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  4. Recombinant Rat Activity-regulated cytoskeleton-associated protein (Arc)

Recombinant Rat Activity-regulated cytoskeleton-associated protein (Arc)

  • 中文名称：
    Recombinant Rat Activity-regulated cytoskeleton-associated protein(Arc),Yeast
  • 货号：
    CSB-YP001981RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Activity-regulated cytoskeleton-associated protein(Arc),Yeast
  • 货号：
    CSB-EP001981RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Activity-regulated cytoskeleton-associated protein(Arc),Yeast
  • 货号：
    CSB-EP001981RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Activity-regulated cytoskeleton-associated protein(Arc),Yeast
  • 货号：
    CSB-BP001981RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Activity-regulated cytoskeleton-associated protein(Arc),Yeast
  • 货号：
    CSB-MP001981RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Arc
  • Uniprot No.：
  • 别名：
    ArcActivity-regulated cytoskeleton-associated protein; Activity-regulated gene 3.1 protein; ARC/ARG3.1; Arg3.1
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-396
  • 氨基酸序列
    MELDHMTTGG LHAYPAPRGG PAAKPNVILQ IGKCRAEMLE HVRRTHRHLL TEVSKQVERE LKGLHRSVGK LENNLDGYVP TGDSQRWKKS IKACLCRCQE TIANLERWVK REMHVWREVF YRLERWADRL ESMGGKYPVG SEPARHTVSV GVGGPEPYCQ EADGYDYTVS PYAITPPPAA GELPEQESVG AQQYQSWVPG EDGQPSPGVD TQIFEDPREF LSHLEEYLRQ VGGSEEYWLS QIQNHMNGPA KKWWEFKQGS VKNWVEFKKE FLQYSEGTLS REAIQRELDL PQKQGEPLDQ FLWRKRDLYQ TLYVDAEEEE IIQYVVGTLQ PKFKRFLRHP LPKTLEQLIQ RGMEVQDGLE QAAEPSVTPL PTEDETEALT PALTSESVAS DRTQPE
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Master regulator of synaptic plasticity that self-assembles into virion-like capsids that encapsulate RNAs and mediate intercellular RNA transfer in the nervous system. ARC protein is released from neurons in extracellular vesicles that mediate the transfer of ARC mRNA into new target cells, where ARC mRNA can undergo activity-dependent translation. ARC capsids are endocytosed and are able to transfer ARC mRNA into the cytoplasm of neurons. Acts as a key regulator of synaptic plasticity: required for protein synthesis-dependent forms of long-term potentiation (LTP) and depression (LTD) and for the formation of long-term memory. Regulates synaptic plasticity by promoting endocytosis of AMPA receptors (AMPARs) in response to synaptic activity: this endocytic pathway maintains levels of surface AMPARs in response to chronic changes in neuronal activity through synaptic scaling, thereby contributing to neuronal homeostasis. Acts as a postsynaptic mediator of activity-dependent synapse elimination in the developing cerebellum by mediating elimination of surplus climbing fiber synapses. Accumulates at weaker synapses, probably to prevent their undesired enhancement. This suggests that ARC-containing virion-like capsids may be required to eliminate synaptic material. Required to transduce experience into long-lasting changes in visual cortex plasticity and for long-term memory. Involved in postsynaptic trafficking and processing of amyloid-beta A4 (APP) via interaction with PSEN1. In addition to its role in synapses, also involved in the regulation of the immune system: specifically expressed in skin-migratory dendritic cells and regulates fast dendritic cell migration, thereby regulating T-cell activation.
  • 基因功能参考文献：
    1. In dissociated rat cortical neurons, H2A.Z.1 and H2A.Z.2 regulate basal expression of largely nonoverlapping gene sets, including genes that code for several synaptic proteins. In response to neuronal activity, rapid transcription of our model gene Arc is impaired by depletion of H2A.Z.2, but not H2A.Z.1. This impairment is partially rescued by codepletion of the H2A.Z chaperone, ANP32E. PMID: 28856239
    2. Results suggest that Arc-dependent plasticity during taste learning is required within distinct structures of the medial temporal lobe, depending on the emotional valence of the memory trace. PMID: 29326059
    3. This study demonstrated that the Arc expression in auditory and visual cortices after hearing in rats. PMID: 28472857
    4. Findings shed further light on the molecular and cellular mechanisms for the formation of fear extinction memory with Arc centrally placed to orchestrate the consolidation of extinction: Arc gates extinction at recall, with high, sustained levels of Arc (through CNQX administration) constraining extinction and decreases in Arc (through Arc antisense administration) facilitating premature extinction. PMID: 28716957
