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  4. Recombinant Rat Acid-sensing ion channel 1 (Asic1), partial

Recombinant Rat Acid-sensing ion channel 1 (Asic1), partial

  • 中文名称：
    Recombinant Rat Acid-sensing ion channel 1(Asic1),partial,Yeast
  • 货号：
    CSB-YP001142RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Acid-sensing ion channel 1(Asic1),partial,Yeast
  • 货号：
    CSB-EP001142RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Acid-sensing ion channel 1(Asic1),partial,Yeast
  • 货号：
    CSB-EP001142RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Acid-sensing ion channel 1(Asic1),partial,Yeast
  • 货号：
    CSB-BP001142RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Acid-sensing ion channel 1(Asic1),partial,Yeast
  • 货号：
    CSB-MP001142RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Asic1
  • Uniprot No.：
  • 别名：
    Asic1; Accn2; Bnac2; Acid-sensing ion channel 1; ASIC1; Amiloride-sensitive cation channel 2; neuronal; Brain sodium channel 2; BNaC2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Proton-gated sodium channel; it is activated by a drop of the extracellular pH and then becomes rapidly desensitized. Generates a biphasic current with a fast inactivating and a slow sustained phase. Has high selectivity for sodium ions and can also transport lithium ions with high efficiency. Can also transport potassium ions, but with lower efficiency. It is nearly impermeable to the larger rubidium and cesium ions. Isoform 3 discrimates stronger than isoform 1 between monovalent cations. Isoform 3 can flux Ca(2+) while isoform 1 cannot. Heteromeric channels composed of isoform 2 and isoform 3 are active but have a lower pH-sensitivity. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties.
  • 基因功能参考文献：
    1. These results show a crucial role of ASIC1a channels in the BLA in pain and anxiety-related behaviours during arthritis. PMID: 28321113
    2. Asic1 was found to be localized in astrocytes and microglia in the nucleus tractus solitarii. PMID: 28825196
    3. ASIC1a inhibits the expression of MMP-2/9, GAG, HYP, and TIMP-1/2 by the Ca(2+)-dependent P38 MAPK/c-jun and ERK/c-fos signaling pathways. PMID: 29086909
    4. these findings suggested that both inhibition and silencing of autophagy could enhance ASIC1a-mediated apoptosis in rat articular chondrocytes, and therefore, autophagy is likely to be a new mechanism involved in ASIC1a-mediated apoptosis of articular chondrocytes during the pathogenesis of RA. PMID: 29273849
    5. these findings suggested that AMPK/FoxO3a axis plays an important role in ASIC1a-mediated autophagy in rat articular chondrocytes, which may provide novel mechanistic insight into ASIC1a effects on autophagy. PMID: 29019932
    6. Amiloride inhibited pilocarpine-induced seizures by deactivating ASIC1a/ASIC3 channels. PMID: 26823770
    7. The overload of intracellular [Ca(2+)] caused by ASIC1a activation could be one mechanism for the aggravation of focal cerebral ischemia in diabetes. PMID: 26722526
    8. ASIC1a activation by extracellular acid induces extracellular matrix metabolism in rat endplate chondrocytes by increasing matrix metalloproteinase expression and activity through NF-kappaB transcriptional activity. PMID: 26384841
    9. The ASIC1 signal pathway is involved in extracellular acidosis-induced cortical neuronal injury. PMID: 26715049
    10. Molecular dynamics and functional studies define a hot spot of crystal contacts essential for PcTx1 inhibition of acid-sensing ion channel 1a PMID: 26248594
    11. PKA and protein interacting with C kinase 1/calcineurin differentially regulate ASIC1-mediated store-operated Ca(2+) entry PMID: 26702130
    12. Acid-sensing ion channel 1a drives AMPA receptor plasticity following ischaemia and acidosis in hippocampal CA1 neurons PMID: 26174503
    13. These data provide information on the structure and on the pharmacophore for ASIC channel inhibition by mambalgins that could have therapeutic value against pain and probably other neurological disorders. PMID: 26680001
    14. These data indicate that ASIC1a plays an important role in diabetes complication liver fibrosis. PMID: 26562527
    15. Studies identified that ASIC1a was expressed in rat liver and hepatic stellate cells and provided a strong evidence for the involvement of the ASIC1a in the progression of hepatic fibrosis. PMID: 24939363
    16. Results demonstrate that chloroquine affects vision conduction by directly modifying the kinetics of ASIC1a. PMID: 24821433
    17. ASIC1a activation in endplate chondrocytes may trigger Ca(2+)-dependent proteases activity and signaling, which leads to apoptosis of endplate chondrocytes in IVDs PMID: 24261866
    18. Neuroprotective effects of ischemic preconditioning on global brain ischemia through up-regulation of acid-sensing ion channel 2a. PMID: 20162006
    19. Binding site and inhibitory mechanism of the mambalgin-2 pain-relieving peptide on acid-sensing ion channel 1a. PMID: 24695733
    20. In the rat BLA, ASIC1a has an inhibitory and anxiolytic function. PMID: 24573273
