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  4. Recombinant Protein pop-1 (pop-1)

Recombinant Protein pop-1 (pop-1)

  • 货号：
    CSB-YP606015CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP606015CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP606015CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP606015CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP606015CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pop-1
  • Uniprot No.：
  • 别名：
    pop-1; W10C8.2; Protein pop-1; Posterior pharynx defect protein 1
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-438
  • 氨基酸序列
    MMADEELGDE VKVFRRDEDA DDDPMISGET SEQQLADDKK EAVMEAELDG AGRNPSIDVL KSAFPKVEPM SPSFPGLMSH FSPGYSAAAL PMFMPLFMNP YAAALRSPSL MFPMGAMSPT FPMFPPSPVY GAAIAAAAAK QHFENMAPLN MRAGHPMNQM GMPPYMHPSS MAPQNVDRRA QGGGKAKKDD HVKKPLNAFM WFMKENRKAL LEEIGNNEKQ SAELNKELGK RWHDLSKEEQ AKYFEMAKKD KETHKERYPE WSARENYAVN KKKTKKRRDK SIPSENNDQK KCRARFGVNN TEMWCKFCKR KKKCEYATDR SGGSDITDSQ DGRGTSGAYS SSSESPSPKA NAGIALTTQQ QQAAMMHTML MQMRLGSTTG ASTHVPSPLA SSSAGRSPLD ANASDSESDV EEEEDEQIDP TVMQQTHDML MQESMCTI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Part of the Wnt signaling pathway essential for the specification of the mesodermal cell fate in early embryos. Required for asymmetrical division of somatic gonadal precursor descendants which initiate axis formation required to control organ shape. Similarly, involved in asymmetrical division of seam cells, a stem cell-like lineage. Represses expression of target genes via its interaction with hda-1 histone deacetylase. Required for specification of the M lineage-derived coelomocyte and sex myoblast fate. Regulates coelomocyte fate by positively regulating proliferation and ceh-34 and possibly eya-1 expression in M.dlpa and M.drpa precursors.
  • 基因功能参考文献：
    1. these results indicate that SYS-1 and POP-1 levels are influenced by the parent cell's SYS-1/POP-1 levels and this may provide an additional mechanism by which POP-1 regulates distinct targets in different developmental contexts. PMID: 26488501
    2. we demonstrate that POP-1 (the C. elegans TCF) also activates target genes through HMG and Helper site interactions. PMID: 24516405
    3. SYS-1 and POP-1 nuclear levels are regulated in opposite directions; the C terminus of POP-1 is essential for LIT-1 phosphorylation and is specifically bound by the diverged beta-catenin WRM-1 PMID: 21852394
    4. Animals with reduced function of the Wnt-pathway downstream effector POP-1 have as many as 67 seam cells. The 'Wnt/beta-catenin asymmetry' pathway is required for the proper stem cell-like asymmetric division of seam cells. PMID: 20849842
    5. Study suggests that pop-1 is not critical for mesoderm specification beyond repression of endoderm specification, and reveals that Wnt-modified pop-1 further reinforces endoderm specification by repressing mesoderm fate in endoderm. PMID: 19818340
    6. acetylation enhances nuclear retention of POP-1, the Caenorhabditis elegans LEF/TCF homolog, through increasing nuclear import and blocking nuclear export PMID: 12651889
    7. Wnt/MAPK signaling downregulates POP-1 levels in responsive cells, in part by increasing nuclear LIT-1 levels, thereby increasing POP-1 phosphorylation and PAR-5-mediated nuclear export. PMID: 15066285
    8. The Wnt effector POP-1 and the PAL-1/Caudal homeoprotein collaborate with SKN-1 to activate C. elegans endoderm development. PMID: 16084508
    9. TCF protein, POP-1, converts from repressor to activator as a result of Wnt-induced lowering of nuclear levels. PMID: 16112103
    10. POP-1/TCF and SYS-1/beta-catenin directly activate transcription of ceh-22/nkx2.5 isoforms in somatic gonadal precursor distal daughter cells. PMID: 16461282
    11. The Wnt effector POP-1, its coactivator SYS-1/beta-catenin, and of upstream components including beta-catenin WRM-1 causes all SGP daughters to adopt the proximal fate. PMID: 16890160
    12. Reciprocal asymmetry of SYS-1/beta-catenin and POP-1/TCF controls asymmetric divisions in Caenorhabditis elegans. PMID: 17296929
    13. There are two genetic pathways, one increasing SYS-1 and the other decreasing POP-1 levels, robustly elevate the SYS-1-to-POP-1 ratio in the posterior cell, thereby driving A-P differential cell fates. PMID: 17567664
    14. Integration of Wnt-dependent and Wnt-independent cell fate specification pathways within the Caenorhabditis genus can occur in different ways. PMID: 18977344

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 蛋白家族：
    TCF/LEF family
  • 数据库链接：

    KEGG: cel:CELE_W10C8.2

    STRING: 6239.W10C8.2

    UniGene: Cel.19670