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  4. Recombinant Rat Cardiac phospholamban (Pln), partial

Recombinant Rat Cardiac phospholamban (Pln), partial

  • 货号：
    CSB-YP018198RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP018198RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP018198RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP018198RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP018198RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Pln; Cardiac phospholamban; PLB
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • 基因功能参考文献：
    1. Long-term in vivo administration of GTE improves cell mechanical properties and intracellular calcium dynamics in normal cardiomyocytes, by increasing energy availability and removing the inhibitory effect of PLB on SERCA2. PMID: 29913456
    2. results confirm that AKAP7gamma/delta binding to PLB is important for phosphorylation of PLB. PMID: 26027516
    3. In reperfused control hearts, inhibition of phospholamban phosphorylation or SERCA (thapsigargin) simulated the cardioprotective effects of ischaemic post-conditioning PMID: 25020913
    4. Diminished phosphorylation of phospholamban by anthrax lethal toxin resulted in acute diastolic heart failure. PMID: 23907041
    5. Time course and mechanisms of phosphorylation of phospholamban residues in ischemia-reperfused rat hearts. Dissociation of phospholamban phosphorylation pathways PMID: 11812163
    6. the interaction between phospholamban phosphorylation and Ca2+ transport across the SR was disrupted during the early phase of sepsis. PMID: 12022759
    7. Maximal inhibition of Ca-ATPase by phospholamban expressed in either slow-twitch skeletal or cardiac muscle is observed at a stoichiometry of 1 mol of Plm per mol of Ca-ATPase. PMID: 12403631
    8. although both phospholamban phosphorylation sites are involved in the mechanical recovery after myocardial ischemia, Thr17 appears to play a major role. PMID: 12763747
    9. Paraplegia significantly increased the relative protein abundances of SERCA (45%) and the Na/Ca exchanger (40%) and decreased phospholamban levels (-28%)in the heart. PMID: 12881214
    10. higher levels of MHC-beta and dephosphorylated PLB may contribute to more contractile dysfunction in male than in female diabetic rat hearts, and that phosphorylation of PLB at threonine 17 is more responsive to insulin in female diabetic rat hearts. PMID: 12933346
    11. phospholamban expression is regulated in cardiac myocytes by adenylyl cyclase VI via activating transcription factor 3 PMID: 15231818
    12. the phosphorylation of Thr17 of phospholamban upon reflow, may favor the full recovery of relaxation after myocardial ischemia-reperfusion injury PMID: 15524173
    13. Beneficial effects of intermittent high altitude hypoxia on threonine phosphorylation and calcium pump. PMID: 15637115
    14. The expression of SLN and PLB mRNA and protein relative to SERCA1 or SERCA2 was assessed in ventricle, atrium, and skeltal msucle of mouse, rat, rabbit and pig. PMID: 15801907
    15. prevent sustained CaMKII autophosphorylation PMID: 17239900
    16. Rescue of Ca2+ overload-induced left ventricular dysfunction by targeted ablation of phospholamban. PMID: 19074672

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Single-pass membrane protein. Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Membrane; Single-pass membrane protein.
  • 蛋白家族：
    Phospholamban family
  • 组织特异性：
    Heart.
  • 数据库链接：

    KEGG: rno:64672

    STRING: 10116.ENSRNOP00000000469

    UniGene: Rn.9740