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  4. Recombinant Pig Interferon regulatory factor 3 (IRF3)

Recombinant Pig Interferon regulatory factor 3 (IRF3)

  • 货号：
    CSB-YP011818PI
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP011818PI
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP011818PI-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP011818PI
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP011818PI
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    IRF3Interferon regulatory factor 3; IRF-3
  • 种属：
    Sus scrofa (Pig)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-419
  • 氨基酸序列
    MGTQKPRILP WLISQLNQGQ LEGVAWLDEG HTRFRIPWKH GLRQDAQQED FGIFQAWAEA SGAYTPGKDK PDLPTWKRNF RSALNRKEAL RLAEDHSKDP HDPHKIYEFV TSGVGDFPEP DTSLDLSGRY STSDTHEDSL DKLLSGMDLA SDAGPQSLTL ALEQPPQLSL SPSVDAPASC PNLGVRENPL KQLLANDDEW EFQVTVFYRG CQVFQQTVCS PGGLRLVGSE AEDGTLAGQP VRLPDPAASL TDRGVADYVR RVLSCLGGGL ALWRAGQWLW AQRLGHCHVY WAMGEELIPD SGHKPDGEVP KDREGGVFDL GPFIEDLIAF IEGSRRSPRY TLWFCMGQSW PQDEPWVKRL VMVKVVPMCL RALVDMARDG GASSLENTVD LHISNSHPLS LTSDQYKACL RDLVEDMDF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key transcriptional regulator of type I interferon (IFN)-dependent immune responses which plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Acts as a more potent activator of the IFN-beta (IFNB) gene than the IFN-alpha (IFNA) gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. Found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Can activate distinct gene expression programs in macrophages and can induce significant apoptosis in primary macrophages.
  • 基因功能参考文献：
    1. The s used recombinant classical swine fever virus N(pro) and swine IRF3 proteins and show that N(pro) interacts with IRF3 directly without additional proteins and forms a soluble 1:1 complex. PMID: 27334592
    2. Amino acid residues in the N-terminal domain of Npro are involved in the stability of Npro, in interaction of Npro with IRF-3 and subsequent degradation of IRF-3, leading to downregulation of IFN-alpha/beta production. PMID: 25809915
    3. The obtained results showed that PRRSV nsp1 could inhibit Poly(I:C)-induced IFN-beta promoter activity in MARC-145 cells by down-regulating the protein level of IRF-3 and inhibiting the phosphorylation of IRF-3. PMID: 20692306
    4. proteasomal degradation of IRF3 is induced by a direct or indirect interaction with N(pro). PMID: 17215286
  • 亚细胞定位：
    Cytoplasm. Nucleus. Mitochondrion.
  • 蛋白家族：
    IRF family
  • 数据库链接：

    KEGG: ssc:396656

    STRING: 9823.ENSSSCP00000028920

    UniGene: Ssc.79511