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  4. Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein (algR)

Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein (algR)

  • 中文名称：
    Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein(algR),Yeast
  • 货号：
    CSB-YP335184EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein(algR),Yeast
  • 货号：
    CSB-EP335184EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein(algR),Yeast
  • 货号：
    CSB-EP335184EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein(algR),Yeast
  • 货号：
    CSB-BP335184EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Pseudomonas aeruginosa Positive alginate biosynthesis regulatory protein(algR),Yeast
  • 货号：
    CSB-MP335184EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    algR
  • Uniprot No.：
  • 别名：
    algR; PA5261Positive alginate biosynthesis regulatory protein
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-248
  • 氨基酸序列
    MNVLIVDDEP LARERLARLV GQLDGYRVLE PSASNGEEAL TLIDSLKPDI VLLDIRMPGL DGLQVAARLC EREAPPAVIF CTAHDEFALE AFQVSAVGYL VKPVRSEDLA EALKKASRPN RVQLAALTKP PASGGSGPRS HISARTRKGI ELIPLEEVIF FIADHKYVTL RHAQGEVLLD EPLKALEDEF GERFVRIHRN ALVARERIER LQRTPLGHFQ LYLKGLDGDA LTVSRRHVAG VRRLMHQL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positive regulator of the algD gene, which codes for a GDP-mannose dehydrogenase, a key step enzyme in the alginate biosynthesis pathway.
  • 基因功能参考文献：
    1. In conclusion, this study for the first time indicates that AlgR directly binds to rsmZ to regulates the expression of lipA via regulating transcription of rsmZ, and mainly regulates the expression of lipA at transcriptional level in P. protegens Pf-5. PMID: 28400281
    2. AlgR controls the levels of c-di-GMP synthesis via direct regulation of mucR, which encodes a diguanylate cyclase. PMID: 26206672
    3. AlgZ and algR transcription involves multiple factors at multiple start sites that control individual gene expression. PMID: 25488298
    4. These results indicate that phosphorylated AlgR-P has increased affinity for the rhlA promoter and is required for the coordinate activation of twitching motility, rhamnolipid production, and swarming motility in P. aeruginosa. PMID: 24097945
    5. AlgR specifically represses the Rhl quorum-sensing system during biofilm growth. PMID: 17766417
    6. Findings indicate that AlgR is essential for proper pilin localization and that phosphorylation of AlgR results in direct activation of the fimU-pilVWXY1Y2E operon, which is required for the assembly and export of a functional type IV pilus. PMID: 18178737
    7. Strains lacking AlgR or the putative AlgR sensor AlgZ produced significantly less hydrogen cyanide than did a nonmucoid isogenic parent. PMID: 19270096

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  • 数据库链接：

    KEGG: pae:PA5261

    STRING: 208964.PA5261