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  4. Recombinant Rat Death domain-associated protein 6 (Daxx)

Recombinant Rat Death domain-associated protein 6 (Daxx)

  • 货号：
    CSB-YP845039RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP845039RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP845039RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP845039RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP845039RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
  • Uniprot No.：
  • 别名：
    DaxxDeath domain-associated protein 6; Daxx
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-731
  • 氨基酸序列
    MATDDSIIVL DDDDEDEAAA QPGPSNPASN PVSPGPEASG PSESHGDGGS SNSGSRKCYK LENEKLFEEF LELCKMQTSD HPEVVPFLHK LQQRAQSAFL ASAEFRNILS RVLSRSRNRP AKLYVYINEL CTVLKAHSIK KKLNLAPAAS AESSGDNPPT DPPSDLTNTE TTASEASRTR GSRRQIQRLE QLLALYVAEI QRLQEKELDL SELDDPDSSY LQEARLKRKL IRLFGRLCDL KDCSSLTGKV IEQRIPYRGT RYPEVNRRIE RLINKPGPDT FPDYGDVLRA VEKAATRHSL GLPRQQLQLL AQDAFRDVGV RLQERRHLDL IYNFGCHLTD DYRPGVDPAL TDPTLARRLR ENRTLAMSRL DEVISKYAMM QDKSEEGERQ KRRARLLATS QSSDLPKASS DSGEGPSGVA SQEDPTTPKA ETEDEEDDEE SDDEEEEEEE EEEEATEDED EDLEQLQEDQ DDEEEEEGDN EDDKSPASPS PIFRRKEFSN PQKGSGPQEE QQERGLTGTP ASPLEASPGL PSTDAESSGE QLQERLLAGE SPVSQLSELD MEALPEETIP SPEERGISSS RRKSDSSLPT ILENGAAMVT STSFNGRVSS HPCRDASPPS KRFRKEKKQL GPGPLGNSYV KKQTMAQQDS GWKISVLSTP SSPLASVGPV ADSSTRVDSP SHELVTSSLC NPSPSLILQT PQSQSPRPCI YKTSVATQCD PEEIIVLSDS D
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription corepressor known to repress transcriptional potential of several sumoylated transcription factors. Down-regulates basal and activated transcription. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Inhibits transcriptional activation of PAX3 and ETS1 through direct protein-protein interactions. Modulates PAX5 activity; the function seems to involve CREBBP. Acts as an adapter protein in a MDM2-DAXX-USP7 complex by regulating the RING-finger E3 ligase MDM2 ubiquitination activity. Under non-stress condition, in association with the deubiquitinating USP7, prevents MDM2 self-ubiquitination and enhances the intrinsic E3 ligase activity of MDM2 towards TP53, thereby promoting TP53 ubiquitination and subsequent proteasomal degradation. Upon DNA damage, its association with MDM2 and USP7 is disrupted, resulting in increased MDM2 autoubiquitination and consequently, MDM2 degradation, which leads to TP53 stabilization. Acts as histone chaperone that facilitates deposition of histone H3.3. Acts as targeting component of the chromatin remodeling complex ATRX:DAXX which has ATP-dependent DNA translocase activity and catalyzes the replication-independent deposition of histone H3.3 in pericentric DNA repeats outside S-phase and telomeres, and the in vitro remodeling of H3.3-containing nucleosomes. Does not affect the ATPase activity of ATRX but alleviates its transcription repression activity. Upon neuronal activation associates with regulatory elements of selected immediate early genes where it promotes deposition of histone H3.3 which may be linked to transcriptional induction of these genes. Required for the recruitment of histone H3.3:H4 dimers to PML-nuclear bodies (PML-NBs); the process is independent of ATRX and facilitated by ASF1A; PML-NBs are suggested to function as regulatory sites for the incorporation of newly synthesized histone H3.3 into chromatin. Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Plays a role as a positive regulator of the heat shock transcription factor HSF1 activity during the stress protein response.
  • 亚细胞定位：
    Cytoplasm. Nucleus, nucleoplasm. Nucleus, PML body. Nucleus, nucleolus. Chromosome, centromere.
  • 蛋白家族：
    DAXX family
  • 数据库链接：

    STRING: 10116.ENSRNOP00000000559

    UniGene: Rn.870