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  4. Recombinant Rat Cytochrome P450 2E1 (Cyp2e1)

Recombinant Rat Cytochrome P450 2E1 (Cyp2e1)

  • 货号：
    CSB-YP006425RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP006425RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP006425RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP006425RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP006425RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Cyp2e1; Cyp2e; Cyp2e-1Cytochrome P450 2E1; EC 1.14.14.1; 4-nitrophenol 2-hydroxylase; EC 1.14.13.n7; CYPIIE1; Cytochrome P450-J; Cytochrome P450RLM6
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    2-493
  • 氨基酸序列
    AVLGITIAL LVWVATLLVI SIWKQIYNSW NLPPGPFPLP ILGNIFQLDL KDIPKSFTKL AKRFGPVFTL HLGSRRIVVL HGYKAVKEVL LNHKNEFSGR GDIPVFQEYK NKGIIFNNGP TWKDVRRFSL SILRDWGMGK QGNEARIQRE AQFLVEELKK TKGQPFDPTF LIGCAPCNVI ADILFNKRFD YNDKKCLRLM SLFNENFYLL STPWIQLYNN FADYLRYLPG SHRKIMKNVS EIKQYTLEKA KEHLQSLDIN CARDVTDCLL IEMEKEKHSQ EPMYTMENVS VTLADLFFAG TETTSTTLRY GLLILMKYPE IEEKLHEEID RVIGPSRVPA VRDRLDMPYM DAVVHEIQRF INLVPSNLPH EATRDTVFQG YVIPKGTVVI PTLDSLLYDS HEFPDPEKFK PEHFLNENGK FKYSDYFKAF SAGKRVCVGE GLARMELFLL LSAILQHFNL KSLVDPKDID LSPVTVGFGS IPPQFKLCVI PRS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.
  • 基因功能参考文献：
    1. acute Formaldehyde (FA) poisoning suppressed the activities of CYP2C11, CYP2E1, and CYP3A2 and induced the activity of CYP1A2 in rats. And the change of CYP450 activity caused by acute FA poisoning may be associated with FA potential carcinogenic effects on the airways. PMID: 28555194
    2. Data suggest that combined treatment with non-nutritive sweetener aspartame and insulin in a rat model of juvenile diabetes induces CYP2E1 in neurons of cerebrum and cerebellum without modifying CYP2E1 in liver, while CYP3A2 is induced in cerebrum/cerebellum and in liver. PMID: 25038063
    3. Impaired growth hormone-mediated signaling is observed in ethanol-exposed hepatocytes and is explained by differential effects of alcohol dehydrogenase (ADH)- and cytochrome P450 2E1 (CYP2E1)-mediated ethanol metabolism on the Jak2/STAT5B pathway. PMID: 28864499
    4. The results suggest that changes in coumarin pharmacokinetics and CYP2E1 subcellular distribution contribute to resistance to coumarin-induced hepatic necrosis, while cytotoxicity of metabolic conjugates shown in vitro may contribute to bile duct damage upon repeated coumarin administration. PMID: 28803882
    5. The isoniazid-LPS-induced inflammation model might prove valuable in revealing the hepatotoxic mechanisms of isoniazid and the crucial role played by CYP2E1 in the initiation and propagation of isoniazid-induced liver damage. PMID: 27324775
    6. The CYP2E1-PPARalpha axis may play a role in ethanol-induced neurotoxicity via the alteration of the genes related with synaptic function PMID: 28385499
    7. The results suggested that kaempferol exhibited a weak inhibition of CYP2E1 activity with an IC50 of 60.26 +/- 2.54 muM, while quercetin and kaempferol caused a moderate inhibition of CYP3A4 activity with IC50 values of 18.77 +/- 1.69 muM and 32.65 +/- 1.32 muM, respectively. Isoquercitrin and astragalin had no effects on the activities of either CYP2E1 or CYP3A4. PMID: 27314315
    8. Subcellular localization of CYP2E1 results in distinctly different enzyme activities that could impact overall metabolic clearance and/or activation of substrates and thus impact the interpretation and prediction of toxicological outcomes. PMID: 26463279
    9. Modulation of testicular cytochrome P450 2E1 mRNA and protein expression could change the functioning of this isozyme in target organs and take part in the mechanism of ethanol gonadotoxicity. PMID: 23819932
