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  4. Recombinant Rat Class E basic helix-loop-helix protein 40 (Bhlhe40)

Recombinant Rat Class E basic helix-loop-helix protein 40 (Bhlhe40)

  • 中文名称：
    Recombinant Rat Class E basic helix-loop-helix protein 40(Bhlhe40),Yeast
  • 货号：
    CSB-YP002691RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Rat Class E basic helix-loop-helix protein 40(Bhlhe40),Yeast
  • 货号：
    CSB-EP002691RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Rat Class E basic helix-loop-helix protein 40(Bhlhe40),Yeast
  • 货号：
    CSB-EP002691RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Rat Class E basic helix-loop-helix protein 40(Bhlhe40),Yeast
  • 货号：
    CSB-BP002691RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Rat Class E basic helix-loop-helix protein 40(Bhlhe40),Yeast
  • 货号：
    CSB-MP002691RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Bhlhe40
  • Uniprot No.：
  • 别名：
    Bhlhe40; Bhlhb2; Sharp2Class E basic helix-loop-helix protein 40; bHLHe40; Class B basic helix-loop-helix protein 2; bHLHb2; Enhancer-of-split and hairy-related protein 2; SHARP-2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-411
  • 氨基酸序列
    MERIPSAQPP PTCLPKTPGL EHGDLSGMDF AHMYQVYKSR RGIKRSEDSK ETYKLPHRLI EKKRRDRINE CIAQLKDLLP EHLKLTTLGH LEKAVVLELT LKHVKALTNL IDQQQQKIMA LQSGLQAGDL SGKNIEAGQE MFCSGFQTCA REVLQYLAKH ENTRDLKSSQ LVTHLHRVVS ELLQGSASRK PLDSAPKPVD FKEKPSFLAK GSEGPGKNCV PVIQRTFAPS GGEQSGSDTD TDSGYGGELE KGDLRSEQPY FKSDHGRRFT VGERVSTIKQ ESEEPPTKKS RMQLSDEEGH FVGSDLMGSP FLGPHPHQPP FCLPFYLIPP SATAYLPMLE KCWYPTSVPL LYPGLNTSAA ALSSFMNPDK IPTPLLLPQR LPSPLAHSSL DSSALLQALK QIPPLNLETK D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes. Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1/2 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes. Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA and NR1H3/LXRA transactivation activity. May be involved in the regulation of chondrocyte differentiation via the cAMP pathway. Represses the transcription of NR0B2 and attentuates the transactivation of NR0B2 by the CLOCK-ARNTL/BMAL1 complex. Drives the circadian rhythm of blood pressure through transcriptional repression of ATP1B1 in the cardiovascular system.
  • 基因功能参考文献：
    1. Bhlhb2 expression was significantly higher in prefrontal cortex of the Attention Deficit Hyperactivity animal model compared to controls. PMID: 26820676
    2. insulin induces the expression of the rat SHARP-2 gene at the transcription level via both a PI 3-K/aPKClambda- and a PI 3-K/mTOR- pathways PMID: 27792527
    3. 5-Aminoimidazole-4-carboxyamide-1-beta-D-ribofuranoside-induction of the SHARP-2 gene is mediated at transcription level by a phosphoinositide 3-kinase/atypical protein kinase C lambda pathway. PMID: 26590300
    4. Results describe significant dissociation of expression patterns with arrhythmic expression of Dec1 and rhythmic expression of Dec2 in rat kidney under a normal light-dark (LD) cycle. PMID: 21430201
    5. Dec1 and Dec2 are regulators of the mammalian molecular clock, and form a fifth clock-gene family [dec1 & dec2] PMID: 12397359
    6. insulin induces the transcription of the rat SHARP-2 gene via a phosphoinositide 3-kinase pathway. PMID: 12796501
    7. the level of SHARP-2 mRNA is regulated by gonadotropins and SHARP-2 functions as a transcriptional repressor localized in the nucleus. PMID: 12954728
    8. The hepatic expression of SHARP-2 mRNA was investigated under various conditions. PMID: 14725860
    9. SHARP-2 is a potential repressor of phosphoenolpyruvate carboxykinase gene expression PMID: 15733865
    10. BHLHB2 and ChREBP constitute a novel feedback loop involved in the regulation of lipogenesis. PMID: 18602890

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 组织特异性：
    Expressed in heart, spleen, lung, liver, muscle, kidney, uterus and gut. Highly expressed in the cerebral cortex, especially in the fifth layer, thalamus, superior colliculus, olfactory bulb, piriform cortex, hippocampus and hypothalamic nuclei.
  • 数据库链接：

    KEGG: rno:79431

    STRING: 10116.ENSRNOP00000009564

    UniGene: Rn.81055