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  4. Recombinant Rat Chymase (Cma1)

Recombinant Rat Chymase (Cma1)

  • 货号：
    CSB-YP005599RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005599RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005599RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005599RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005599RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cma1; Mcpt3Chymase; EC 3.4.21.39; Alpha-chymase; Mast cell protease 3; rMCP-3; Mast cell protease 5; rMCP-5; Mast cell protease III; rMCP-III
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-247
  • 氨基酸序列
    IIGGTECIP HSRPYMAYLE IVTSDNYLSA CSGFLIRRNF VLTAAHCAGR SITVLLGAHN KTYKEDTWQK LEVEKQFIHP NYDKRLVLHD IMLLKLKEKA KLTLGVGTLP LSANFNFIPP GRMCRAVGWG RTNVNEPASD TLQEVKMRLQ EPQSCKHFTS FQHKSQLCVG NPKKMQNVYK GDSGGPLLCA GIAQGIASYV HPNAKPPAVF TRISHYRPWI NKILREN
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.
  • 基因功能参考文献：
    1. The pathobiologic response in WKY rats was directly linked to increases in cardiac gene expression and enzymatic activity of chymase and modest reductions in ACE2 activity. PMID: 28748720
    2. Compared to chymase, ACE-mediated angiotensin-(1-12) metabolism in the heart was several fold lower. Overall our findings suggest that angiotensin-(1-12), not Ang I, is the better substrate for Ang II formation by chymase in adult rats. PMID: 27465904
    3. In a pure VO model, chymase produced in adult cardiac fibroblasts leads to autophagic degradation of newly synthesized intracellular procollagen I, suggesting a new role of chymase in extracellular matrix degradation. PMID: 26807691
    4. The results indicate an important role for chymase in inducing fibrosis through TGF-beta1 activation, parallel with Ang II effects. PMID: 22875344
    5. Chymase-secreting mast cells mediate apoptosis of neighboring smooth muscle cells through a mechanism involving degradation of pericellular fibronectin and disruption of the p-focal adhesion kinase-dependent cell-survival signaling cascade. PMID: 12588765
    6. rat alpha-chymase has converted to elastase-like substrate specificity, perhaps associated with an adoption of new biological targets PMID: 12900423
    7. Based on the results, this study suggested rMCP-5 is a key promoter of angiogenesis in the rat. PMID: 15723097
    8. Plasma ANG II does not appear to regulate intrarenal chymase activity. ACE-independent ANG II production by chymase may provide mechanism for augmenting intrarenal ANG II in ischemic kidney in renovascular hypertension. PMID: 15869764
    9. By disrupting the NF-kappaB-mediated survival-signaling pathway, activated chymase-secreting mast cells can mediate apoptosis of cultured arterial SMCs. PMID: 16460729
    10. Angiotensin II-forming chymase may play a role in the proliferation of the medial layer in the lung arterioles of monocrotaline-induced pulmonary hypertensive rats. PMID: 16690960
    11. These results suggest that chymase can promote cardiac fibroblast proliferation and collagen synthesis via TGF-beta 1/Smad pathway rather than angiotensin II, which is implicated in the process of myocardial fibrosis. PMID: 18057996
    12. chymase inactivates the FAK-mediated cell survival signaling PMID: 18079408

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  • 亚细胞定位：
    Secreted. Cytoplasmic granule. Note=Secretory granules.
  • 蛋白家族：
    Peptidase S1 family, Granzyme subfamily
  • 组织特异性：
    Mast cells.
  • 数据库链接：

    KEGG: rno:25627

    STRING: 10116.ENSRNOP00000061330

    UniGene: Rn.10182