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  4. Recombinant Phormidium lapideum Glutamine synthetase, partial

Recombinant Phormidium lapideum Glutamine synthetase, partial

  • 货号：
    CSB-YP009553PIC
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP009553PIC
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP009553PIC-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP009553PIC
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP009553PIC
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    N/A
  • Uniprot No.：
  • 别名：
    Glutamine synthetase; GS; EC 6.3.1.2; Glutamate--ammonia ligase; Glutamine synthetase I beta; GSI beta; Fragment
  • 种属：
    Phormidium lapideum
  • 蛋白长度：
    Partial
  • 表达区域：
    1-23
  • 氨基酸序列
    TTPQEVLSRI KDQGIKLIDL KFI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

问答及客户评论

■ 常见问题解答
Q:

Regarding your protein CSB-BP009553PIC, could you please advise on the lead time, the sequencing and the expected tag information?
Also, have you made this protein before, and do you know if it is suitable for lyophilization?
Thanks!

A:
Very nice to receive your inquiry.
Recombinant Phormidium lapideum Glutamine synthetase,partial
CSB-BP009553PIC >> Baculovirus
Expression Region: 1-23aa; Partial.
Tag information: Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 6xHis-tagged.
Sequence:

TTPQEVLSRIKDQGIKLIDLKFI


Reference: https://www.uniprot.org/uniprot/P20479
Note:
At present, this species has not been widely studied, and there is only one region in the Uniprot database, so please confirm whether the sequence listed above is what you want. We haven't expressed this protein before, so the tag type will be determined during the manufacturing process. The expected tag is N-terminal 6xHis-tagged.
Proteins are sensitive to heat and lyophilization maximizes retain protein's activity during storage and prolongs shelf life.
The possible effects of lyophilization on proteins include: protein partial inactivation, aggregation, and other denaturing problems.
However, these negative effects can be minimized by adding protective agents (stabilizers, additives, excipients) and through controlling various conditions of lyophilization.
In general, most of the proteins are suitable for lyophilization. Currently there is no data to indicate that this protein is not suitable for lyophilization.
Therefore, if you need lyophilized form, please remark when placing the order, we can provide the lyophilization service free of charge. Lead time will be extended for 3 working day. We will add a protective agent (6% trehalose) during the lyophilization.

靶点详情

  • 功能：
    Involved in nitrogen metabolism via ammonium assimilation. Catalyzes the ATP-dependent biosynthesis of glutamine from glutamate and ammonia.
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Glutamine synthetase family