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  4. Recombinant Rat Cell division control protein 42 homolog (Cdc42)

Recombinant Rat Cell division control protein 42 homolog (Cdc42)

  • 货号：
    CSB-YP806532RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP806532RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP806532RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP806532RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP806532RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cdc42; Cell division control protein 42 homolog; EC 3.6.5.2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-188
  • 氨基酸序列
    MQTIKCVVVG DGAVGKTCLL ISYTTNKFPS EYVPTVFDNY AVTVMIGGEP YTLGLFDTAG QEDYDRLRPL SYPQTDVFLV CFSVVSPSSF ENVKEKWVPE ITHHCPKTPF LLVGTQIDLR DDPSTIEKLA KNKQKPITPE TAEKLARDLK AVKYVECSAL TQKGLKNVFD EAILAALEPP EPKKSRRC
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plasma membrane-associated small GTPase which cycles between an active GTP-bound and an inactive GDP-bound state. In active state binds to a variety of effector proteins to regulate cellular responses. Involved in epithelial cell polarization processes. Regulates the bipolar attachment of spindle microtubules to kinetochores before chromosome congression in metaphase. Regulates cell migration. In neurons, plays a role in the extension and maintenance of the formation of filopodia, thin and actin-rich surface projections. Required for DOCK10-mediated spine formation in Purkinje cells and hippocampal neurons. Facilitates filopodia formation upon DOCK11-activation. Upon activation by CaMKII, modulates dendritic spine structural plasticity by relaying CaMKII transient activation to synapse-specific, long-term signaling. Also plays a role in phagocytosis through organization of the F-actin cytoskeleton associated with forming phagocytic cups.
  • 基因功能参考文献：
    1. Cdc42 may played a role in myocardial I/R via JNK , Bcl-2 and Bax signal pathway. PMID: 28838336
    2. model involving membrane shape changes predicts a localized GTP-Cdc42 pulse results in nucleation of a cortical patch PMID: 29321558
    3. role of Cdc42 and Rac1 activities in pheochromocytoma, the adrenal medulla tumor PMID: 27355516
    4. Polarity signaling via CDC42/atypical protein kinase C can affect the dynamic turnover of the intermediate filament network to promote the polarization of the network itself. PMID: 28432079
    5. studies demonstrate that TGase-catalyzed transamidation and activation of rac1 and cdc42 results from stimulation of multiple types of receptors and this novel signaling pathway can regulate dendritic spine morphology and plasticity. PMID: 28161375
    6. These findings reveal the activation of Cdc42 in embryonic spinal commissural axon growth cones and support the conclusion that src family kinase activation downstream of deleted in colorectal cancer is required for Cdc42 activation by netrin-1. PMID: 27482713
    7. These results indicate that HuR promotes early intestinal mucosal repair after injury by increasing Cdc42 translation. PMID: 28031329
    8. Axin promotes dendritic spine stabilization through Cdc42-dependent cytoskeletal reorganization. PMID: 26204446
    9. Results indicate that Cdc42 plays a key role in the epileptic seizure of rats induced with pilocarpine; Cdc42 inhibitor, ML141, reduces seizure severity, delays latency of first seizure and improves both the mIPSCs and eIPSCs in hippocampal slices PMID: 25595978
    10. Interaction of Cdc42-TGFbeta3 regulates filopodial structures and and epithelial cell adhesion. PMID: 23750457
    11. These results establish cell-autonomous and stage-specific functions for the small Rho GTPases Cdc42 and Rac1 in the course of adult hippocampal neurogenesis. PMID: 23325254
    12. The altered melusin pathways and CDC42 parallel the cardiac function progression during cardiac remodeling post-MI. PMID: 21546274
    13. Culturing NSCs on increasingly stiff ECMs enhances RhoA and Cdc42 activation, increases NSC stiffness, and suppresses neurogenesis. PMID: 21956892
    14. These findings suggest that Cdc42 facilitates exocytosis by modulating both the number of exocytosis-competent dense-core vesicles and the production of dense-core vesicles in PC12 cells. PMID: 22426478
    15. Acurate regulation of Cdc42 activity is crucial for proper equatorial actin assembly and RhoA localization during cytokinesis in epithelial cells. PMID: 21763307
    16. Cdc42 controls the cholesterol-regulated transport and localization of NPC1L1, and plays a role in cholesterol absorption. PMID: 21844200
