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  4. Recombinant Rat Eukaryotic translation initiation factor 3 subunit J (Eif3j)

Recombinant Rat Eukaryotic translation initiation factor 3 subunit J (Eif3j)

  • 货号：
    CSB-YP007539RA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 货号：
    CSB-EP007539RA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 货号：
    CSB-EP007539RA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 货号：
    CSB-BP007539RA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 货号：
    CSB-MP007539RA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Eif3j
  • Uniprot No.：
  • 别名：
    Eif3j; Eif3s1Eukaryotic translation initiation factor 3 subunit J; eIF3j; Eukaryotic translation initiation factor 3 subunit 1; eIF-3-alpha; eIF3 p35
  • 种属：
    Rattus norvegicus (Rat)
  • 蛋白长度：
    Full length protein
  • 表达区域：
    1-259
  • 氨基酸序列
    MAAAAAAAAG DSDSWDADTF SMEDPVRKVA GGGTAGGDRW EGEDEDEDVK DNWDDDDDEN KEEAEVKPEV KISEKKKIAE KIKEKERQQK KRQEEIKKRL EEPEESKVLT PEEQLADKLR LKKLQEESDL ELAKETFGVN NTVYGIDAMN PSSRDDFTEF GKLLKDKITQ YEKSLYYASF LEALVRDVCI SLEIDDLKKI TNSLTVLCSE KQKQEKQSKA KKKKKGVVPG GGLKATMKDD LADYGGYDGG YVQDYEDFM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA.
  • 基因功能参考文献：
    1. Knockdown of eIF-3-alpha enhances the expression of differentiation markers in PC12 cell. PMID: 22025682
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    EIF-3 subunit J family
  • 数据库链接：

    KEGG: rno:691947

    STRING: 10116.ENSRNOP00000022437

    UniGene: Rn.162681