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  4. Recombinant Pseudomonas aeruginosa 2-aminobenzoylacetyl-CoA thioesterase (pqsE)

Recombinant Pseudomonas aeruginosa 2-aminobenzoylacetyl-CoA thioesterase (pqsE)

  • 货号：
    CSB-YP322665EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP322665EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP322665EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP322665EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP322665EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pqsE
  • Uniprot No.：
  • 别名：
    pqsE; PA10002-aminobenzoylacetyl-CoA thioesterase; EC 3.1.2.32
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-301
  • 氨基酸序列
    MLRLSAPGQL DDDLCLLGDV QVPVFLLRLG EASWALVEGG ISRDAELVWA DLCRWVADPS QVHYWLITHK HYDHCGLLPY LCPRLPNVQV LASERTCQAW KSESAVRVVE RLNRQLLRAE QRLPEACAWD ALPVRAVADG EWLELGPRHR LQVIEAHGHS DDHVVFYDVR RRRLFCGDAL GEFDEAEGVW RPLVFDDMEA YLESLERLQR LPTLLQLIPG HGGLLRGRLA ADGAESAYTE CLRLCRRLLW RQSMGESLDE LSEELHRAWG GQSVDFLPGE LHLGSMRRML EILSRQALPL D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the biosynthesis of the quorum-sensing signaling molecules 2-heptyl-4(1H)-quinolone (HHQ) and 2-heptyl-3-hydroxy-4(1H)-quinolone (Pseudomonas quinolone signal or PQS), which are important for biofilm formation and virulence. Catalyzes the hydrolysis of the intermediate 2-aminobenzoylacetyl-CoA (2-ABA-CoA) to form 2-aminobenzoylacetate (2-ABA), the precursor of HHQ. In vitro, can also hydrolyze other substrates such as S-ethyl-acetothioacetate and acetoacetyl-CoA, but is inactive against anthraniloyl-CoA, malonyl-CoA and octanoyl-CoA. Beyond its thioesterase function, is involved in the regulation of diverse genes coding for key virulence determinants and biofilm development.
  • 基因功能参考文献：
    1. Results indicate the importance of a unique C-terminal alpha-helical motif in the molecular function of PqsE. PMID: 24040042
    2. Data suggest that PqsR exhibits a ligand binding pocket for endogenous and synthetic quinolone regulators; PqsR and these ligands are likely regulators of quorum sensing. PMID: 23935486
    3. Data reveal that PqsE is a key regulator within the quorum sensing circuitry facilitating the environmental adaptation of Pseudomonas aeruginosa. PMID: 20406282
    4. Data show that PqsE can activate PQS-controlled genes in the absence of PqsR and PQS, and that the regulatory activity of PqsE requires RhlR and indicate that a pqsE mutant can be complemented for pyocyanin production by a large excess of C4-HSL. PMID: 18776012
  • 蛋白家族：
    Metallo-beta-lactamase superfamily
  • 数据库链接：

    KEGG: pae:PA1000

    STRING: 208964.PA1000