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  4. Recombinant Rat Apolipoprotein E (Apoe)

Recombinant Rat Apolipoprotein E (Apoe)

  • 货号：
    CSB-YP001936RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP001936RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP001936RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP001936RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    ApoeApolipoprotein E; Apo-E
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    19-312
  • 氨基酸序列
    EG ELEVTDQLPG QSDQPWEQAL NRFWDYLRWV QTLSDQVQEE LQSSQVTQEL TVLMEDTMTE VKAYKKELEE QLGPVAEETR ARLAKEVQAA QARLGADMED LRNRLGQYRN EVNTMLGQST EELRSRLSTH LRKMRKRLMR DADDLQKRLA VYKAGAQEGA ERGVSAIRER LGPLVEQGRQ RTANLGAGAA QPLRDRAQAL SDRIRGRLEE VGNQARDRLE EVREQMEEVR SKMEEQTQQI RLQAEIFQAR IKGWFEPLVE DMQRQWANLM EKIQASVATN SIASTTVPLE NQ
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • 产品描述：
    Recombinant Rat Apolipoprotein E(Apoe) is a partial length protein expressed with an N-terminal His-tagged or Tag-Free. Its expression region corresponds to 19-312aa of rat Apoe protein. Its purity was determined by SDS-PAGE and reached up to 85%. This recombinant Apoe protein may be used to synthesize antibodies against Apoe or on the studies of Apoe-associated signal transduction.
    Apoe mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues. Accumulating evidences have showed that apoE is a key protein linked to the pathogenesis of Alzheimer’s disease (AD) beyond its central roles in lipid metabolism. Additionally, this protein is also implicated in cardiovascular disease.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells.
  • 基因功能参考文献：
    1. Infusion of AngII did not lead to the formation of dissecting abdominal aortic aneurysms or atherosclerosis in ApoE-knockout rats. PMID: 29166645
    2. Results demonstrated that apolipoprotein E4 (ApoE4)exerted direct neurotoxicity and enhanced the neurotoxicity of Abeta1-40 on spatial cognitive function and hippocampal long term potentiation. This may partly elucidate the mechanism by which APOE4 allele exerted negative effects as a major genetic risk factor for developing Alzheimer's disease. PMID: 28356228
    3. renal denervation improves aortic distensibility and attenuated endothelial dysfunction in ApoE(-/-)-rats. PMID: 27277003
    4. The expression of ApoE and S-100 correlated with the progression of focal cerebral contusion. PMID: 26782580
    5. Apolipoprotein E-deficient rats develop atherosclerotic plaques in partially ligated carotid arteries. PMID: 26545012
    6. rats treated with KD (EXP2) showed a significant down-regulated expression of ZnT-3, MT-3, ApoE, clusterin, and ACAT-1 in hippocampus PMID: 25778834
    7. Findings suggest that endogenous apoE generates lower density HDL to produce more lipidated HDL using previously synthesized cholesterol through the interaction with ABCA1 in caveolin-1-rich domain of astrocytes PMID: 24814386
    8. Although rat apolipoprotein E sequence resembles apoE4, an isoform of apoE3, rat apoE displays the biophysical behavior of apoE3. PMID: 23103361
    9. A potential neuroprotective role of apolipoprotein E-containing lipoproteins through low density lipoprotein receptor-related protein 1 in normal tension glaucoma PMID: 22674573
    10. Lower expression of MMP-2 and MMP-9 is associated with the increased expression of apoE in the glomerulus PMID: 22207560
    11. ApoE-containing astrocyte lipoproteins exhibit the most robust interactions with Abeta. PMID: 22138302
    12. Apolipoproteins E3 exerts neurotrophic and synaptogenic effects in dorsal root ganglion cell cultures. PMID: 19414061
    13. LpE and LRP1 promote axonal extension, and suggest that lipids exported to LpE by ABCG1 are important for the enhancement of axonal extension mediated by LpE. PMID: 21040802
    14. ApoE may play a role in lipid rafts or synaptic structural plasticity by the regulation of its levels via dendritic localization and translation. PMID: 20456011
    15. These observations suggest that ApoE, expressed by activated retinal glia, stimulates RGC neurite outgrowth after intravitreal Zymosan injection. PMID: 19382209
    16. Although ApoE increases arterial wall accumulation of triglyceride-rich particles(TGRPs), it also reduces the penetration of TGRPs into the arterial wall--a novel antiatherogenic property. PMID: 12482838
    17. ApoE mRNA was expressed in astrocytes and macrophages, but not in neurons after focal cerebral ischemia PMID: 12870266
    18. Spatiotemporal changes of apolipoprotein E immunoreactivity and apolipoprotein E mRNA expression after transient middle cerebral artery occlusion in rat brain. PMID: 12898539
    19. The Apolipoprotein (apo) E is a protein involved in both lipid metabolism and neuroprotection and its expression system will be highly useful for probing the ability of rat apoE to mediate food intake in rats. PMID: 15866734
    20. Data indicate that the role of apoE4 in cholesterol homeostasis and apolipoprotein cell association is more pronounced in hippocampal neurons. PMID: 16376010
    21. Our studies suggest that AEC I is not just a simple barrier for gas exchange, but a functional cell that protects alveolar epithelium from injury. PMID: 16497717
    22. 24(S)-hydroxycholesterol induces apoE-mediated efflux of cholesterol in astrocytes via an LXR-controlled pathway PMID: 16524875
    23. ApoE expression is not altered during normal brain aging, but there may be a relationship between ApoE and IL-1beta transcription in the cerebral cortex PMID: 16802110
    24. LRP binds and endocytoses Abeta42 both directly and via apoE but endocytosed Abeta42 is not completely degraded and accumulates in intraneuronal lysosomes PMID: 17012232
    25. A naturally processed C-terminal ApoE peptide, Ep1.B, has anti-atherogenic activity indicating a role for this naturally metabolized peptide in vascular wound healing and lipid homeostasis PMID: 17126342
    26. treatment of primary rat mixed glial cell cultures with IL-1beta induced a significant increase in extracellular apoE protein; treatment of primary astrocyte and mixed glial cell cultures with TNF-alpha significantly reduced extracellular apoE PMID: 18288929
    27. mRNA expression of ApoE in arterial walls was not different between the controls and cerebral aneurysms. PMID: 18360691
    28. Data demonstrate that apoE suppresses food intake via a mechanism enhancing melanocortin signaling in the hypothalamus. PMID: 18559657
    29. The finding that hypothalamic apoE and food intake are positively associated during the normal circadian cycle as well as in the period of restricted feeding suggests that hypothalamic apoE is food-entrained and regulates food intake. PMID: 19362542
    30. Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin. PMID: 19717566

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  • 亚细胞定位：
    Secreted. Secreted, extracellular space. Secreted, extracellular space, extracellular matrix.
  • 蛋白家族：
    Apolipoprotein A1/A4/E family
  • 数据库链接：

    KEGG: rno:25728

    STRING: 10116.ENSRNOP00000050968

    UniGene: Rn.32351