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  4. Recombinant Pig Cellular tumor antigen p53 (TP53)

Recombinant Pig Cellular tumor antigen p53 (TP53)

  • 货号：
    CSB-YP880005PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP880005PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP880005PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP880005PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP880005PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    TP53; P53; Cellular tumor antigen p53; Tumor suppressor p53
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-386
  • 氨基酸序列
    MEESQSELGV EPPLSQETFS DLWKLLPENN LLSSELSLAA VNDLLLSPVT NWLDENPDDA SRVPAPPAAT APAPAAPAPA TSWPLSSFVP SQKTYPGSYD FRLGFLHSGT AKSVTCTYSP ALNKLFCQLA KTCPVQLWVS SPPPPGTRVR AMAIYKKSEY MTEVVRRCPH HERSSDYSDG LAPPQHLIRV EGNLRAEYLD DRNTFRHSVV VPYEPPEVGS DCTTIHYNFM CNSSCMGGMN RRPILTIITL EDASGNLLGR NSFEVRVCAC PGRDRRTEEE NFLKKGQSCP EPPPGSTKRA LPTSTSSSPV QKKKPLDGEY FTLQIRGRER FEMFRELNDA LELKDAQTAR ESGENRAHSS HLKSKKGQSP SRHKKPMFKR EGPDSD
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Its pro-apoptotic activity is activated via its interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2. However, this activity is inhibited when the interaction with PPP1R13B/ASPP1 or TP53BP2/ASPP2 is displaced by PPP1R13L/iASPP. In cooperation with mitochondrial PPIF is involved in activating oxidative stress-induced necrosis; the function is largely independent of transcription. Prevents CDK7 kinase activity when associated to CAK complex in response to DNA damage, thus stopping cell cycle progression. Induces the transcription of long intergenic non-coding RNA p21 (lincRNA-p21) and lincRNA-Mkln1. LincRNA-p21 participates in TP53-dependent transcriptional repression leading to apoptosis and seems to have an effect on cell-cycle regulation. Regulates the circadian clock by repressing CLOCK-ARNTL/BMAL1-mediated transcriptional activation of PER2.
  • 基因功能参考文献：
    1. Inhibition of p53 signaling pathway can reverse the cell cycle arrest in G0/G1 phase induced by Porcine epidemic diarrhea virus infection. PMID: 29800601
    2. in a pig model of human familial adenomatous polyposis, p53 plays a role in the early precancerous stages of polyp development PMID: 30145695
    3. Knockdown of p53 led to three outcomes: 1) cell death was attenuated; 2) Tunicamycin-induced redistribution of GRP78 from the nucleus to the endoplasmic reticulum lumen was recovered; and 3) the endoplasmic reticulum-fluorescence/EGFP-calreticulin was recovered. PMID: 30121649
    4. This study demonstrated that various expression levels of p53 in porcine fibroblasts could be achieved by gene targeting and RNA interference. PMID: 27142766
    5. SIRT1, p53 and NF-kappaB are involved in the control of both the proliferation and the apoptosis of ovarian cells. PMID: 25027053
    6. Taken together,these results demonstrated that Porcine parvovirus infection induced apoptosis in PK-15 cells through activation of p53 and mitochondria-mediated apoptosis pathway. PMID: 25499817
    7. Data indicate that higher hepatic glucocorticoid receptor (GR) expression in EHL piglets attributes mainly to the enhanced transcription of GR promoter 1-9/10 from breed-specific interaction of p53 and specificity protein 1 (Sp1). PMID: 23950944
    8. Taken together, these results demonstrated that transmissible gastroenteritis virus infection promoted the activation of p38 MAPK and p53 signalling, and p53 signalling might play a dominant role in the regulation of cell apoptosis. PMID: 23677787
    9. role of transcription factor p53 and the metabolic hormone leptin, in controlling basic functions (proliferation, apoptosis and secretory activity) of ovarian cells PMID: 22151798
    10. differential expression of p53 pathway-related genes accounts for breed-specific skeletal muscle and meat characteristics PMID: 19937136
    11. CONCLUSION(S): (1) Apoptosis is involved in follicular atresia; (2) Bcl-2 is induced by warm ischemia; and (3) cryopreservation insult does not alter the apoptotic signals with short tissue preparation time. PMID: 16616078
    12. Suppressive effect of FSH and LH on p53 expression and caspase-3 activity with parallel increase in bcl-2 expression and increased P production was observed. PMID: 17437340
    13. p53 signaling might be a major determinant for the replicative senescence in the miniature pig fibroblast cells that have the shorter lifespan and slower growth rate compared to primary domestic pig fibroblast cells in vitro. PMID: 17543094
    14. exogenous in vivo NO treatment seems to preserve VSMC from mitochondrial-dependent apoptosis and drive cells to quiescence through p53 increase PMID: 19124124

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  • 相关疾病：
    p53 is found in increased amounts in a wide variety of transformed cells. p53 is frequently mutated or inactivated in many types of cancer.
  • 亚细胞定位：
    Cytoplasm. Nucleus. Nucleus, PML body. Endoplasmic reticulum. Mitochondrion matrix. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
  • 蛋白家族：
    P53 family
  • 数据库链接：

    KEGG: ssc:397276

    STRING: 9823.ENSSSCP00000019016

    UniGene: Ssc.15917