    21. findings show that ASIC1a-mediated calcium entry plays a critical role in osteoclastogenesis by regulating activation of the NFATc1 PMID: 23994523
    22. Our results showed that both TRPM and ASIC channels were expressed in the brain and that ginsenoside neuroprotection following cerebral ischemia may be at least due to its effects on the expression of TRPM7, ASIC1a and ASIC2a. PMID: 22231470
    23. the orexin neuron in the LH can exert an excitation on respiration via ASIC1 during local acidosis. PMID: 22792205
    24. Thus, our results reveal a novel mechanism underlying central sensitization and pain hypersensitivity, and reinforce the critical role of ASIC1a channels in these processes. PMID: 22553040
    25. This study supports a novel role of ASIC1 in elevated receptor-stimulated vasoconstriction following chronic hypoxia which is likely mediated through increased ASIC1 expression and store-operated Ca(2 ) entry. PMID: 22205392
    26. ASIC1a expression in hippocampus is increased by chronic lead exposure. PMID: 19257932
    27. These data suggest that different ENaC/ASIC1 subunits interact and could combine to form a hybrid channel that likely underlies the amiloride-sensitive current seen in human glioma cells. PMID: 21346156
    28. NG2 cells of the hippocampus express high density of Ca2+-permeable ASIC1a. PMID: 20844750
    29. Asn415 in the beta11-beta12 linker decreases proton-dependent desensitization of ASIC1. PMID: 20675379
    30. Findings show that increased [Ca(2+)]i, mediated via ASIC1a, might contribute to acidosis-induced articular chondrocyte injury. PMID: 20179994
    31. Examined ASIC1 from two evolutionary distant species, lamprey and rat, to identify structural elements and residues that account for the functional differences of these channels. PMID: 20427715
    32. analysis of the Extracellular chloride modulates the desensitization kinetics of acid-sensing ion channel 1a (ASIC1a) PMID: 20385551
    33. Activation of acid-sensing ion channel 1a (ASIC1a) by surface trafficking. PMID: 20185828
    34. mRNA expression of ASIC1b was significantly higher in SHR than in WKY carotid bodies before hypertension developed. PMID: 20019330
    35. The ASICs in PC12 cells were selective for Na(+) with significant Ca(2+) permeability. PMID: 11976391
    36. Broad expression of ASIC1 in many areas of adult rat brain including cerebral cortex, hippocampus and cerebellum. Within cells, ASIC1 was found predominantly throughout soma and along the branches of axons and dendrites. PMID: 12509480
    37. the extracellular domain determines the kinetics of desensitization in acid-sensitive ion channel 1 PMID: 12947112
    38. p11, already known to traffic members of the voltage-gated sodium and potassium channel families as well as transient receptor potential and chloride channels, also plays a selective role in enhancing ASIC1a functional expression PMID: 16169854
    39. For ASIC1b, PcTx1 binds most tightly to the open state, promoting opening, whereas for ASIC1a, it binds most tightly to the open and the desensitized state, promoting desensitization. PMID: 16505147
    40. Our study indicates that ASIC1a is an important channel in normal retinal activity. PMID: 16723538
    41. ASIC2 is expressed in the distinct neurochemical population of sensory neurons as well as satellite cells, and that peripheral axotomy induced marked reductions in ASIC2 in neurons. PMID: 16949762
    42. tachyphylaxis depends on H+ permeating through ASIC1a; tachyphylaxis is attenuated by extracellular Ca2+, and is probably linked to Ca2+ permeability of ASIC1a PMID: 17204502
    43. ASICs may contribute to chemotransduction of low pH by carotid body chemoreceptors PMID: 17872465
    44. Vascular smooth muscle ASIC1 is required for normal cell migration. PMID: 17936312
    45. Analysis of the action of diclofenac and ibuprofen on activation and desensitization of ASICs showed that diclofenac but not ibuprofen shifted the steady-state desensitization curve to more alkaline pH values. PMID: 18410516
    46. Oligodendrocyte lineage cells expressing ASIC1a provide a means for an acid shift in the central nervous system extracellular pH. PMID: 18452213
    47. These findings suggest that ASIC3 is associated with hyperalgesia in response to a chemical stimulus in the L5 SNL rat model. PMID: 18534561
    48. data establish a unique role for ASIC1 in mediating store operated Ca+ entry in pulmonary vascular smooth muscle and provide new insight into mechanisms of VSM Ca(2+) entry and pulmonary vasoregulation PMID: 19482897

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Amiloride-sensitive sodium channel (TC 1.A.6) family, ASIC1 subfamily
  • 组织特异性：
    Expressed in dorsal root ganglia and sciatic nerve (at protein level). Widely distributed throughout the brain. Expressed in olfactory bulb, neo and allocortical regions, dentate granule cells, pyramidal cells of CA1-CA3 subfields of the hippocampal forma
  • 数据库链接：

    KEGG: rno:79123

    UniGene: Rn.37385