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  4. Recombinant Rat Complexin-1 (Cplx1)

Recombinant Rat Complexin-1 (Cplx1)

  • 货号：
    CSB-YP005893RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP005893RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP005893RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP005893RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP005893RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cplx1Complexin-1; Complexin I; CPX I; Synaphin-2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-134
  • 氨基酸序列
    MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEPE EEDESILDTV IKYLPGPLQD MFKK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
  • 基因功能参考文献：
    1. actively drives SNARE complexes into linker-open conformation PMID: 30194295
    2. Results suggest that the C-terminal domain of Cplx1 plays a role in preferential membrane localization of Cpx to synaptic vesicles and find it as an important regulator for synaptic vesicle fusion through promoting Ca2+ in conjunction with SNAREs and synaptotagmins. PMID: 27821736
    3. Complexin-1 inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms. PMID: 25122624
    4. complexins modulate neuroexocytosis after assembly of membrane-bridging SNARE complexes PMID: 11751907
    5. CPX I plays a critical role in beta-cells in the control of the stimulated-exocytosis of insulin. PMID: 15126625
    6. Transient increases in the levels of complexin I and complexin II proteins were detected in the injured cerebral cortex 6 h following TBI. This increase was followed by a decrease of complexin I in the injured cortex and hippocampus. PMID: 16430375
    7. Results indicate that distinct functional domains of copmplexin I differentially regulate synaptic exocytosis, and that CplxI carries out a crucial role in fine-tuning Ca2+-triggered fast neurotransmitter release. PMID: 17828276
    8. Data show direct interaction of SNARE complex binding protein synaphin/complexin with calcium sensor synaptotagmin 1. PMID: 19132534

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  • 亚细胞定位：
    Cytoplasm, cytosol. Cell junction, synapse, presynapse. Perikaryon.
  • 蛋白家族：
    Complexin/synaphin family
  • 组织特异性：
    Nervous system. Strongly expressed in brain, where it is predominant in neurons from cerebral cortex and thalamus (at protein level).
  • 数据库链接：

    KEGG: rno:64832

    STRING: 10116.ENSRNOP00000058432

    UniGene: Rn.10133