Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Alpha-crystallin B chain (Cryab)

Recombinant Rat Alpha-crystallin B chain (Cryab)

  • 货号：
    CSB-YP006008RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006008RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006008RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006008RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006008RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cryab; Alpha-crystallin B chain; Alpha(B)-crystallin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-175
  • 氨基酸序列
    MDIAIHHPWI RRPFFPFHSP SRLFDQFFGE HLLESDLFST ATSLSPFYLR PPSFLRAPSW IDTGLSEMRM EKDRFSVNLD VKHFSPEELK VKVLGDVIEV HGKHEERQDE HGFISREFHR KYRIPADVDP LTITSSLSSD GVLTVNGPRK QASGPERTIP ITREEKPAVT AAPKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions.
  • 基因功能参考文献：
    1. CryaB expression in themyocardium increases in aged rats. Manifestation of cardiomyopathy occurs against the background of increased phosphorylation of CryaB (on Ser59) and its translocation to striated sarcomeres. PMID: 28502773
    2. These data suggest that among all small heat shock proteins, HspB1 and alpha(B)-crystallin might be most important in the neuronal stress response to ischemia/reperfusion injury in the brain since their protein levels were upregulated to the highest extent after transient middle cerebral artery occlusion. PMID: 28425051
    3. HspB5 prevented heat shock-induced rarefaction of dendrites. PMID: 27085702
    4. alphaB-crystallin protects rat articular chondrocytes against CK2 inhibition-induced apoptosis. PMID: 27851782
    5. results revealed a new role for CryAB, as a modulator of Shp2 phosphatase activity during a functionally relevant stimulus in cardiomyocytes. PMID: 27264546
    6. These results were confirmed by analysis with STRING 9.1 software, which indicated that Hsp27 and aB-crystallin are co-expressed in rat myocardial cells PMID: 26708692
    7. data suggest that HspB1 and HspB5 might be of protective value for retinal ganglion cells after optic nerve injury and that this process might be regulated by phosphorylation at serine 86 of HspB1 and serine 19 and serine 45 of HspB5. PMID: 26475352
    8. alphaB-crystallin has a role in controlling FAK function and cardiomyocyte survival through the prevention of calpain-mediated degradation of FAK PMID: 25319025
    9. CryAB attenuated inflammation and oxidative stress in EAM, with the combination of the suppression of excessive CaMKII activity and the subsequent recovery of the Ca2 + homeostasis-related proteins PMID: 25596465
    10. alphaB-crystallin is mobilized early after exposure to a high temperature to interact with damaged proteins but myocardial cells cannot produce sufficient alphaB-crystallin for protection against heat stress. PMID: 24466295
    11. P38 MAPK plays a pro-survival role and confers protection of cardiomyocytes during infarct formation via CRYAB/Nrf2 pathway. PMID: 24464634
    12. Our study revealed the down-regulation of Hsp27 and alphaB-crystallin by trigonelline in adult rats. PMID: 24103517
    13. Alpha B crystallin expression in neurons and astrocytes is upregulated after cerebral hemorrhage. PMID: 23386121
    14. alphaB-crystallin regulates oxidative stress-induced apoptosis in cardiac H9c2 cells via the PI3K/AKT pathway. PMID: 23212619
    15. Alpha crystallin is a moonlighting protein that functions as a heat shock protein as well as a lens crystallin. PMID: 8639509
    16. O-GlcNAcylation of alphaB-crystallin is a dynamic and important regulator of both its localization and function. PMID: 23543138
    17. This comparative analysis with CRYAB and HSP70 demonstrates that differential heat shock response is controlled by cell-type-dependent access of HSF1 and HSF4 to specific promoters, independent of the promoter architecture. PMID: 23264262
    18. Changes were observed in small heat shock proteins HSPB1, HSPB5 and HSPB8 in mitochondria of the failing heart following myocardial infarction in rats PMID: 23546289
    19. CRYAB acts as a regulatory switch in modulating cardiomyocyte apoptosis by mitochondria or endoplasmic reticulum during cardiac hypertrophy or myocardial infarction. PMID: 23559016
    20. The alphaA- and alphaB-crystallin peptides inhibited stress-induced aggregation of four client proteins, and the alphaA-acetyl peptide was more effective than the native peptide against three of the client proteins. PMID: 23508955
