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  4. Recombinant Rat Calpastatin (Cast)

Recombinant Rat Calpastatin (Cast)

  • 货号：
    CSB-YP004561RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP004561RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP004561RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP004561RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP004561RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Cast
  • Uniprot No.：
  • 别名：
    Cast; Calpastatin; Calpain inhibitor
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-713
  • 氨基酸序列
    MSRPGPKPAA SSRPRRGAAA SHTQEHVNEK NIGSSSKPAE KKGSDEVTAS SAATGTSPRM STTGAKAVKI ESEKSQSSEP PVIHEKKPKG KPKEGSEPQT LPKHASDTGS KHAHKEKALS RSNEQIVSEK SSESKTKFQD APSADGESVA GGVTVATASD KVVVKKKEKK SLTPTLPMES TLNKLSDKSG VNAALDDLID TLGECEDTNK DDPPYTGPVV LDPMDSTYLE ALGIKEGTIP PEYRKLLEKN EAITGPLPDS PKPMGIDHAI DALSSDFTCS SPTGKQTEKE KSTGESSKAQ SAGVTRSAVP PQEKKRKVEE EVMNDQALQA LSDSLGTRQP DPQSHLRQAK QVKEAKAKEE RQEKCGEDED TVPAEYRLKP AKDKDGKPLL PEPEETSKCL SESELIGELS ADFVQPTYQE KPSMPAAKIK KGVVPDDAVE TLARSLGTRK EDPEDEKSLV DKVKEKAKEE DHEKLGEKEE TIPPDYRLEI VKDKDGKPLL PKEAEEQLPP LSDDFLLDAL SQDFSSPANI LSLGFEDAKL SAAVSETVSQ VPAPSNHTAA PPPGTERRDK ELDDALDELS DSLGQRQPDP DENKPLDDKV KEKIKAEHSE KLGERDDTIP PEYRHLLDND GKDKPEKPLT KNTEKLGQDQ DPIDALSEDL DSCPPTTETS QNTTKEKGKK TSSSKASKNE EKTKDSSKKT EEVPKPKVDE DAT
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Specific inhibition of calpain (calcium-dependent cysteine protease). Plays a key role in postmortem tenderization of meat and have been proposed to be involved in muscle protein degradation in living tissue.
  • 基因功能参考文献：
    1. induction of degradation by caspase-1 in myoblast differentiation and fusion PMID: 12832042
    2. findings support the hypothesis that dysregulation of the calpain-calpastatin system may play a role in the onset of acute pancreatitis PMID: 15132950
    3. Increased calpastatin levels found in juvenile rats muted calpain activity and neuronal apoptosis, following spinal cord injury. PMID: 15453993
    4. there is a discordant expression pattern of cardiac calpastatin mRNA and protein in the hypoxic PMID: 15543935
    5. calpastatin overexpression in L8 myoblasts does not inhibit cell proliferation and alignment, but prevents myoblast fusion and fusion-associated protein degradation PMID: 15904894
    6. CAST overexpression could be used as a therapeutic strategy to prevent progression of liver injury where liver regeneration is severely hampered. PMID: 16871587
    7. Although desmin depletion was not statistical significance, a significant inverse relationship was found between the mu-calpain/calpastatin ratio and the amount of desmin in myofibrils during hindlimb unweighting and reweighting in skeletal muscle. PMID: 17429681
    8. Calpastatin was capable of simultaneously binding four molecules of calpain. PMID: 17543955
    9. It was concluded that the presence of the XL-domain in these isoforms is not relevant for the formation of the calpain/calpastatin complex in the absence of calcium, that is the interaction of calpastatin with inactive calpain. PMID: 17570336
    10. Neuroprotective mechanism of taurine due to up-regulating calpastatin and down-regulating calpain and caspase-3 during focal cerebral ischemia. PMID: 17712625
    11. c-Myc regulates calpain activity through calpastatin; apoptosis induced by calpain inhibition is dependent on c-Myc, and calpastatin knockdown promotes transformation in c-Myc-negative cells PMID: 18544539
    12. In hippocampal slices, both basic excitability and long-term synaptic efficacy are significantly increased upon treatment with a synthetic membrane-permeable activator calpastatin peptide. PMID: 18599308
    13. Role of the calpain-calpastatin system in the density-dependent growth arrest. PMID: 18809371
    14. 3.0 A structure of Ca(2+)-bound m-calpain in complex with the first calpastatin repeat, both from rat, revealing the mechanism of exclusive specificity PMID: 19020622
    15. 2.4-A-resolution crystal structure of the calcium-bound calpain 2 heterodimer bound by one of the four inhibitory domains of calpastatin PMID: 19020623
    16. Over-expression of calpastatin inhibits calpain activation and improves myocardial function in endotoxaemia. PMID: 19318376
    17. calpastatin protein and mRNA levels were low at early developmental time points and increased dramatically by postnatal day 30 PMID: 19751724

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  • 蛋白家族：
    Protease inhibitor I27 (calpastatin) family
  • 数据库链接：

    KEGG: rno:25403

    STRING: 10116.ENSRNOP00000058759

    UniGene: Rn.17481