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  4. Recombinant Rat A-kinase anchor protein 8 (Akap8)

Recombinant Rat A-kinase anchor protein 8 (Akap8)

  • 货号：
    CSB-YP730871RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP730871RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP730871RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP730871RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP730871RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Akap8; Akap95A-kinase anchor protein 8; AKAP-8; A-kinase anchor protein 95 kDa; AKAP 95
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-687
  • 氨基酸序列
    MEQSYGGYGA WSAGPANTQG TYGSGVASWQ GYENYSYYNA QNTSVPTGTP YSYGPASWEA TKASDGGLAA GSSAMHVASF APEPCTDNSD SLIAKINQRL DMLSKEGGRG GISSGGEGMQ DRDSSFRFQP YESYDSRPCM PEHTPYRPSY SYDYDFDLGT DRNGSFGGTF NDCRDPTPER GALDGFLRGR GQGRFQDRSN SSTFIRSDPF MPPSASSEPL STTWSELNYM GGRGLGGPST NRPPPSLFSQ SMAPDYSMMG MQGVGGFGGT MPYGCGRSQT RIRDWPRRRG FERFGPDNMG RKRKPFPLYE EPDAKLARAD SEGDLSENDD GAGDLRSGDE EFRGEDDLCD SRKQRGEKED EDEDVKKRRE KQRRRDRMRD RAADRIQFAC SVCKFRSFED EEIQKHLQSK FHKETLRFIS TKLPDKTVEF LQEYIINRNK KIEKRRQELL EKESPKPKPD PFKGIGQEHF FKRIEAAHCL ACDMLIPAQH QLLQRHLHSV DHNHNRRLAA EQFKKTSLHV AKSVLNNKHI VKMLEKYLKG EDPFVNETAD LETEGDENLG EEKETPEEVA AEVLAEVITA AVKAVEGDGE PAAEHSDVLA EVEGPVDTAE AGSDSHTGKL LEEQTCETAS ETRNMEDMAR GEAAEARNEA AVPAAAAGSP VPVIAIPGIL EDELEQTDAE AKDTPTE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II). Acts as an anchor for a PKA-signaling complex onto mitotic chromosomes, which is required for maintenance of chromosomes in a condensed form throughout mitosis. Recruits condensin complex subunit NCAPD2 to chromosomes required for chromatin condensation; the function appears to be independent from PKA-anchoring. May help to deliver cyclin D/E to CDK4 to facilitate cell cycle progression. Required for cell cycle G2/M transition and histone deacetylation during mitosis. In mitotic cells recruits HDAC3 to the vicinity of chromatin leading to deacetylation and subsequent phosphorylation at 'Ser-10' of histone H3; in this function may act redundantly with AKAP8L. Involved in nuclear retention of RPS6KA1 upon ERK activation thus inducing cell proliferation. May be involved in regulation of DNA replication by acting as scaffold for MCM2. Enhances HMT activity of the KMT2 family MLL4/WBP7 complex and is involved in transcriptional regulation. In a teratocarcinoma cell line is involved in retinoic acid-mediated induction of developmental genes implicating H3 'Lys-4' methylation. May be involved in recruitment of active CASP3 to the nucleus in apoptotic cells. May act as a carrier protein of GJA1 for its transport to the nucleus. May play a repressive role in the regulation of rDNA transcription. Preferentially binds GC-rich DNA in vitro. In cells, associates with ribosomal RNA (rRNA) chromatin, preferentially with rRNA promoter and transcribed regions. Involved in modulation of Toll-like receptor signaling. Required for the cAMP-dependent suppression of TNF-alpha in early stages of LPS-induced macrophage activation; the function probably implicates targeting of PKA to NFKB1.
  • 基因功能参考文献：
    1. new insights into how AKAP95 orchestrates cell cycle progression. PMID: 22130794
    2. lactate dehydrogenase subunit A mRNA stability is regulated by Cyclic AMP and AKAP-mediated targeting of protein kinase A PMID: 15878851
    3. Used cell permeable peptide to disrupt endogenous protein kinase A R2alpha (pkar2a)-AKAP95 complexes. PMID: 19073898
  • 亚细胞定位：
    Nucleus matrix. Nucleus, nucleolus. Cytoplasm.
  • 蛋白家族：
    AKAP95 family
  • 组织特异性：
    Widely expressed. The protein has been detected in liver, fibroblasts, granulosa, myoblast, lymphoma and Sertoli cells.
  • 数据库链接：

    KEGG: rno:116633

    STRING: 10116.ENSRNOP00000008827

    UniGene: Rn.10248