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  4. Recombinant Pig Endothelin-1 (EDN1)

Recombinant Pig Endothelin-1 (EDN1)

  • 货号：
    CSB-YP007400PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007400PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007400PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007400PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007400PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    EDN1; Endothelin-1; ET-1; Preproendothelin-1; PPET1) [Cleaved into: Big endothelin-1]
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    53-82
  • 氨基酸序列
    CSCSSLMD KECVYFCHLD IIWVNTPEHI VP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
  • 基因功能参考文献：
    1. In conclusion, endothelial vWF knockdown prevented angiotensin II-induced ET-1 upregulation through attenuation of NOX-mediated O2- production. PMID: 27443965
    2. sub-vasomotor concentration of ET-1 leads to vascular dysfunction by impairing endothelium-dependent NO-mediated dilation via p38 kinase-mediated production of superoxide from NADPH oxidase following ETA receptor activation PMID: 26211713
    3. ET1 was lowest in kidneys removed from live pigs, greater in kidneys from pigs with brain death, and greatest in kidneys from pigs with cardiac arrest. PMID: 25380860
    4. ET-1 contributes to formation of oedema during experimental sepsis by a novel mechanism involving increased HBP release from neutrophils. PMID: 24611481
    5. Endothelin-1 expression is upregulated following therapeutic hypothermia after cardiac arrest. PMID: 23717659
    6. Our experiment with isolated porcine ophthalmic ciliary arteries revealed a refractoriness phase to ET after an acute stimulation with ET. This refractoriness was transient and disappeared after 4 h. PMID: 23327561
    7. ET-1 produces contraction of the urinary bladder neck muscles via muscular ET(A) receptors coupled to extracellular Ca(2+) entry via VOC (L-type) and non-VOC channels. PMID: 21953705
    8. urinary biomarker to determine kidney graft injury PMID: 22221604
    9. Endothelin-1 elevation is not only a conserved phenomenon in a pig traumatic brain injury (TBI) model, but it is a likely target for understanding the observed enhanced vascular response to TBI. PMID: 21801588
    10. interleukin-6, endothelin ET-1, and apoptotic Bak and Bcl-XL genes have roles in small bowel transplantation, in a swine model of ischemia and reperfusion injury PMID: 21884347
    11. ET-1 elicits a different pattern of Src family kinase (SFK) activation and might trigger a different pattern of SFK activation from that caused by ATP and UTP. PMID: 21792912
    12. Compared with the untreated group, the levels of serum ET-1 after acute myocardial infarction and reperfusion were significantly decreased in the Xuefu Zhuyu-treated group. PMID: 18405606
    13. study suggests a possible role for endothelin-1(resulting from the actions of endothelin-converting enzyme-1) acting via endothelin receptor A in the control of luteolytic sensitivity in the pig PMID: 19783117
    14. vasoconstrictor influence of endogenous as well as exogenous ET on coronary circulation in vivo is reduced in myocardial infarct PMID: 15458948
    15. Data show the presence of a continuative and differentially regulated stimulating mechanism of ET-1 expression during pig endotoxaemia as well as a fundamental role of ET-1 system in the cardiovascular and respiratory derangement. PMID: 16043243
    16. These data support a linkage between the proinflammatory cytokine IL-1beta and vascular dysfunction within the intestinal circulation, mediated, at least in part, by the ET system. PMID: 16782700
    17. extracellular ET-1 regulates the abundance of ET-1 mRNA in PAECs, in an ETB receptor-dependent manner, by modulating both mRNA stability and transcription via mechanisms involving receptor endocytosis and both ERK and p38 MAPK pathways PMID: 18278064
    18. Cyclosporine-A stimulates angiotensin I/II secretion by kidney glomeruli. PMID: 18331442
    19. Left ventricular unloading with intra-aortic counter pulsation prior to reperfusion reduces myocardial release of endothelin-1 and decreases infarction size in a porcine ischemia-reperfusion model. PMID: 18814226
    20. The beneficial effect of carvedilol on myocardial no-reflow could be due to its effect on ET-1 via the activation of the K(ATP) channel. PMID: 18840580
    21. is not predictive of ventricular fibrillation during acute myocardial ischemia PMID: 18844577
    22. Porcine ET-1 rapidly activates the prosurvival phosphatidylinositol 3'-OH kinase (PI3K)/AKT signaling pathway in normal and fibrotic human lung fibroblasts. PMID: 19188658
    23. Report novel pathway involving endothelin-1 and reactive oxygen species in coronary vasospasm with endothelial dysfunction. PMID: 19623039

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Endothelin/sarafotoxin family
  • 数据库链接：

    KEGG: ssc:396915

    STRING: 9823.ENSSSCP00000001121

    UniGene: Ssc.9364