    10. This study showed significant changes in the activity and protein and mRNA expression of CYP2E1 or CYP3A1 in rats after exposure to chronic high altitude hypoxia. PMID: 25330250
    11. Data suggest that Cyp2e1 is induced in testes during infertility as the result of either experimental type I diabetes or experimental alcoholism; level of Cyp2e1 mRNA in testes correlates with defect in spermatogenesis. PMID: 25323755
    12. Schizonepetin induced the expression of CYP3A1, CYP1A2 and CYP2E1 at dosages of 24 and 48 mg/kg. PMID: 23235327
    13. In testes of isoniazid-treated rats CYP2E1 may act as a trigger in generating of reactive oxygen species and other toxic metabolites which subsequently mediates DNA damage, spermatogenesis disturbances, and altered male fertilizing capacity. PMID: 25683034
    14. During CYP2E1-induced oxidative stress, transcription factor Nrf2 has a role in the early adaptive response. Inhibition of CYP2E1 coupled with the prior induction of Nrf2 may be a tool to reduce CYP2E1-mediated rhabdomyolysis-induced acute kidney injury. PMID: 24717297
    15. The results demonstrate that CYP2E1 induces iNOS-independent free radical generation and subsequently modulates the Nrf2-dependent HO-1 and 4-HNE-mediated GST expression in maneb- and paraquat-treated PMNs. PMID: 24771067
    16. CYP2E1 and NRF2 are introduced as negative regulators of GLUT4 expression and function in insulin-sensitive cells. PMID: 24500986
    17. Overexpression of CYP2E1 enhances tetrandrine-induced cytotoxicity in hepatocytes. PMID: 23892269
    18. Insulin regulates CYP2E1 through the PI3-K, Akt, mTOR signaling pathway. PMID: 23920219
    19. Butadiene metabolites reversibly, but not irreversibly, inhibit CYP2E1 metabolic activity; no difference in butadiene metabolite inhibition of rat and mouse CYP2E1 activity PMID: 24021170
    20. The expression of hepatic cytochrome P450 2E1 in rats with immune hepatic fibrosis is not significantly different from the normal rats. Blueberry has no effect on the expression of hepatic cytochrome P4502E1. PMID: 23044233
    21. Garlic oil can effectively reduce the peripheral nerve injury induced by n-hexane due to the decreased content and activity of hepatic CYP2E1. PMID: 22357518
    22. The protein levels of CYP 2c6, 2e1 and 2j3 increased in the ischemic region of the rat hearts, while the mRNA levels of CYPs were unchanged. PMID: 22885143
    23. Soy protein may improve alcohol-induced lipid accumulation, oxidative stress and inflammation by decreasing proinflammatory cytokines and CYP2E1 protein expression and by increasing PPARalpha and CYP4A protein expressions and fecal lipid excretion. PMID: 21813270
    24. Results suggest that damage to cytochrome c oxidase (CcO) and dissociation of respirosome complexes are critical factors in alcohol-induced toxicity, which is augmented by mitochondrion-targeted cytochrome CYP2E1. PMID: 22396533
    25. Rubus alceaefolius poiron could protect liver cells from injury, and inhibit the mRNA expressions of CYP2E1 and CYP3A1 in liver tissue. PMID: 19848203
    26. Alkaloids from R alceifolius Poir may act to protect the liver through decreasing CYP2E1 enzymatic activity. PMID: 21224420
    27. CYP2E1 induces oxidative stress and augments alcohol-mediated cell/tissue injury PMID: 20529841
    28. Data demonstrated a significantly decreased Cyp2E1 mRNA expression in patients with fibrotic and inflammatory lung diseases compared to healthy controls. PMID: 20514434
