Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Pseudomonas aeruginosa Cyclic AMP receptor-like protein (vfr)

Recombinant Pseudomonas aeruginosa Cyclic AMP receptor-like protein (vfr)

  • 中文名称：
    铜绿假单胞菌vfr重组蛋白
  • 货号：
    CSB-YP346227EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    铜绿假单胞菌vfr重组蛋白
  • 货号：
    CSB-EP346227EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    铜绿假单胞菌vfr重组蛋白
  • 货号：
    CSB-EP346227EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    铜绿假单胞菌vfr重组蛋白
  • 货号：
    CSB-BP346227EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    铜绿假单胞菌vfr重组蛋白
  • 货号：
    CSB-MP346227EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    vfr
  • Uniprot No.：
  • 别名：
    vfr; PA0652; Cyclic AMP receptor-like protein
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-214
  • 氨基酸序列
    MVAITHTPKL KHLDKLLAHC HRRRYTAKST IIYAGDRCET LFFIIKGSVT ILIEDDDGRE MIIGYLNSGD FFGELGLFEK EGSEQERSAW VRAKVECEVA EISYAKFREL SQQDSEILYT LGSQMADRLR KTTRKVGDLA FLDVTGRVAR TLLDLCQQPD AMTHPDGMQI KITRQEIGRI VGCSREMVGR VLKSLEEQGL VHVKGKTMVV FGTR
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Global cAMP-dependent transcriptional regulator that controls virulence gene expression by distinct cAMP-dependent and -independent mechanisms, which allow to fine tune its virulence program in response to specific host cues or environments. Controls the expression of many regulatory targets including type II, type III and type IV secretion systems, flagellar-mediated motility, and quorum sensing systems. Transcriptional control is exerted by binding to a well-characterized consensus site (5'-ANWWTGNGAWNYAGWTCACAT) within target promoters. Directly binds to the toxA upstream region to regulate exotoxin A production, to the lasR gene promoter to activate the las quorum-sensing system or to the exsA promoter to regulate type III secretion system. Autoregulates as well its own expression.
  • 基因功能参考文献：
    1. Vfr directly regulates rhlR transcription through its binding to several Vfr-binding sites (VBSs) present in the rhlR promoter region. PMID: 21719541
    2. The crystal structure of Vfr shows that it is a winged-helix DNA-binding protein like its homologue cyclic AMP receptor protein (CRP). PMID: 21665969
    3. study suggests that Vfr shares many of the same functions as cAMP receptor protein, CRP, but has specialized functions, at least in terms of DNA target sequence binding, required for regulation of a subset of genes in its regulon PMID: 17159200
    4. In a reconstruction experiment conducted with a 1:1 mixture of wild-type and a vfr mutant of PAO1, data showed that the vfr mutant had a selective advantage over the wild type after growth in static culture for 4 days. PMID: 18203852
    5. The results suggest that Vfr modulates toxA and lasB expression in PAO1 through PtxR; a model defining the relationships between these different genes is presented. PMID: 18227247
    6. Vfr enhances toxA expression in PAO1 both directly and indirectly through regA, but not through pvdS;vfr expression is not regulated by iron;both Vfr and PvdS cooperate in the presence of RegA to achieve a maximum level of toxA expression. PMID: 19389782

    显示更多

    收起更多

  • 数据库链接：

    KEGG: pae:PA0652

    STRING: 208964.PA0652