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  4. Recombinant Rat Achaete-scute homolog 1 (Ascl1)

Recombinant Rat Achaete-scute homolog 1 (Ascl1)

  • 货号：
    CSB-YP002199RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP002199RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP002199RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP002199RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP002199RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ascl1; Ash1; Mash-1; Achaete-scute homolog 1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-233
  • 氨基酸序列
    MESSGKMESG AGQQPQPPQP FLPPAACFFA TAAAAAAAAA AAAAQSAQQQ QQQQAPQQQA PQLSPVADGQ PSGGGHKSAA KQVKRQRSSS PELMRCKRRL NFSGFGYSLP QQQPAAVARR NERERNRVKL VNLGFATLRE HVPNGAANKK MSKVETLRSA VEYIRALQQL LDEHDAVSAA FQAGVLSPTI SPNYSNDLNS MAGSPVSSYS SDEGSYDPLS PEEQELLDFT NWF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that plays a key role in neuronal differentiation: acts as a pioneer transcription factor, accessing closed chromatin to allow other factors to bind and activate neural pathways. Directly binds the E box motif (5'-CANNTG-3') on promoters and promotes transcription of neuronal genes. The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro. Plays a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Acts synergistically with FOXN4 to specify the identity of V2b neurons rather than V2a from bipotential p2 progenitors during spinal cord neurogenesis, probably through DLL4-NOTCH signaling activation. Involved in the regulation of neuroendocrine cell development in the glandular stomach.
  • 基因功能参考文献：
    1. Data found that Ascl1 promoted tangential migration along the ventricular zone/subventricular zone (VZ/SVZ) and intermediate zone (IZ) of the dorsal telencephalon. Also, Ascl1 induces Ephb2 expression in the telencephalon. PMID: 28276447
    2. miR-34a appears to play an important role in the process of liver fibrosis by targeting ACSL1 and may show promise as a therapeutic molecular target for hepatic fibrosis PMID: 26437572
    3. Data demonstrate that MASH1/Ascl1a is a fundamental regulator of axonal growth across vertebrates and can induce modifications to the intrinsic state of neurons to promote functional regeneration in response to central nervous system injury. PMID: 25751153
    4. data suggest that a prominent down-regulation of hnRNP-A2/B1 during hypoxia is associated with the post-transcriptional suppression of hASH1 synthesis. PMID: 25124043
    5. This study demonistrated that over-expression of Mash1 can improve GABAergic differentiation of bone marrow mesenchymal stem cells. PMID: 24144723
    6. after FF transection, Mash1 expression in the deafferented hippocampus increased and might play an important role in inducing local progenitors to differentiate into neurons. PMID: 22583763
    7. TLX directly targets and activates the MASH1 promoter through interaction with Sp1, recruiting co-activators whereas dismissing the co-repressor HDAC4. PMID: 20599619
    8. Differentiation of cardiac ganglionic cells is affected, after RETINOIC ACID treatment, by the down-regulation of Mash-1. PMID: 11803116
    9. In cultures of neural tube-derived, primitive PNS progenitors NGNs promote expression of sensory markers and MASH1 that of autonomic markers. PMID: 11923194
    10. The expression of MASH 1 mRNA is increased in olfactory tissure from vitamin A deficiency rats PMID: 12555267
    11. The level of Mash1 mRNA increased on day 1 and then returned to the basal level by 3 days after ischemia. PMID: 15854743
    12. The Mash1 contributes to the generation of DA neurons in cooperation with Nurr1 in the developing midbrain whereas atonal-related bHLH genes inhibit the process. PMID: 16723737
    13. cooperation between Mash1 and Olig2 is necessary for neural stem/progenitor cells to develop into fully mature neurons and that down-regulation of Olig2 by Abeta in Mash1-overexpressing cells switches the cell fate to death. PMID: 17488716
    14. The present study suggests that the majority of the Mash1+ cells in the SGZ may be transit-amplifying neural progenitor cells. PMID: 17727826
    15. increased GABA neuron yield by Ngn2 and decreased yield by Mash1 were shown in the precursors of posterior brain regions PMID: 17936272
    16. Ascl1 is required for oligodendrocyte development in the spinal cord. PMID: 18287202
    17. Upon transplantation into adult rat brains, Mash1-expressing NPCs yielded large grafts enriched with neurons compared to control LacZ-transduced NPCs. PMID: 18781144
    18. Improved translation efficiency of ASH1 mRNA by FMRP may represent an important regulatory switch in neuronal differentiation. PMID: 19097999

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  • 亚细胞定位：
    Nucleus.
  • 组织特异性：
    Developing CNS and PNS at embryonic and postnatal stages.
  • 数据库链接：

    KEGG: rno:64186

    STRING: 10116.ENSRNOP00000005674

    UniGene: Rn.32936