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  4. Recombinant Rat Aldose reductase (Akr1b1)

Recombinant Rat Aldose reductase (Akr1b1)

  • 货号：
    CSB-YP001539RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001539RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP001539RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001539RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001539RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Akr1b1
  • Uniprot No.：
  • 别名：
    Akr1b1; Akr1b4; Aldr1Aldo-keto reductase family 1 member B1; EC 1.1.1.21; EC 1.1.1.300; EC 1.1.1.372; EC 1.1.1.54; Aldehyde reductase; Aldose reductase; AR
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-316
  • 氨基酸序列
    ASHLELNNG TKMPTLGLGT WKSPPGQVTE AVKVAIDMGY RHIDCAQVYQ NEKEVGVALQ EKLKEQVVKR QDLFIVSKLW CTFHDQSMVK GACQKTLSDL QLDYLDLYLI HWPTGFKPGP DYFPLDASGN VIPSDTDFVD TWTAMEQLVD EGLVKAIGVS NFNPLQIERI LNKPGLKYKP AVNQIECHPY LTQEKLIEYC HCKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKEIAAK YNKTTAQVLI RFPIQRNLVV IPKSVTPARI AENFKVFDFE LSNEDMATLL SYNRNWRVCA LMSCAKHKDY PFHAEV
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.; Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal. Catalyzes the reduction of diverse phospholipid aldehydes such as 1-palmitoyl-2-(5-oxovaleroyl)-sn -glycero-3-phosphoethanolamin (POVPC) and related phospholipid aldehydes that are generated from the oxydation of phosphotidylcholine and phosphatdyleethanolamides. Plays a role in detoxifying dietary and lipid-derived unsaturated carbonyls, such as crotonaldehyde, 4-hydroxynonenal, trans-2-hexenal, trans-2,4-hexadienal and their glutathione-conjugates carbonyls (GS-carbonyls).
  • 基因功能参考文献：
    1. AKR1B1 played a vital role in astrocyte proliferation and energy metabolism induced by spinal cord injury. PMID: 29948725
    2. Coincubation of cells with epalrestat prevented the signaling changes and renal cell injury induced by high glucose. Thus, aldose reductase inhibition represents a potential therapeutic strategy to prevent Diabetic nephropathy PMID: 28386557
    3. The role of aldose reductase in the development of diabetic nephropathy: Aldose reductase was upregulated in diabetic rat renal tissues. PMID: 26056446
    4. The presented results showed the ability of CMTI to affect the polyol pathway in diabetic rats in vivo. PMID: 26291727
    5. Activation of PI3K by 15-Deoxy-delta(12,14)-prostaglandin J2 increases the expression of aldose reductase through Nrf2. PMID: 24180671
    6. Data suggest that aldose reductase (AR) plays a mediatory role in ocular neovascularization as seen in diabetic retinopathy; inhibition of AR may have therapeutic potential in diabetic retinopathy. PMID: 22658411
    7. analysis of the inhibition of aldose reductase by Gentiana lutea extracts PMID: 22844269
    8. Data suggest that inhibition of AR activity in lens prevents cataract development in diabetes (using chlorogenic acid as AR inhibitor in galactose-fed rat model). AR protein is highly expressed in lens epithelial cells/cortical fibers in this model. PMID: 21656371
    9. the significance of AKR1B1 in the mediation of sugar-induced lens opacification PMID: 21376710
    10. Aldose Reductase may play a key in intestinal ischemic protection PMID: 21136599
    11. The present findings suggest that AR participates in estrogen's deleterious action on post-ischemic neuropathology in diabetics by promoting inflammation. PMID: 20417192
    12. Data show that inhibition of AR may be a useful therapeutic approach in preventing vascular complications. PMID: 20308528
    13. Findings indicate that aldose reductase is a component of ischemic injury and that pharmacological inhibitors of aldose reductase present a novel adjunctive approach for protecting ischemic hearts. PMID: 11772943
    14. Gene expression of enzymes comprising the polyol pathway in various rat tissues determined by the competitive RT-PCR method. PMID: 11855672
    15. Mediates mitogenic signaling in vascular smooth muscle cells PMID: 12063254
    16. Aldose reductase is induced by hyperosmotic stress and mediates cardiomyocyte apoptosis PMID: 12881532
    17. Aldose reductase prevents glucose-induced stimulation of vascular smooth muscle cell growth in culture and in situ. PMID: 16567509
    18. Several anthraquinones isolated from Cassia inhibited Alr of the crystalline lens. PMID: 17978503
    19. Triiodothyronine regulates AKR1B1 gene expression via a thyroxine receptor response element-dependant mechanism and associates liver cancer. PMID: 19422879

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Aldo/keto reductase family
  • 数据库链接：

    KEGG: rno:24192

    STRING: 10116.ENSRNOP00000012879

    UniGene: Rn.107801