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  4. Recombinant Rat C-C motif chemokine 3 (Ccl3)

Recombinant Rat C-C motif chemokine 3 (Ccl3)

  • 货号：
    CSB-YP004793RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004793RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004793RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004793RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004793RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Ccl3; Mip1a; Scya3; C-C motif chemokine 3; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; Small-inducible cytokine A3
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    24-92
  • 氨基酸序列
    APYGADT PTACCFSYGR QIPRKFIADY FETSSLCSQP GVIFLTKRNR QICADPKETW VQEYITELEL NA
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.
  • 基因功能参考文献：
    1. The important chemokine macrophage inflammatory protein1alpha increases inflammatory responses and the secretion of inflammatory molecules from Kupffer cells, including tumor necrosis factoralpha, interleukin (IL)1beta and IL6. PMID: 28677732
    2. The present report demonstrates an increase in mRNA and protein levels in chemokines CCL3 and its receptor CCR5 in dorsal root ganglion in the rat model of remifentanil-induced hyperalgesia. Furthermore, the results pointed out that CCL3 and its receptor CCR5 might be implicated in remifentanil-induced hyperalgesia. PMID: 26550961
    3. Study shows that erythropoietin reduced chemokine CCL3 mRNA expression in rat cerebellar neuron-enriched preparations during neuroinflammation. PMID: 26178913
    4. The results of this study suggest a contribution of CCL3 in the spinal dorsal horn to paclitaxel-induced allodynia and may provide new therapeutic targets for paclitaxel-induced painful neuropathy. PMID: 25127716
    5. Expression levels of CCL3 in the spinal cord are up-regulated following nerve injury. PMID: 24589480
    6. NMDA-evoked neuronal injury differentially induces MIP-1alpha and MCP-1 production in microglia and astrocytes, respectively, through activation of different intracellular signaling pathways. PMID: 22791363
    7. Our data suggest that free radicals produced in CA1 neurons contribute to the recruitment and activation of microglia and neurodegeneration through MIP-1alpha expression. PMID: 18272692
    8. In this study we present data on long term increase in CCL3 mRNA expression and immunoreactivity following epileptogenic stimulus in a rat model of temporal lobe epilepsy. PMID: 21731074
    9. Data show significant concentration increases for CXCL1 and MIP-1alpha in the brain after soman-induced seizure onset. PMID: 21535896
    10. The active behavior of the MIP-1alpha gene in KCs following liver ischemia/reperfusion injury is assumed to be one of the major causes for the hepatic ischemia/reperfusion injury. PMID: 16804970
    11. P2Y6 activation by uridine 5'-diphosphate induces Ccl2 and Ccl3 expression in primary cultured microglia, astrocytes, and brain slices from neonatal and adult rats. PMID: 21317391
    12. a massive amount of ATP molecules released in the glioma tumor site may act as the regulator with P2XR signaling that increases MIP-1alpha and MCP-1 expression in tumor-infiltrating microglia/macrophages. PMID: 21162127
    13. These results suggest that MIP-1alpha is directly involved in the neurodegeneration induced in rats by PVC-211 murine leukemia virus by recruiting macrophages/microglia from the periphery into regions of the brain that eventually become diseased. PMID: 21051067
    14. expression is regulated by nicotine in alveolar macrophages PMID: 12101081
    15. The constitutive patterns of MIP-1 alpha mRNA and protein expression are demonstrated in rat brain throughout postnatal development within local microenvironments where the postnatal cell migration phenomenon occurs. PMID: 12899200
    16. Oligodendroglia express the CCL3 receptor CCR1, but not CCR5. CCL3 induced an intracellular rise in Ca(2+), a typical signalling event of G protein-coupled receptors. PMID: 14583384

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Intercrine beta (chemokine CC) family
  • 数据库链接：

    KEGG: rno:25542

    STRING: 10116.ENSRNOP00000015139

    UniGene: Rn.10139