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  4. Recombinant Pseudomonas aeruginosa L-ornithine 5-monooxygenase (pvdA)

Recombinant Pseudomonas aeruginosa L-ornithine 5-monooxygenase (pvdA)

  • 货号：
    CSB-YP706553EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP706553EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP706553EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP706553EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    pvdA
  • Uniprot No.：
  • 别名：
    pvdA; pvd-1; PA2386; L-ornithine N(5)-monooxygenase; EC 1.14.13.195; L-ornithine N(5)-hydroxylase; Ornithine hydroxylase; L-ornithine N(5)-oxygenase; Pyoverdin biosynthesis protein A
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-443
  • 氨基酸序列
    MTQATATAVV HDLIGVGFGP SNIALAIALQ ERAQAQGALE VLFLDKQGDY RWHGNTLVSQ SELQISFLKD LVSLRNPTSP YSFVNYLHKH DRLVDFINLG TFYPCRMEFN DYLRWVASHF QEQSRYGEEV LRIEPMLSAG QVEALRVISR NADGEELVRT TRALVVSPGG TPRIPQVFRA LKGDGRVFHH SQYLEHMAKQ PCSSGKPMKI AIIGGGQSAA EAFIDLNDSY PSVQADMILR ASALKPADDS PFVNEVFAPK FTDLIYSREH AERERLLREY HNTNYSVVDT DLIERIYGVF YRQKVSGIPR HAFRCMTTVE RATATAQGIE LALRDAGSGE LSVETYDAVI LATGYERQLH RQLLEPLAEY LGDHEIGRDY RLQTDERCKV AIYAQGFSQA SHGLSDTLLS VLPVRAEEIS GSLYQHLKPG TAARALHEHA LAS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the conversion of L-ornithine to N(5)-hydroxyornithine, the first step in the biosynthesis of all hydroxamate-containing siderophores, such as pyoverdin. Pyoverdin is a hydroxamate siderophore composed of a 6,7-dihydroxyquinoline-containing fluorescent chromophore joined to the N-terminus of a partly cyclic octapeptide (D-Ser-L-Arg-D-Ser-L-N(5)-OH-Orn-L-Lys-L-N(5)-OH-Orn-L-Thr-L-Thr in strain PAO1). Specific for NADPH, which plays a role in stabilization of the C4a-hydroperoxyflavin intermediate.
  • 基因功能参考文献：
    1. PvdA is a membrane-associated protein located at the old cell pole PMID: 22498339
    2. The first two structures of a class B N-hydroxylating monooxygenase were determined with FAD in oxidized (1.9 A resolution) and reduced (3.03 A resolution) states PMID: 21757711
    3. overexpression and purification of active recombinant L-ornithine N5-hydroxylase, PvdA; detailed steady-state kinetic analysis to determine the substrate and coenzyme specificities PMID: 17015659
    4. The membrane topogenic determinants of PvdA were studies, and PvdA appears to have a single N-terminal domain anchored to the inner membrane. PMID: 18757814
    5. PvdA employs a kinetic mechanism that is a hybrid of those previously documented for other flavin-dependent hydroxylases PMID: 19368334

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  • 亚细胞定位：
    Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
  • 蛋白家族：
    Lysine N(6)-hydroxylase/L-ornithine N(5)-oxygenase family
  • 数据库链接：

    KEGG: pae:PA2386

    STRING: 208964.PA2386