAGAVDGF DVGAKTGTAR KLVNGRYVDN KHVGTFIGFA PAKNPRVIVA VTIDEPTANG YYGGVVAGPV FKEVMSGSLN ILGVSPTKPL SNTATVKVPS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Please can you provide some informations for this protein?
Have you received any customer feedback about the activity of this protein?
Has the tag ever been successfully removed?
I am wondering whether this protein can be produced: 1) without any Tags; 2) with GST-Tag?

A:
Very nice to receive your inquiry.
Expression Region: 1-400aa; Full length.
Tag information: Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

NIDGKGQEGLELSREDSLRGEDGAKVVLRDNKGNIVDSLDSPRNSVPKNGQDMILSLDQRIQTLAYDELNKAVAYHKAKAGAVVVLDAQTGEILALVNSPAYDPNQPGQANSEQRRNRAVTDMIEPGSAMKPFTIAKALDSGKVDPTDTFNTLPYKIGPATVQDTHVYPTLDVRGIMQKSSNVGTSKLSAMFTPKEMYDFYHDLGVGVRMHSGFPGETAGLLRSWRRWQKIEQATMSFGYGLQLSLLQLARAYTVLTHDGELLPVSFEKQAVAPKGKRVIKASTAKKVRELMVSVTEAGGTGIAGAVDGFDVGAKTGTARKLVNGRYVDNKHVGTFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVAGPVFKEVMSGSLNILGVSPTKPLSNTATVKVPS


As this is a semi-custom protein that we haven't expressed before, so we have never tested the activity, we can't find any literature about the activity of this protein, we can't 100% guarantee its activity,
In theory, what we provide is the full length protein and we use affinity chromatography method for purification, it's purified in a very mild environment, it should be active.
Sorry, as this is a semi-custom protein that we haven't expressed before,we also have never tried tag removal.
① Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order.
Sorry, we haven't tried to remove the tag before. We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%.
* Not all protein tags can be removed as some proteins will be very unstable after tag removal.
* If we succeed in removing the tag, we will charge for extra cost.
* If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
* Generally, the delivery time will be extended for 3 days.
②If you need the GST tagged protein, please remark your requirement when placing the order.
We can try to express your desired tag first if you have special request for the tag, but as we can't guarantte, if it's failed, we can provide protein with other tag. The success rate of expressing the protein with your desired tag is 95.25%.

靶点详情

  • 功能：
    Catalyzes cross-linking of the peptidoglycan cell wall at the division septum.
  • 亚细胞定位：
    Cell inner membrane; Single-pass membrane protein.
  • 蛋白家族：
    Transpeptidase family, FtsI subfamily