    5. We conclude that an increased Arc expression may be related to enhanced synaptic plasticity in the ventral striatum, suggesting that it may be one of the physiological substrates of enhanced learning. PMID: 28185871
    6. Hippocampal but not striatal Arc expression decreases with task experience in spatial navigation in rats. PMID: 26976088
    7. No changes in either Arc or GAD protein expression were observed in primary auditory cortex in the immediate post-noise exposure period, confirming other reports that auditory cortical plasticity may not occur until later in the development of tinnitus. PMID: 27702572
    8. observations support a model in which stalled ribosomes are reactivated to rapidly generate Arc protein. PMID: 27397520
    9. These findings reveal a more complete cholinergic signaling that is responsible for carbachol-induced Arc protein expression. PMID: 26895748
    10. Maternal stress during pregnancy affects Arc expression in offspring' hippocampus. PMID: 27474832
    11. these findings link the pro-neurogenic effects of acute BDNF infusion to induction of Arc/Arg3.1-dependent long-term potentiation in the adult rodent dentate gyrus. PMID: 26888068
    12. Intracranial self-stimulation potentiates proto-oncogene proteins c-fos induction across active avoidance conditioning conditions along with increases of activity regulated cytoskeletal-associated protein protein levels in the retrosplenial cortex. PMID: 26774022
    13. Female rats which had been pre-exposed to prenatal stress, displayed an "amplified" Arc upregulation in response to postnatal stress compared to unstressed controls, which may reflect a "sensitization" effect of prenatal stress. PMID: 25239865
    14. The targeted knockdown of Arc in the brain results in a non-recoverable retrograde amnesia. PMID: 26238574
    15. Study demonstrated that ARC is a specific, quantifiable and input dependent molecular marker of circuit activation which can serve to quantify the impact of brain injury and subsequent rehabilitation on whisker sensation PMID: 25682931
    16. Recently-generated granule cells showed a robust sustained Arc expression following a discrete behavioral experience. PMID: 26219984
    17. Abstinence from developmental exposure to cocaine is associated with alterations in the finely tuned mechanisms that regulate Arc/Arg3.1 expression. PMID: 24810662
    18. Arc mRNA was significantly decreased in the dorsal striatum of addiction vulnerable rats, and this reduction was restricted to the dorsomedial and not dorsolateral striatum. PMID: 25646592
    19. Results showed that the expression of Arc, but not zif268, was significantly increased in the cerebellum following motor skill learning PMID: 25249385
    20. BDNF activated the Arc proximal promoter, whereas class I histone deacetylases (HDACs) inhibited this activation. PMID: 25623071
    21. Decreased expression of Arc and Egr-1 might be involved with instability of synaptic plasticity in tinnitus. PMID: 24704997
    22. Deficits in Arc expression in the amygdala during alcohol withdrawal are reversed following treatment with HDAC inhibitors. PMID: 24103311
    23. Collectively, these results suggest that Arc/Arg3.1 protein plays an important functional role in both the initial acquisition of hippocampal-dependent memory and the reconsolidation of these memories after recall. PMID: 23872190
    24. NMDA- and dopamine D1 receptor-dependent Arc expression are important in reward-enforced, drug-seeking behavior. PMID: 23708554
    25. The data of this study reveal a novel mechanism for controlling mRNA distribution within dendrites and highlight activity-dependent mRNA degradation as a regulatory process involved in synaptic plasticity. PMID: 24671994
    26. findings suggest a link between motor cortex Arc expression and motor skill learning in rats PMID: 23876329
    27. The retrieval of contextual memories increases Arc in the amygdala and hippocampus. PMID: 22945419
    28. Our results demonstrate that the IEGs, egr1 and arc, may significantly contribute to synaptic reorganization induced by the kindling of seizures PMID: 23744421
    29. Arc/Arg3.1 lateralization inversely correlates with taste familiarity in insular cortex. PMID: 23843540
    30. Arg3.1 mediates the final stage of climbing fiber synapse elimination in the developing cerebellum. PMID: 23791196
