Your Good Partner in Biology Research

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Rat Clusterin (Clu)

Recombinant Rat Clusterin (Clu)

  • 货号：
    CSB-YP005595RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005595RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005595RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005595RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005595RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    CluClusterin; Dimeric acid glycoprotein; DAG; Sulfated glycoprotein 2; SGP-2; Testosterone repressed prostate message 2; TRPM-2) [Cleaved into: Clusterin beta chain; Clusterin alpha chain]
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    22-447
  • 氨基酸序列
    EQEFSDNEL QELSTQGSRY VNKEIQNAVQ GVKHIKTLIE KTNAERKSLL NSLEEAKKKK EGALDDTRDS EMKLKAFPEV CNETMMALWE ECKPCLKHTC MKFYARVCRS GSGLVGRQLE EFLNQSSPFY FWMNGDRIDS LLESDRQQSQ VLDAMQDSFT RASGIIDTLF QDRFFTHEPQ DIHHFSPMGF PHKRPHFLYP KSRLVRSLMP LSHYGPLSFH NMFQPFFDMI HQAQQAMDVQ LHSPALQFPD VDFLKEGEDD PTVCKEIRHN STGCLKMKGQ CEKCQEILSV DCSTNNPAQA NLRQELNDSL QVAERLTQQY NELLHSLQSK MLNTSSLLEQ LNDQFTWVSQ LANLTQGDDQ YLRVSTVTTH SSDSEVPSRV TEVVVKLFDS DPITVVLPEE VSKDNPKFMD TVAEKALQEY RRKSRME
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Functions as extracellular chaperone that prevents aggregation of non native proteins. Prevents stress-induced aggregation of blood plasma proteins. Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro). Does not require ATP. Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70. Does not refold proteins by itself. Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation. When secreted, protects cells against apoptosis and against cytolysis by complement. Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins. Promotes proteasomal degradation of COMMD1 and IKBKB. Modulates NF-kappa-B transcriptional activity. Following stress, promotes apoptosis. Inhibits apoptosis when associated with the mitochondrial membrane by interference with BAX-dependent release of cytochrome c into the cytoplasm. Plays a role in the regulation of cell proliferation. An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5. Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3. Plays a role in the clearance of immune complexes that arise during cell injury.
  • 基因功能参考文献：
    1. clusterin might be involved in the formation of tubular complexes and parenchymal regeneration during rat pancreatitis PMID: 30111670
    2. Results suggest that an increase in the secretion of clusterin by Sertoli cells protects the testes from heat stress-induced injury. PMID: 25661013
    3. ApoJ serves as a cytoprotective protein in neonatal rat ventricular cells against cytotoxicity of AngII through the PI3K-Akt-ROS and MAPK/ NF-kappaB pathways. PMID: 26391229
    4. rats treated with KD (EXP2) showed a significant down-regulated expression of ZnT-3, MT-3, ApoE, clusterin, and ACAT-1 in hippocampus PMID: 25778834
    5. Following restraint stress, hypothalamic and pituitary clusterin expression levels are augmented. PMID: 24080898
    6. Exendin-4 treatment induces new beta cell clusters via up-regulation of clusterin, which might be effective on beta-cell proliferation and neogenesis. PMID: 23351716
    7. Nulear clusterin plays a pro-apoptotic role in ethanol-induced cell death in the developing brain. PMID: 21762182
    8. Cox-2 induces clusterin in a 15d-PGJ(2)-dependent manner. PMID: 22138303
    9. attenuates renal fibrosis in obstructive nephropathy PMID: 22052058
    10. The results suggested that clusterin may contribute to gastrin's growth-promoting effect on the oxyntic mucosa. PMID: 21995960
    11. bisphenol A upregulated clusterin expression in atrophic prostate epithelial cells and induced lipid peroxidation and DNA fragmentation in spermatozoa. PMID: 21536718
    12. clusterin is an important mediator of neural precursor cell-derived neuronal survival and differentiation PMID: 21212797
    13. The chaperone clusterin mediates systemic clearance of misfolded extracellular proteins PMID: 21505792
