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  4. Recombinant Rat DNA-binding protein inhibitor ID-2 (Id2)

Recombinant Rat DNA-binding protein inhibitor ID-2 (Id2)

  • 货号：
    CSB-YP010967RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP010967RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP010967RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP010967RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP010967RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    Id2; Id-2; DNA-binding protein inhibitor ID-2; Inhibitor of DNA binding 2; Inhibitor of differentiation 2
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-134
  • 氨基酸序列
    MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVT KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQTSR TPLTTLNTDI SILSLQASEF PSELMSNDSK VLCG
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
  • 基因功能参考文献：
    1. Here, the s discovered that, in the developing neuron from rat, Akt signaling regulates axon growth and growth cone formation through phosphorylation of serine 14 (S14) on Inhibitor of DNA binding 2 (Id2). PMID: 27938661
    2. Knockdown of Id2 alleviates neuropathic pain by inhibiting the NF-kappaB activation. PMID: 26768262
    3. The data support the implication of inhibitor of DNA binding/differentiation 2 in the modulation of hypoxia/ischemia-induced neuronal apoptosis. PMID: 26187693
    4. Hypoxia/ischemia (H/I) up-regulates Id2 expression in neuronal cells, and Id2 might play an important role in H/I-induced neuronal apoptosis. PMID: 23994058
    5. NF-kappaB p65 can facilitate early fracture healing of rat radius by elevating PGE2 production and regulating BMP-7 and Id2 expression. PMID: 21675116
    6. CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell PMID: 22041901
    7. A novel transcriptional-dependent mechanism of Id2 regulation by GSH and oxidative stress induced by acetaminophen-overdose through the indirect modulation of the proteasome pathway. PMID: 20815767
    8. Id2 protein is integrated into the cell cycle regulatory cascade that results in SMC proliferation following vascular injury and suggest that this effect is at least in part via a cdk2-dependent inhibition of p21 gene expression. PMID: 11706002
    9. induction of Id2 and resultant inhibition of basic helix-loop-helix transcription factor-regulated genes in Sertoli cells may contribute to the regulation of spermatogenesis PMID: 14761970
    10. Id2 & p53 were elevated in cytosolic fraction of suspended muscle in young & aged animals but not in the nuclear protein fraction; findings are consistent with the hypothesis that Id2 & p53 are responsive to unloading-induced muscle atrophy PMID: 16282427
    11. The results indicated that Id2 mRNA is expression in these regions include the amygdaloid complex, caudate putamen, globus pallidus, substantia nigra pars reticulata, suprachiasmatic nucleus, and the anterior part of the subventricular zone. PMID: 16443197
    12. Hypoxia did inhibit the downregulation of the trophoblast stem cell marker Id2. PMID: 16481593
    13. A control role for Id2 in hepatocyte priming, through a p130 dissociation-independent regulation of c-myc, in regenerating rat liver is reported. PMID: 16776654
    14. These observations suggest that Id2 may be mainly involved in terminal maturation of oligodendrocytes and myelination. PMID: 18029094
    15. TSH regulates Id2 expression, but that Id2 does not alter the expression of thyroid-specific genes, such as Tg, in FRTL-5 thyroid cells PMID: 18437010

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  • 亚细胞定位：
    Cytoplasm. Nucleus.
  • 数据库链接：

    KEGG: rno:25587

    STRING: 10116.ENSRNOP00000009491

    UniGene: Rn.3272