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  4. Recombinant Rat E3 ubiquitin-protein ligase rififylin (Rffl)

Recombinant Rat E3 ubiquitin-protein ligase rififylin (Rffl)

  • 中文名称：
    大鼠Rffl重组蛋白
  • 货号：
    CSB-YP814556RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大鼠Rffl重组蛋白
  • 货号：
    CSB-EP814556RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大鼠Rffl重组蛋白
  • 货号：
    CSB-EP814556RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大鼠Rffl重组蛋白
  • 货号：
    CSB-BP814556RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大鼠Rffl重组蛋白
  • 货号：
    CSB-MP814556RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Rffl
  • Uniprot No.：
  • 别名：
    Rffl; E3 ubiquitin-protein ligase rififylin; EC 2.3.2.27; FYVE-RING finger protein Sakura; RING finger and FYVE-like domain-containing protein 1; RING-type E3 ubiquitin transferase rififylin
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-362
  • 氨基酸序列
    MWASCCNWFC LDGQPEETPP PQGARTQAYS NPGYSSFPSP TGSEPSCKAC GVHFASTTRK QTCLDCKKNF CMTCSSQEGN GPRLCLLCLR FRATAFQREE LMKMKVKDLR DYLSLHDIST EMCREKEELV FLVLGQQPVI SEADRTRAPT LPQAFPEQQA FLTQPQSSTV PPTSPGLPSS PAQVTSVLAQ DQETQQAIGH VSQDHEEPIF LESTARVPPE DETQSVDSED SFVPGRRASL SDLTHLEDIE GLTVRQLKEI LARNFVNYKG CCEKWELMER VTRLYKDQKG LQHLVSGNED QNGGAVPSGL EENLCKICMD SPIDCVLLEC GHMVTCTKCG KRMNECPICR QYVIRAVHVF RS
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    E3 ubiquitin-protein ligase that regulates several biological processes through the ubiquitin-mediated proteasomal degradation of various target proteins. Mediates 'Lys-48'-linked polyubiquitination of PRR5L and its subsequent proteasomal degradation thereby indirectly regulating cell migration through the mTORC2 complex. Also ubiquitinates the caspases CASP8 and CASP10, promoting their proteasomal degradation, to negatively regulate apoptosis downstream of death domain receptors. Also negatively regulates the tumor necrosis factor-mediated signaling pathway through targeting of RIPK1 to ubiquitin-mediated proteasomal degradation. Negatively regulates p53/TP53 through its direct ubiquitination and targeting to proteasomal degradation. Indirectly, may also negatively regulate p53/TP53 through ubiquitination and degradation of SFN. May also play a role in endocytic recycling.
  • 基因功能参考文献：
    1. Targeted disruption of the rat Rffl-lnc1 locus caused aberrant, short QT-intervals and elevated blood pressure. Further, to specifically examine the significance of the 19bp polymorphism within the Rffl-lnc1 locus, a CRISPR/Cas9 based targeted knock-in rescue model was constructed by inserting the 19bp into the strain which contained the deletion polymorphism. PMID: 28827789
    2. our study provides evidence to suggest that early perturbations in endocytic recycling caused by the overexpression of Rffl is a novel physiological mechanism potentially underlying the development of hypertension. PMID: 21357277
    3. identified two novel Ub ligases, Momo and Sakura, carrying double zinc finger motif and RING finger domain in rat brain; Sakura expression is more widely detected in the brain white matter as well as in many peripheral organs PMID: 12859687
  • 亚细胞定位：
    Cytoplasm, cytosol. Cell membrane; Peripheral membrane protein. Recycling endosome membrane; Peripheral membrane protein. Note=The FYVE-type zinc finger may mediate phosphatidylinositol phosphate-binding and control subcellular localization.
  • 组织特异性：
    Ubiquitous. Detected in cerebrum, cerebellum, midbrain, brain stem, hippocampus, striatum, liver, heart, lung, kidney, muscle, spleen and testis.
  • 数据库链接：

    KEGG: rno:282844

    STRING: 10116.ENSRNOP00000045245

    UniGene: Rn.159718