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  4. Recombinant Pig Insulin-like growth factor II (IGF2)

Recombinant Pig Insulin-like growth factor II (IGF2)

  • 中文名称：
    斯克罗法IGF2重组蛋白
  • 货号：
    CSB-YP011088PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    斯克罗法IGF2重组蛋白
  • 货号：
    CSB-EP011088PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    斯克罗法IGF2重组蛋白
  • 货号：
    CSB-EP011088PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    斯克罗法IGF2重组蛋白
  • 货号：
    CSB-BP011088PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    斯克罗法IGF2重组蛋白
  • 货号：
    CSB-MP011088PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    IGF2Insulin-like growth factor II; IGF-II) [Cleaved into: Insulin-like growth factor II; Preptin]
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    25-91
  • 氨基酸序列
    AYRPSE TLCGGELVDT LQFVCGDRGF YFSRPASRVN RRSRGIVEEC CFRSCDLALL ETYCATPAKS E
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The insulin-like growth factors possess growth-promoting activity. Major fetal growth hormone in mammals. Plays a key role in regulating fetoplacental development. IGF2 is influenced by placental lactogen. Also involved in tissue differentiation. In adults, involved in glucose metabolism in adipose tissue, skeletal muscle and liver. Acts as a ligand for integrin which is required for IGF2 signaling. Positively regulates myogenic transcription factor MYOD1 function by facilitating the recruitment of transcriptional coactivators, thereby controlling muscle terminal differentiation. Inhibits myoblast differentiation and modulates metabolism via increasing the mitochondrial respiration rate.; Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 基因功能参考文献：
    1. These results indicated that editing IGF2 intron 3-3072 site using CRISPR-Cas9 technology improved meat production in Bama pigs. PMID: 30259067
    2. trans-associations occur between three imprinted genes IGF2, DLK1 and MEG3 both in fetal liver and muscle cells. PMID: 27716032
    3. A single nucleotide polymorphism in a regulatory region of intron 3 within the porcine IGF2 gene is associated with increased lean deposition and decreased fat deposition in pigs with paternal A alleles compared with pigs with paternal G alleles. PMID: 25367517
    4. These findings indicated that the mRNA of H19 and IGF2 genes is susceptible to in vitro environments during the process of ES cell derivation from blastocysts but DNA methylation status at this region was well maintained. PMID: 24794444
    5. study did not find any significant associations for polymorphisms in insulin-like grwoth factor 2, GTP Binding Protein alpha Subunits, Gs and melanocortin receptor 4 genes with reproductive traits of Polish Landrace and Large White pigs PMID: 23199214
    6. The results suggested that IFG-1 and -2 and their receptors are differentially expressed at the maternal and fetal components of the attachment site. PMID: 21685713
    7. Genetic variation in the promoter region of the IGF2 gene is associated with intramuscular fat content in porcine skeletal muscle. PMID: 21779802
    8. The IGF2 G allele has strong adipogenic effects at the subcutaneous adipose tissue level and enhances the intermuscular fat content in ham. PMID: 21907500
    9. IGF-I, IGF-II, and IGFBP-3 mRNA were positively correlated with IGF-IR from 50E to 180D, suggesting that the expression of IGF-system genes exhibits specific developmental patterns in the skeletal muscle tissues. PMID: 21570979
    10. The imprinting status in adult liver, muscle and kidney tissues were also not reflected in the methylation patterns of IGF2 DMRs 1 and 2. PMID: 21575277
    11. The expression of IGF-I and IGF-II in native growth plates of prepubertal piglets and under different cell culture conditions, was compared. PMID: 20600587
    12. The aim of this work was to study the effects on litter size of variants of the porcine genes RBP4, ESR1 and IGF2, currently used in genetic tests for different purposes. PMID: 20576168
    13. Promoter-specific expression differences of IGF2 isoforms and IGF2AS between biparental and parthenogenetic swine fetal tissues were documented. IGF2 and IGF2AS are paternally expressed. PMID: 19571260
    14. In both skeletal muscle and heart muscle growth, the insulin-like growth factor-2:myostatin interaction seems to play an important role. PMID: 19674865
    15. Effect of genotype on proteolytic enzyme in skeletal muscles during growth. PMID: 15560245
    16. Mutation controls the paternally expressed QTL for backfat thickness in a cross between Meishan and European Whites. PMID: 15678747
    17. A significant effect of linkage disequilibrium between IGF2-in3-G3072A and IGF2-in7-G162C on backfat thickness and lean meat content was found. PMID: 16706926
    18. Results showed that in IGF2 gene, the predominant allele was C, and the predominant genotype was CC. and that Wuzhishan pig breed was at Hardy-Weinberg equilibrium with respect to this SNP. PMID: 17284425
    19. The majority of IGF2 transcripts are expressed from promoters 2-4 and are imprinted. PMID: 18228173
    20. IGF2 gene has significant effects on individual birth weight and backfat thickness PMID: 18244923
    21. Haplotype variability around a known causative mutation in porcine gene IGF2, which increases lean content, is analysed. PMID: 18245828
    22. The results suggest that the expression of IGF2 and IGFBP3 mRNA in the adipose tissue of pigs exhibits specific developmental changes and different patterns between the two pig breeds. PMID: 18487150
    23. The current findings suggest that IGF-I and IGF-II have different effects on porcine adipose cell development. PMID: 18640238
    24. Methylation status of differentially methylated regions of Igf2/H19 locus in porcine gametes and preimplantation embryos are reported. PMID: 18983907
    25. The selection based on the IGF2 mutation in Poland may be very useful in Polish Landrace and Large White pigs, where the G allele is still relatively frequent. PMID: 19638682

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Insulin family
  • 数据库链接：

    KEGG: ssc:396916

    UniGene: Ssc.9365