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  4. Recombinant Pig Chymotrypsin-like elastase family member 1 (CELA1)

Recombinant Pig Chymotrypsin-like elastase family member 1 (CELA1)

  • 中文名称：
    Recombinant Pig Chymotrypsin-like elastase family member 1(CELA1),Yeast
  • 货号：
    CSB-YP007586PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Pig Chymotrypsin-like elastase family member 1(CELA1),Yeast
  • 货号：
    CSB-EP007586PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Pig Chymotrypsin-like elastase family member 1(CELA1),Yeast
  • 货号：
    CSB-EP007586PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Pig Chymotrypsin-like elastase family member 1(CELA1),Yeast
  • 货号：
    CSB-BP007586PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Pig Chymotrypsin-like elastase family member 1(CELA1),Yeast
  • 货号：
    CSB-MP007586PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CELA1; ELA1Chymotrypsin-like elastase family member 1; EC 3.4.21.36; Elastase-1
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    27-266
  • 氨基酸序列
    VVGG TEAQRNSWPS QISLQYRSGS SWAHTCGGTL IRQNWVMTAA HCVDRELTFR VVVGEHNLNQ NDGTEQYVGV QKIVVHPYWN TDDVAAGYDI ALLRLAQSVT LNSYVQLGVL PRAGTILANN SPCYITGWGL TRTNGQLAQT LQQAYLPTVD YAICSSSSYW GSTVKNSMVC AGGDGVRSGC QGDSGGPLHC LVNGQYAVHG VTSFVSRLGC NVTRKPTVFT RVSAYISWIN NVIASN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts upon elastin.
  • 基因功能参考文献：
    1. insights into the PPE and HNE inhibition PMID: 26372354
    2. Here, we studied the priming of SARS-CoV S by elastase and show an important role for residue Thr(795) in the S2 domain. PMID: 20507992
    3. comparison of x-ray structures of alpha1PI complexes with trypsin, PPE, and human neutrophil elastase PMID: 16321984
    4. Results describe mapping of the binding surface of porcine pancreatic elastase. PMID: 16488429
    5. crystal structure of PPE complexed with a novel trimacrocyclic peptide inhibitor, FR901451, was solved at 1.9 A resolution PMID: 16511165
    6. The crystal structure of the acyl complex of porcine pancreatic elastase with its peptidyl ester substrate N-acetyl-ala-ala-ala-methyl ester (Ac(Ala)3OMe) has been determined at 2.5 A resolution PMID: 17083959
    7. Porcine pancreatic elastase (PPE) was crystallized in complex with a novel inhibitor at pH 5 and X-ray diffraction data were collected at a synchrotron source to 1.66 A. PMID: 17266656
    8. the oxygen of the oxopropyl group of the inhibitor is present as an oxygen anion rather than a hydroxyl group, supporting the role of the "oxyanion hole" in stabilizing the tetrahedral intermediate in ELS1 catalysis PMID: 19603802

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Peptidase S1 family, Elastase subfamily
  • 组织特异性：
    Pancreas. Basal layers of the epidermis, hair follicle and sebaceous gland epithelia (at protein level).
  • 数据库链接：

    KEGG: ssc:396766

    STRING: 9823.ENSSSCP00000026330

    UniGene: Ssc.5575