    31. Inner hair cell ribbon loss (deafferentation) leads to tinnitus when auditory brainstem response functions remain reduced and Arc is not mobilized. PMID: 23154938
    32. This study demonistrated that isolation rearing alters ARC activation in the mPFC produced by exposure to a novel conspecific, in addition to changing social behavior, and that these effects depend in part on sex. PMID: 22982514
    33. Arc protein expression is increased for over four hours in the hippocampus, demonstrating DNA methylation as a regulator of electroconvulsive stimulation (ECS)-induced gene expression. PMID: 22613772
    34. Behavior-induced cell activity across the CA1 hippocampal axis uses Arc mRNA imaging methodology to compare and monitor adult and old rats in two different field remapping conditions. PMID: 23426670
    35. Data from experimental model of developmentally-induced hypothyroidism suggest that hippocampus exhibits down-regulation of Arc and Egr1 (early growth response protein 1) compared with control; such mice exhibit deficits in spatial learning/memory. PMID: 23079472
    36. Maze-running rats expressed Arc in more pyramidal cells than caged-controls. The pattern of Arc expression depended upon navigational strategy (place or response). PMID: 22573703
    37. these data demonstrate a unique kinetic profile for Arc transcription in hippocampal granule neurons following behavior that is not observed in other cell types. PMID: 23345235
    38. These observations imply the existence of a microdomain at the spine base where Arc mRNA docks in association with a previously unknown mRNA-binding structural element. PMID: 22350812
    39. Arc is necessary for long-term memory formation of two types of learning tasks and is one of the PRPs that can be shared between them when animals are trained in both open field exploration and inhibitory avoidance. PMID: 22683463
    40. Arc is regulated by multiple miRNAs and modulated by specific miRNA pairs in vitro PMID: 22844515
    41. Arc signaling in the dorsomedial striatum is necessary for consolidation of reversal learning associated with a METH-induced change in neural substrates mediating such reversal learning. PMID: 22071872
    42. Fewer granule cells in the aged fascia dentata transcribe arc 8 hours after spatial exploration; this decrease is correlated with impaired spatial memory. PMID: 20850902
    43. neurons that were recently active during spatial exploration are preferentially recruited into further Arc expression during subsequent rest PMID: 22535445
    44. Increased synaptic Arc/Arg3.1 expression contributes to aversive memory formation by regulating synaptic AMPA receptor (AMPAR) endocytosis. PMID: 22933785
    45. analysis of dynamic, multifaceted control of Arc synthesis during mAchR signaling PMID: 22584581
    46. These data suggest that cholinergic denervation of hippocampus leads to deficits in muscarinic-dependent induction of Arc and a subsequent impairment of spatial memory acquisition. PMID: 20865740
    47. Arc transcription is reduced within the aged hippocampus compared to the adult hippocampus during both "off line" periods of rest, and following spatial behavior. PMID: 20189687
    48. Study shows targeting of Arc to inactive synapses via a high-affinity interaction with CaMKIIbeta that is not bound to calmodulin. PMID: 22579289
    49. the importance of intracellular mGluR5 in the cascade of events associated with sustained synaptic transmission PMID: 22179607
    50. In CA1 there was a persistent increase in arc mRNA level during long-term potentiation, but during long-term depression, arc mRNA level first was decreased & then transiently increased relative to control level. PMID: 20824728

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  • 亚细胞定位：
    Extracellular vesicle membrane; Lipid-anchor. Cell junction, synapse, postsynaptic cell membrane; Lipid-anchor. Cell junction, synapse. Cell junction, synapse, postsynaptic density. Early endosome membrane. Cell projection, dendrite. Cytoplasm, cytoskeleton. Cytoplasm, cell cortex. Cell projection, dendritic spine. Cytoplasmic vesicle, secretory vesicle, acrosome.
  • 蛋白家族：
    ARC/ARG3.1 family
  • 组织特异性：
    Expressed exclusively in certain parts of the brain including cortex and molecular layer of the hippocampus. Typically expressed at high level in a minority of neurons. Basal expression higher in cortex than in hippocampus, highest in visual cortex.
  • 数据库链接：

    KEGG: rno:54323

    STRING: 10116.ENSRNOP00000063719

    UniGene: Rn.10086