    29. HMR1766 reduces oxidative stress, decreased CYP2E1 levels, and normalized vasomotor function in diabetic rats. PMID: 20522591
    30. dilinoleoylphosphatidylcholine decreases ethanol-induced cytochrome P4502E1 PMID: 11689017
    31. increased translation of collagen mRNA by CYP2E1-derived reactive oxygen species is responsible for the increase in collagen protein PMID: 11782477
    32. GH-deficient rats show an altered inducibility PMID: 11884239
    33. Influence of acetaminophen and trichloroethylene on liver cytochrome P450-dependent monooxygenase system PMID: 11996102
    34. CYP2E1-dependent production of reactive oxygen species enhances lipid peroxidation when AA is added to hepatocytes. PMID: 12029627
    35. N-terminal 30 amino acids of CYP2E1 contain a chimeric signal for bimodal targeting of the apoprotein to endoplasmic reticulum and mitochondria PMID: 12191992
    36. determination of 16-nucleotide sequence is implicated in the CYP2E1 post-transcriptional regulation by insulin PMID: 12270935
    37. Expression induces hepatocyte resistance to cell death from oxidative stress in hepatocytes. PMID: 12470497
    38. Diffusable CYP2E1-derived oxidative-stress mediators induce synthesis of laminin gamma 1 by increased Sp1-dependent transactivation in hepatic stellate cells. PMID: 12529372
    39. inflammatory cytokines inhibit CYP2E1 expression by control of HNF-1alpha function and regulation of other transcriptional factors acting on the CYP2E1 5'-upstream regulatory region. PMID: 12604681
    40. P450 2E1 has a role in inducing heme oxygenase-1 through ERK MAPK pathway PMID: 12777398
    41. An amino acid stretch in rat CYP2E1 including the B-helix from glycine 82 to asparagine 95 is responsible for mitochondrial association of rat CYP2E1 expressed in yeast. PMID: 14661969
    42. 5-8 fold increase of cytochrome P450 2E1 (CYP2E1) and glutathione S-transferase (GST) A4-4 levels in mitochondria from streptozotocin diabetic rat tissues compared with normal values (glutathione transferase A4-4) PMID: 14693714
    43. chronic CYP2E1 overexpression in hepatocytes leads to sustained ERK1/2 activation mediated by EGFR/c-Raf signaling. PMID: 14767997
    44. CYP2E1 is a unique gene expressing in liver but did not express in hepatocellular carcinoma PMID: 15162526
    45. We studied CYP2E1 transcriptional regulation in glial cells and identified a repressor element at +1452/+1460 in intron 2 of the rat CYP2E1 gene which helps control basal expression levels of CYP2E1, and may modulate the response to inflammatory factors PMID: 15189336
    46. increased hepatocyte CYP2E1 expression and the presence of steatohepatitis result in the down-regulation of insulin signaling PMID: 15632182
    47. Inhibited by s-adenosylmethionine(SAM) but not likely to play a major role in ability of SAM to protect against hepatotoxicity. PMID: 15763544
    48. CYP2E1 is capable of inducing significant endoplasmic reticulum protein damage and stress via its catalytic activation of pro-oxidants PMID: 15797236
    49. In conclusion, contrasting effects of SAMe on CYP2E1 toxicity were observed in pyrazole hepatocytes and E47 cells. PMID: 16306132
    50. Data show that treatment of fibroblasts expressing CYP2E1 with the hsp90 inhibitor radicicol results in CYP2E1 degradation that is inhibited by the proteasome inhibitor lactacystin. PMID: 16331994

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  • 亚细胞定位：
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. Mitochondrion inner membrane; Peripheral membrane protein.
  • 蛋白家族：
    Cytochrome P450 family
  • 数据库链接：

    KEGG: rno:25086

    STRING: 10116.ENSRNOP00000016883

    UniGene: Rn.1372