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  4. Recombinant Pig Clusterin (CLU)

Recombinant Pig Clusterin (CLU)

  • 货号：
    CSB-YP005595PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005595PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005595PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005595PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005595PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CLU; Clusterin; CP40; Complement cytolysis inhibitor; CLI) [Cleaved into: Clusterin beta chain; Clusterin alpha chain]
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    29-446
  • 氨基酸序列
    KE LQEMSTEGSK YVNKEIKNAL KEVKQIKTLI EQSNEERKSL LSSLEEAKKK KEDALNDTRD TETKLKGSQG LCNETMMALW EECKPCLKQT CMKFYARVCR SGSGLVGHQL EEFLNQSSPF YFWINGDRID SLMENDRQQS HVMDIMEDSF NRASNIMDEL FQDRFFNREP FDTQFFSPFG SSHRGSLFFN PKSRFARNIM PFPLFTDLNY HDMFQPFFDM IHQAQQAMDA HLHRIPYHFP EAGVPENSND RAVCKEIRHN STGCLRMKDQ CEKCREILSV DCSASNSSQM QLRQELYTSL QMAEKFSKLY DQLLQSYQQK MLNTSSLLKQ LNEQFSWVSQ LANLTQNDDR YYLQVTTVNS HGSDPSVPSG LTKVVVKLFD SYPITLIIPQ EVSDPKFMET VAEEALQQYR QRSREE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Following stress, promotes apoptosis. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury.
  • 基因功能参考文献：
    1. Data demonstrate that the resistance of neonatal porcine Sertoli cells to human xenoantibody and complement-mediated lysis is associated with low expression of xenoantigen alpha-Gal and high production of the complement inhibitors clusterin and CD59. PMID: 20636542
    2. These results show that clusterin expression in porcine species is found in an own, as yet unidentified cell type during postnatal developmental stages. PMID: 17960512
    3. Porcine spermatozoa may delivery CLU, PRM1 and PRM2 mRNAs to the oocyte, which may contribute to zygotic and early embryonic development. PMID: 18462614
  • 亚细胞定位：
    Secreted. Nucleus. Cytoplasm. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Mitochondrion. Mitochondrion membrane. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, chromaffin granule.
  • 蛋白家族：
    Clusterin family
  • 组织特异性：
    Highest levels in brain and liver; lower levels are detected in other tissues, including the aorta. Abundant in senescent porcine pituitary colloids.
  • 数据库链接：

    KEGG: ssc:397025

    STRING: 9823.ENSSSCP00000010325

    UniGene: Ssc.11992