    21. CryAB, a small heat shock protein, might prevent the arrhythmogenic effects of particulate matter by attenuating ROS generation and CaMKII activation. PMID: 23153557
    22. alphaA-crystallin and alphaB-crystallin reside in separate subcellular compartments in the developing ocular lens PMID: 23071119
    23. B5 may have various molecular interaction partners at synapses, within dendrites and axons and that this interaction is likely to be regulated by phosphorylation. PMID: 22617993
    24. p38beta confers protection against hydrogen peroxide (HO)-induced astrocyte apoptosis by inducing a small heat shock protein, alphaB-crystallin, which inhibits caspase-3 activation. PMID: 21777656
    25. In soleus muscle, the amount of alphaB-crystallin is ~16 times greater than the amount of heat shock protein (HSP)25 and ~30 times greater than the amount of HSP72. PMID: 21975426
    26. Atg7 induces basal autophagy, rescues the CryAB(R120G) autophagic deficiency, and attenuates the accumulation of misfolded proteins and aggregates in cardiomyocytes PMID: 21617129
    27. Curcumin suppressed the expression of selenite-induced alphaA- and alphaB-crystallin and Hsp 70, and may therefore suppress cataract formation in rat pups PMID: 21311744
    28. The results of this study suggested that expression of alphaB-crystallin and phosphorylated alphaB-crystallin (Ser-59) was significantly increased in the hypothalamic PVN of rats with myocardial infarction compared to sham rats. PMID: 20723582
    29. comparison of tubulin/alphaB-crystalline ratios across the muscles of varied MHC isoforms further suggested that mechanical stress fluctuating at the rhythmic frequency of walking and breathing efficiently activates dynamic function of microtubules. PMID: 19340546
    30. Ischemia-induced association of the stress protein alpha B-crystallin with I-band portion of cardiac titin. PMID: 11945023
    31. These results indicate the presence of differential response mechanisms of alphaB-crystallin gene expression to proteasome inhibition and heat shock. PMID: 14575708
    32. one of the functions of alphaB-crystallin is to bind microtubules via microtubule binding proteins and to give the MTs resistance to disassembly PMID: 15075233
    33. HSF4 binds to alphaB-crystallin, Hsp70, and Hsp82 promoters and has a role in interacting with the canonical heat shock element of the alphaB-crystallin gene PMID: 15308659
    34. alphaB-crystallin effectively inhibits both reactive oxygen species formation and apoptosis in cultured vascular endothelial cells and provide a basis for future therapeutic interventions in diabetic vascular complications. PMID: 15358243
    35. Our results suggest that the p38beta-dependent alpha-BC induction in neurons and astrocytes in the penumbra may play an important role in the postischemic brain. PMID: 15856211
    36. These results indicate that alphaB-crystallin may be regulated by papillary interstitial tonicity in a JNK-dependent process. PMID: 16680485
    37. CryAB-amyloid oligomer formation is interrupted by HSP22 PMID: 17092938
    38. These studies suggest that the association of alphaB-crystallin with actin helps maintenance of pinocytosis, a physiological function essential for survival of cells. PMID: 17196975
    39. Increase in the alphaB-crystallin is associated with sarcopenia PMID: 17761354
    40. alpha A-K11T and alpha B-K90T/K92T mutants showed the largest decrease in glycation and increase in chaperone activity. PMID: 18158587
    41. These findings underline alphaB-crystallin crucial role as a participant of cardiac cells early response to stressful stimuli compromising their survival. PMID: 18420382
    42. Increased expression of alphaB-crystallin early after lesion and statin delivery correlated with increased expression of the heat shock factors 1 and 2. PMID: 18566458
    43. Studies with synchronized human glioblastoma cell line U373MG suggest association of aB-crystallin with cell cycle-dependent Golgi reorganization. PMID: 15339919

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm. Nucleus. Secreted.
  • 蛋白家族：
    Small heat shock protein (HSP20) family
  • 组织特异性：
    Lens as well as other tissues.
  • 数据库链接：

    KEGG: rno:25420

    STRING: 10116.ENSRNOP00000055901

    UniGene: Rn.98208