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  4. Recombinant Rat Atrial natriuretic peptide receptor 1 (Npr1), partial

Recombinant Rat Atrial natriuretic peptide receptor 1 (Npr1), partial

  • 货号：
    CSB-YP016023RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP016023RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP016023RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP016023RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP016023RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Npr1Atrial natriuretic peptide receptor 1; EC 4.6.1.2; Atrial natriuretic peptide receptor type A; ANP-A; ANPR-A; NPR-A; Guanylate cyclase A; GC-A
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Receptor for the atrial natriuretic peptide NPPA/ANP and the brain natriuretic peptide NPPB/BNP which are potent vasoactive hormones playing a key role in cardiovascular homeostasis. Has guanylate cyclase activity upon binding of the ligand.
  • 基因功能参考文献：
    1. Atenolol treatment normalized the altered expression of Npr1 and Npr2 genes. PMID: 27283501
    2. Suppression of Npr1, not Npr2 gene function induces hypertrophic growth in cardiomyocytes in vitro. PMID: 28743500
    3. The results suggest that an antagonistic cascade exists between the TGF-beta1/Smad/deltaEF1 pathways and Npr1 expression and receptor signaling that is relevant to renal and vascular remodeling, and may be critical in the regulation of blood pressure and cardiovascular homeostasis. PMID: 26934489
    4. BNP and NPR-A are expressed in the dorsal horn ganglion, and expressions are elevated in rats with chronic nonbacterial prostatitis. PMID: 22474982
    5. knockdown of NPR-A up-regulates the expression of NPR-C, Gialpha proteins, and NPR-C-linked adenylyl cyclase signalling and suggests a cross-talk between NPR-A and NPR-C. PMID: 22131352
    6. speculate that, in NPRA, the ANP-induced rotation of the juxtamembrane domains, transmitted across the transmembrane helices, may induce a similar rotation in each of the dimerized GC catalytic domains, leading to the stimulation of GC catalytic activity PMID: 21375693
    7. activation of hypothalamic natriuretic peptide receptor-A in rat has a role in inhibition of dehydration-induced water intake by glucocorticoids PMID: 21187974
    8. Seven sites of phosphorylation were identified in rat GC-A (S497, T500, S502, S506, S510, T513, and S487), and all of these sites except S510 and T513 were observed in human GC-A. PMID: 20977274
    9. The two natriuretic peptide receptors, guanylyl cyclase-A (GC-A) and GC-B are subject to pronounced size alterations in the rat brain between postnatal day 1 and adult. PMID: 20880010
    10. results demonstrate that NPRs are clearly expressed in the cells located in the ganglion cell layer including the three major subtypes of retinal ganglion cells and the expression is mainly on the membrane of somata PMID: 20304036
    11. A new molecular model is proposed for NPRA's active conformation in which the juxtamembrane and transmembrane regions are taken into account before and after receptor activation. PMID: 20214400
    12. These findings support roles for NPR-A, the common receptor of atrial (ANP) and B-type (BNP) natriuretic peptide in mature neurons and provide evidence for pronounced changes in neuronal submembrane cGMP signalling during neuronal differentiation. PMID: 20097258
    13. Study revealed that GC-B predominates in the developing and GC-A in the adult brain, with regional differences each between cerebral cortex, cerebellum, and brain stem. PMID: 19837875
    14. activation of NPRA in relation to eNOS expression in renal medulla cells PMID: 12082097
    15. there are altered NPR-A receptors in rat spontaneously hypertensive kidney, which might act as a negative feedback in response to hypertension PMID: 12217425
    16. A repetitive dinucleotide polymorphism of the Npr1 promotor alters the transcription of the Npr1 gene with consequences on blood pressure in spontaneously hypertensive rats. PMID: 12511524
    17. NPRA-PKG association may have a role in compartmentation of cGMP-mediated signaling and regulation of receptor sensitivity PMID: 12855709
    18. NPRA receptor has a role in attenuating the effects of hypertrophic agents on cardiac wall thickness and in cardiac hypertrophy PMID: 14500707
    19. seminiferous tubules rather than Leydig cells are the predominant GC-A expression sites in the rat testis and functional activity was proved by ANP- induced cGMP accumulation in isolated seminiferous tubules. PMID: 14630722
    20. natriuretic peptide receptor type A has a role in patterning of renal cGMP production PMID: 15093696
    21. The cGMP regulatory region is at position -1372 to -1354 from the transcription start site of Npr1. Cross-competition experiments with mutated oligonucleotides led to the definition of a consensus sequence (-1372 AaAtRKaNTTCaAcAKTY -1354). PMID: 15096467
    22. ANF stimulates renal dopamine uptake through natriuretic peptide-type A receptor PMID: 15544851
    23. The data suggest that pressure overload is accompanied by upregulation of not only ANP and BNP but also their receptors NPR-A and NPR-C in the left ventricle. PMID: 16272201
    24. this is the first study that explores the effect of extracellular Ca+ on ATP-mediated regulation of NPR-A-coupled guanylyl cyclase in renal glomeruli PMID: 17033090
    25. Increase in natriuretic peptide receptor-A expression may account for some of the reported beneficial effect of 1,25(OH)(2)D3 on the cardiovascular system and kidney. PMID: 17440494
    26. our results suggest the existence of a local and complex peptidergic system in the rat testis, involving ANP and its receptors that could importantly modulate the androgen biosynthesis. PMID: 18778744
    27. BNP was capable of inhibiting VSMCs proliferation by NPRA/cGMP pathway PMID: 19104909
    28. protein structure studies showed the fold of the ligand binding domain is that of a bilobal periplasmic binding protein (PBP), which is used in multi-domain receptors/proteins PMID: 11556325

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  • 亚细胞定位：
    Membrane; Single-pass type I membrane protein.
  • 蛋白家族：
    Adenylyl cyclase class-4/guanylyl cyclase family
  • 数据库链接：

    KEGG: rno:24603

    STRING: 10116.ENSRNOP00000020307

    UniGene: Rn.10463