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  4. Recombinant Rat DNA (cytosine-5)-methyltransferase 3-like (Dnmt3l)

Recombinant Rat DNA (cytosine-5)-methyltransferase 3-like (Dnmt3l)

  • 中文名称：
    大鼠Dnmt3l重组蛋白
  • 货号：
    CSB-YP631881RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Dnmt3l重组蛋白
  • 货号：
    CSB-EP631881RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Dnmt3l重组蛋白
  • 货号：
    CSB-EP631881RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Dnmt3l重组蛋白
  • 货号：
    CSB-BP631881RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Dnmt3l重组蛋白
  • 货号：
    CSB-MP631881RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Dnmt3l
  • Uniprot No.：
  • 别名：
    Dnmt3lDNA; cytosine-5)-methyltransferase 3-like
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-422
  • 氨基酸序列
    MGSRETPSSC SKTHETLNLE TPESSSTDPD SPLEEQWPKS APDLKEEDSM DMVLEDSKEP LTPSSPPTGR EVIRYEVNVN QRNIEDICLC CGSLQVYAQH PLFEGGICAP CKDKFLETLF LYDEDGHQSY CTICCSGHTL FICESPDCTR CYCFECVDIL VGPGTSERIN AMACWVCFLC LPFSRSGLLQ RRKKWRHQLK AFHDREGASP VEIYKTVSAW KRQPVRVLSL FGNIDKELKS LGFLESSSGS EGGTLKYVED VTNVVRREVE KWGPFDLVYG STQPLGYSCD RCPGWYMFQF HRILQYARPR QDSQQPFFWI FVDNLLLTED DQETTVRFLQ TEAVTLQDVR GRVLQNAMRV WSNIPGLKSK HADLTPKEEQ SLQTQVRTRS KLAAQKVDSL VKYCLLPLRE YFKYFSQNSL PL
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context. Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain. Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3. Plays a key role in embryonic stem cells and germ cells. In germ cells, required for the methylation of imprinted loci together with DNMT3A. In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization. Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation. While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes. Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes. Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression.
  • 基因功能参考文献：
    1. in the F1 and F2 vinclozolin generation (E16) embryonic testis, but the majority returned to control-generation levels by the F3 generation. The most dramatic effects were on the germ-line-associated Dnmt3A and Dnmt3L PMID: 18042343
  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: rno:309680

    STRING: 10116.ENSRNOP00000001610

    UniGene: Rn.129905