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  4. Recombinant Pseudomonas aeruginosa RNA polymerase sigma-54 factor (rpoN)

Recombinant Pseudomonas aeruginosa RNA polymerase sigma-54 factor (rpoN)

  • 中文名称：
    铜绿假单胞菌rpoN重组蛋白
  • 货号：
    CSB-YP343451EZX
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoN重组蛋白
  • 货号：
    CSB-EP343451EZX
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoN重组蛋白
  • 货号：
    CSB-EP343451EZX-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoN重组蛋白
  • 货号：
    CSB-BP343451EZX
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    铜绿假单胞菌rpoN重组蛋白
  • 货号：
    CSB-MP343451EZX
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rpoN
  • Uniprot No.：
  • 别名：
    rpoN; PA4462; RNA polymerase sigma-54 factor
  • 种属：
    Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-497
  • 氨基酸序列
    MKPSLVLKMG QQLTMTPQLQ QAIRLLQLST LDLQQEIQEA LESNPMLERQ EDGDDFDNSD PLADGAEQAA SAPQESPLQE SATPSVESLD DDQWSERIPS ELPVDTAWED IYQTSASSLP SNDDDEWDFT ARTSSGESLH SHLLWQVNLA PMSDTDRMIA VTIIDSINND GYLEESLEEI LAAIDPELDV ELDEVEVVLR RIQQLEPAGI GARNLRECLL LQLRQLPSTT PWLNEALRLV SDYLDLLGGR DYSQLMRRMK LKEDELRQVI ELIQCLHPRP GSQIESSEAE YIVPDVIVRK DNERWLVELN QEAMPRLRVN ATYAGMVRRA DSSADNTFMR NQLQEARWFI KSLQSRNETL MKVATQIVEH QRGFLDYGEE AMKPLVLHDI AEAVGMHEST ISRVTTQKYM HTPRGIFELK YFFSSHVSTA EGGECSSTAI RAIIKKLVAA ENAKKPLSDS KIAGLLEAQG IQVARRTVAK YRESLGIAPS SERKRLV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Plays a role in the regulation of many virulence factors, motility, quorum sensing, mucoidy, a general mechanism for maintaining lytic phage in populations of bacteria, and biofilm formation. Positively controls the T6 secretion system by directly binding to the promoter regions of hcpA and hcpB genes, leading to their expression. Thereby, allows to colonize several hosts efficiently including mammals, insects, nematodes and plants.
  • 基因功能参考文献：
    1. RpoN interacts with pqsA, pqsE, pqsH, and rhlR in response to antibiotic stress.RpoN negatively regulates the expression of Pseudomonas quinolone signal. PMID: 27431228
    2. Together, these data strongly suggest that KinB regulates virulence factors important for the development of acute pneumonia and conversion to mucoidy via RpoN. PMID: 22210761
    3. RpoN negatively regulates aceA activity. PMID: 20093293
  • 蛋白家族：
    Sigma-54 factor family
  • 数据库链接：

    KEGG: pae:PA4462

    STRING: 208964.PA4462