    14. The chaperone action of clusterin is independent of ATP PMID: 11123922
    15. The chaperone action of clusterin targets prefibrillar species to inhibit amyloid formation PMID: 17412999
    16. The extracellular chaperone clusterin appears in the cytosol during endoplasmic reticulum stress PMID: 17451556
    17. The chaperone action of clusterin is important in quality control of extracellular protein folding PMID: 19878774
    18. The chaperone clusterin inhibits aggregation and precipitation of major human plasma proteins PMID: 19996109
    19. Clusterin is an extracellular holdase-type chaperone PMID: 10066740
    20. The chaperone action of clusterin is like that of the small heat shock proteins PMID: 10694874
    21. Urinary levels serve as a marker for the severity of tubular damage and may help to differentiate between glomerular and tubular injuries. PMID: 12457227
    22. clusterin production and secretion, particularly by astrocytes, could be neuroprotective-perhaps via its putative actions such as inhibition of complement activation and cytolysis, effects on chemotaxis and apoptosis, and actions as an anti-stress protein PMID: 12782389
    23. clusterin can be involved in neurodegeneration and cell survival PMID: 12799419
    24. Role for TRPM2 in the calcium signaling of activated microglia. PMID: 14512294
    25. After retinal ischemia, some Muller cells die by apoptosis; clusterin produced and released by Muller cells may play an important role in the pathogenesis of ischemic injury in the rat retina. PMID: 15139011
    26. The role of clusterin and its isoforms in SMC behavior is complex and chronologically regulated in response to microenvironmental changes. Proliferative arrest reduces s-CLU; apoptosis increases n-CLU. PMID: 15591223
    27. clusterin protects cardiomyocytes against ischemic cell death via a complement-independent pathway PMID: 15994859
    28. Sclip interacts with clusterin in PC12 cells and the interaction may act as an important modulator during neuronal differentiation. PMID: 16038898
    29. These observations suggest that clusterin induction is NF-kappaB-independent and unrelated to the inflammatory response in N9 microglia. PMID: 16157419
    30. Accumulation of aberrant proteins, caused by the proteasome inhibitor MG132 or the incorporation of the amino acid analogue AZC (L-azetidine-2-carboxylic acid), increased both clusterin protein and mRNA levels in the human glial cell line U-251 MG. PMID: 16336210
    31. results suggest that clusterin can be considered as a potential morphogenic factor that promotes differentiation of pancreatic beta cells PMID: 16411126
    32. Molecular pathological evaluation of clu in a rat model of unilateral ureteral obstruction was made as a possible biomarker of nephrotoxicity. PMID: 17455085
    33. clusterin overexpression is associated with the up-regulation of the phosphorylation of Akt PMID: 18321852
    34. Regulation of clusterin expression in the prostate of aging rats is complex and is neither directly repressed by androgen nor dependent on apoptotic-induced stress PMID: 18942093
    35. Results indicate that secreted clusterin expression in diabetic nephropathy was induced due to renal tissue damage, and the nuclear clusterin expression increase in renal tubuli was related to apoptosis. PMID: 18949565
    36. upregulation of clusterin during vascular injury may be a protective response against, rather than a causative response to, the development of neointimal hyperplasia. PMID: 19696405

    显示更多

    收起更多

  • 亚细胞定位：
    Secreted. Nucleus. Cytoplasm. Mitochondrion membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytosol. Microsome. Endoplasmic reticulum. Mitochondrion. Mitochondrion membrane. Cytoplasm, perinuclear region. Cytoplasmic vesicle, secretory vesicle, chromaffin granule.
  • 蛋白家族：
    Clusterin family
  • 组织特异性：
    Detected in Sertoli cells (at protein level). Detected in cultured Sertoli cells, testis, epididymis, liver and brain.
  • 数据库链接：

    KEGG: rno:24854

    STRING: 10116.ENSRNOP00000022095

    UniGene: Rn.1780