    17. Manipulation of the individual Rho GTPases showed differential effects on survival, with little variation in their migratory route and predominant differentiation into the oligodendroglial lineage. PMID: 19943951
    18. Results demonstrate influence of membrane dynamics on the localization and activation of Cdc42 and consequently on directed cell migration. PMID: 21173111
    19. TSC2 has a role in controlling cell polarity and migration by regulating CDC42 and RAC1 activation PMID: 20530489
    20. Both cdc42-dependent and independent pathways modulate neuritic cofilin activity downstream from amyloid beta-induced rods in brain slices. PMID: 19542631
    21. role in cyclooxygenase-2 gene expression PMID: 11829737
    22. Cdc42 regulates GSK-3beta and adenomatous polyposis coli to control cell polarity PMID: 12610628
    23. Cdc42-induced targeting at cell-cell contacts is dependent on CEACAM1 PMID: 14517298
    24. Activation of Rac1 and Cdc42 was caused by both guanine nucleotide exchange factors and GAPs, and was important for neurite extension. PMID: 14570905
    25. Findings suggest that Rin-mediated neurite outgrowth requires not only Rac/Cdc42 activation but also Rin-calmodulin association, and that Rin is involved in calcium/CaM-mediated neuronal signaling. PMID: 14662747
    26. role of the Rac/Cdc42-IQGAP1 system in the dynamic organization and maintenance of the E-cadherin-based adherens junctions PMID: 15263019
    27. Interactions among IQGAP1, Cdc42, and beta-catenin are crucial to the regulation of Sertoli-germ cell, but not Sertoli-Sertoli cell, adherens junction dynamics in the seminiferous epithelium. PMID: 15389538
    28. PAR-6-PAR-3 complex mediates Cdc42-induced Rac activation by means of STEF/Tiam1 PMID: 15723051
    29. Cdc42 has profound effects on the recruitment of dynein to COPI vesicles. Cdc42, when bound to coatomer, inhibits dynein binding to COPI vesicles whereas preventing the coatomer-Cdc42 interaction stimulates dynein binding. PMID: 15866890
    30. Activation of Rac-1 and Cdc42 stabilizes microvascular endothelial barrier functions in vitro and in vivo, likely by increasing the junction-associated actin cytoskeleton. PMID: 16195887
    31. Produces signals that are essential for the neurite outgrowth of PC12 cells and cerebellar granule neurons. PMID: 16328953
    32. Inhibition of cdc42 in nigral neurons blocked medial forebrain bundle axotomy-induced activation (phosphorylation) of MAP kinase kinase 4 and c-Jun, resulting in attenuation of substantia nigra pars compacta neuronal death. PMID: 16336220
    33. active state of Cdc42 is important in upregulation of phospholipase D1 activity which is responsible for the increase of neurite outgrowth PMID: 16842757
    34. Study concludes that Scrib plays a key role in the establishment of cell polarity during migration; by interacting with betaPIX, Scrib controls localization and activation of the small GTPase Cdc42 and regulates Cdc42-dependent polarization pathways. PMID: 17081755
    35. Results show that phenylalanine reduced the number of dendrites and dendritic spines in cultured neurons, and that phenylalanine down-regulated Rac1, Cdc42, and RhoA mRNA and protein expression. PMID: 17515837
    36. Data suggest that Cdc42 activation is crucial for the regulation of actin polymerization in carcinoma cells, and required for both EGF-stimulated protrusion and cell motility independently of effects on Rac. PMID: 17855387
    37. These data describe a novel role for Rho in coordinating signaling by Rac and Cdc42. PMID: 18316075
    38. Cdc42 and RhoA have opposing roles in regulating membrane type 1-matrix metalloproteinase localization and matrix metalloproteinase-2 activation. PMID: 18562481
    39. neuronal guanine nucleotide exchange factor, intersectin-1L, activates the GTPase Cdc42, which in turn provides de novo actin filaments that are important for calcium-regulated exocytosis in PC12 cells PMID: 19161392
    40. Rab35 colocalizes with actin filaments and with Cdc42, Rac1 and RhoA, and that Rab35 can activate Cdc42 both in vivo and in vitro PMID: 19289122

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  • 亚细胞定位：
    Cell membrane; Lipid-anchor; Cytoplasmic side. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle. Cytoplasm. Cell projection, lamellipodium membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, dendrite.
  • 蛋白家族：
    Small GTPase superfamily, Rho family, CDC42 subfamily
  • 数据库链接：

    KEGG: rno:64465

    STRING: 10116.ENSRNOP00000030928

    UniGene: Rn.228625